Mycobacterium tuberculosis

Molecular detection of Mycobacterium tuberculosis
Lab Test name Contact Comments
AZ St-Jan Brugge - Laboratoriumgeneeskunde Mycobacterium tuberculosis Dr. E. Nulens / Patrick Descheemaeker
SAMPLE TYPE:
- Respiratory sample
- extra-respiratory sample
- urine
- paraffine coupes (microtoom)
FREQUENCY: 2x/week (Monday/Thursday)
TEST TYPE: BDProbeTec™ ET Mycobacterium tuberculosis complex (DTB)
Version date: 17/11/2017
Accredited:
UZA Mol. Diagn. Mycobacterium tuberculosis D. Ursi, K. Bergs, M. Michiels, V. Matheeussen
Sample type:
- respiratory specimens (swab, nasopharyngeal aspirate, sputum,...)
- other fluids (gastric,..)
(at least 250 µl).
- biopsies (not fixed!)

Frequency: 2 times a week

Test type: in house real-time PCR
Jessa Hospital Mycobacterium tuberculosis dr. Cartuyvels, Raymaekers Marijke
SAMPLE TYPE:
-sputum
-bronchoalveolar lavage
-CSF
-fluids (gastric fluid,...)
-biopsy
FREQUENCY: from Monday till Friday
TEST TYPE: GeneXpert (Cepheid)
Accredited:
OLVZ Aalst Mycobacterium tuberculosis Anne Vankeerberghen
Sample type:
CSF (min 5 ml)
Urine(min 40 ml)
Pleura fluid (10 ml)
Respiratory sample(500 µl)
Tissue/biopsy

Frequency: 1-2/week
Test type: In house real time PCR
Version date: 29/09/2017
Accredited:
Ziekenhuis Oost-Limburg Mycobacterium tuberculosis Dr. Els Oris, Katrien Vandebroek
Sample type:
Respiratory specimens

Frequency:
Daily

Test type:
Xpert MTB/RIF (cepheid)
CHU UCL NAMUR Mycobacterium tuberculosis pierre.bogaerts@uclouvain.be
Sample type:
- respiratory specimens (BAL, nasopharyngeal aspirate, sputum,...)
- other fluids (gastric,...)
(at least 250 µl).
- not fixed biopsies
1x/week; possibility of urgent processing after biologist agreement
BD ProbeTec
CHU UCL NAMUR Mycobacterium tuberculosis pierre.bogaerts@uclouvain.be
Sample type: culture
On demand
AccuProbe
UZ Leuven MolPLUZ Mycobacterium tuberculosis Katrien Lagrou
Sample type:
- respiratory samples
- liquid cultures

Minimal sample volume:2 ml
Frequency: 2 times a week (Tuesday-Friday)
Test type: In-house realtime PCR
Remark: Result for Mycobacterium tuberculosis complex and atypical Mycobacteria
CHU Saint-Pierre Mycobacterium tuberculosis Reynders Marijke, Scheen Robert, Maaroufi Younes
Staaltypes: Respiratoire monsters (BAL, tracheobroncheolaire aspiraten, sputum, nasopharynxaspiraten), maagtubages, pleuraal vocht, ascitesvocht, CSV, biopten.

Minimum staalvolume: 0,5 mL (uitzonderlijk: voor CSV minimum 250 microliter)

Techniek: Cobas Taqman MTB (Roche)
ZNA Middelheim Mycobacterium tuberculosis Kim Camps
Sample type: respiratory samples
Frequency:twice a week
Test type: commercial kit (COBAS AmpliPrep - Taqman 48)
GZA St-Augustinus Mycobacterium tuberculosis Philippe Van Lint
performed on respiratory samples

in-house, qualitative real-time PCR

requency: daily (Monday-Friday)
Accredited:
UZ Brussel - Microbiology Mycobacterium tuberculosis Denis Pierard, Oriane Soetens
Sample type:
-Sputum, BAL (min 1 ml)
-CSV (min 1 ml)
Frequency: every weekday
Test type: Commercial real-time PCR (Xpert MTB/RIF, Cepheid)
Version date: 26/09/2017
Imelda-Bonheiden Mycobacterium tuberculosis Dr. Frans, Isabel Micalessi
Sample Type:
- BAL
- Sputum
Frequency: Daily
Test Type: Xpert MTB/RIF (GeneXpert - Cepheid)