Bordetella (para)pertussis

Molecular detection of Bordetella (para)pertusis DNA
Lab Test name Contact Comments
UZ Brussel - Microbiology Bordetella (para)pertussis Dr. Denis Piérard, Oriane Soetens
SAMPLE TYPE:
-preferential: nasopharyngeal aspirate (min. 0.2 ml)
-alternative: nasopharyngeal swab
FREQUENCY: every weekday
TEST TYPE: In-house real-time PCR
TARGETS: IS481, IS1001, RECA en IS1002
Version date: 26/09/2017
Accredited:
UZA Mol. Diagn. Bordetella (para)pertussis D. Ursi, K. Bergs, M. Michiels, V. Matheeussen
Sample type:
-respiratory specimens (swab, nasopharyngeal aspirate, sputum,...)
(at least 250 µl).

Frequency: 3 times a week

Test type: in house real-time PCR. Only B. pertussis.
CHU UCL NAMUR Bordetella (para)pertussis pierre.bogaerts@uclouvain.be
Bordetella pertussis only.
Sample type
-preferential: nasopharyngeal aspirate (min. 200 µL)
-alternative: nasopharyngeal swab
Tests performed on Tuesday and Friday.
In house real-time PCR.
ZNA Middelheim Bordetella (para)pertussis Walter Verstrepen
Sample type:
-respiratory specimens (nasal swab, nasopharyngeal aspirate, sputum,...)
(at least 250 µl). Throat swabs are not suitable.

Frequency: every working day
Test type: in house real-time PCR
Detection of B. parapertussis included
GZA St-Augustinus Bordetella (para)pertussis Philippe Van Lint
This PCR test is performed on respiratory samples (500µL).
Frequency: daily (Monday-Friday)

This respiratory panel screens for the following targets:
Viruses: Adenovirus, Bocavirus, Coronavirus, Enterovirus, Human metapneumovirus, (Para-)Influenza virus, Rhinovirus, RSV.
Bacteria: Bordetella (para-)pertussis, Bordetella holmesii, Chlamydophila pneumoniae, Legionella pneumophila, Mycoplasma pneumoniae.
Imelda-Bonheiden Bordetella (para)pertussis Dr. Johan Frans, Isabel Micalessi
Sample Type:
Nasopharyngeal swab (eSwab) (minimum 250µl)
Frequency: 2x/week
Test type: Real-time PCR (Diagenode Bordetella Real-time PCR)
AZ St-Lucas Gent Bordetella (para)pertussis Dr. A.M van den Abeele/Elke Vanlaere
Only Bordetella pertussis

SAMPLE TYPE:
-preferential: nasopharyngeal aspirate (min. 0.2 ml)
-alternative: BAL
FREQUENCY: 2x/week
TEST TYPE: Smartfinder (Pathofinder)
MDU Maasstad (NL) Bordetella (para)pertussis Anneke van der Zee
IS481, IS1001, and IS1002 PCR for Bordetella pertussis, B.parapertussis, B.holmesii. In-house PCR on respiratory samples. Roorda et al. BMC Research Notes 2011, 4:11.
voor meer informatie: klik op het icoontje van de link.
OLVZ Aalst Bordetella (para)pertussis Anne Vankeerberghen
Sample type:
Pharyngal swab
Respiratory sample (500 µl)

Frequency: Daily (except weekend)
Test type: In house real time PCR
Version date: 29/09/2017
AZ St-Jan Brugge - Laboratoriumgeneeskunde Bordetella (para)pertussis Dr. M. Reynders, P. Descheemaeker
SAMPLE TYPE:
respiratoiry samples (min 550 µl)
FREQUENCY:
daily
TEST TYPE: TaqMan Array Card, in house real time PCR(PtxS1, IS481, IS1001)
Version date: 17/11/2017
Accredited:
Jessa Hospital Bordetella (para)pertussis dr. Cartuyvels, Raymaekers Marijke
SAMPLE TYPE:
-nasopharyngeal aspirate or secretion
-nasopharyngeal e-swab (UTM or liquid amies)
-BA
FREQUENCY: Monday till Friday
TEST TYPE: in-house real time PCR
Version date: 06/11/2017
Accredited: