Mycoplasma pneumoniae

Molecular detection of Mycoplasma pneumoniae
Lab Test name Contact Comments
AZ St-Jan Brugge - Laboratoriumgeneeskunde Mycoplasma pneumoniae Dr. M. Reynders / Patrick Descheemaeker
SAMPLE TYPE:
respiratoiry samples (min 550 µl)
FREQUENCY:
daily
TEST TYPE:
respiratoiry samples: TaqMan Array Card, in house real time PCR
Version date: 17/11/2017
UZA Mol. Diagn. Mycoplasma pneumoniae D. Ursi, K. Bergs, M. Michiels, V. Matheeussen
Sample type:
- respiratory specimens (swab, nasopharyngeal aspirate, sputum,...)
- cerebrospinal fluid
(at least 250 µl).

Frequency: 2 times a week

Test type: in house real-time PCR
OLVZ Aalst Mycoplasma pneumoniae Anne Vankeerberghen
Sample type:
Pharyngal swab
Respiratory sample (500 µl)

Frequency: Daily (except weekend)

Test type: In house real time PCR
Version date: 06/05/2019
Accredited:
Ziekenhuis Oost-Limburg Mycoplasma pneumoniae Dr. Els Oris, Katrien Vandebroek
Sample type:
nasopharyngeal aspirate

Frequency:
Daily (except weekend)

Test type:
In house real time PCR
CHU UCL NAMUR Mycoplasma pneumoniae pierre.bogaerts@uclouvain.be
Sample type:
- respiratory specimens (swab, nasopharyngeal aspirate, sputum, BAL,...)
- cerebrospinal fluid
(at least 250 µl).
Tests performed on Tuesday and Friday.
In house real-time PCR.
UZ Leuven MolPLUZ Mycoplasma pneumoniae Katrien Lagrou
Emmanuel Andre
Sample type:
- respiratory swab
- respiratory samples
Minimal sample volume: 1ml
Frequency: every weekday
Test type: In-house realtime PCR
Remark: also included in respiratory-panel-assay on swab, aspirate and BAL
Accreditation BELAC: 215-MED
Accredited:
CHU Saint-Pierre Mycoplasma pneumoniae Reynders Marijke, Maaroufi Younes, Scheen Robert
Staaltypes: respiratoire stalen (BAL, sputum, tracheobroncheolaire aspiraten, nasopharynxaspiraten)

Minimum staalvolume: 250 microliter

Kwalitatieve in house real-time PCR
ZNA Middelheim Mycoplasma pneumoniae Walter Verstrepen
Sample type:
- respiratory specimens (swab, nasopharyngeal aspirate, sputum,...)
- cerebrospinal fluid
(at least 250 µl).

Frequency: every working day
Test type: in house real-time PCR
GZA St-Augustinus Mycoplasma pneumoniae Philippe Van Lint
This PCR test is performed on respiratory samples (500µL).
Frequency: daily (Monday-Friday)

The respiratory PCR is part of the following syndromic panel:
Seegene RV Master and Seegene Pneumobacter assay

Viruses
Influenza A virus
Influenza B virus
Human respiratory syncytial virus
Human metapneumovirus
Human adenovirus
Human rhinovirus
Human parainfluenza virus
SARS-CoV-2

Bacteria
Streptococcus pneumoniae
Legionella pneumophila
Haemophilus influenzae
Bordetella parapertussis
Mycoplasma pneumoniae
Bordetella pertussis
Chlamydophila pneumonia
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This PCR test is performed on CSV.
Frequency: daily (Monday-Saturday)

The CSV PCR is part of the following syndromic panel: QIAstat-Dx Meningitis/Encephalitis (ME) Panel

Bacteria
Streptococcus pneumoniae
Neisseria meningitidis
Streptococcus agalactiae
Listeria monocytogenes
Haemophilus influenzae
Escherichia coli K1
Streptococcus pyogenes
Mycoplasma pneumoniae

Viruses
Enterovirus
Herpes simplex virus 1
Herpes simplex virus 2
Humaan parechovirus
Humaan herpes virus 6
Varicella zoster virus

Fungi
Cryptococcus gattii/neoformans

BELAC accreditation number 404-MED
Accredited:
Labo Cliniques Sud Luxembourg Mycoplasma pneumoniae Nicolas Hougardy
SAMPLE TYPE:
- BAL (min 1ml)
- Swabs
FREQUENCY: 1x/day (except weekend)
TEST TYPE: "Mycoplasma pneumoniae Chlamydophila pneumoniae Real-Time PCR" (Diagenode), LightCycler (Roche)
Imelda-Bonheiden Mycoplasma pneumoniae Dr. Johan Frans, Isabel Micalessi
Sample Type:
- NAP in UTM
- BAL
Frequency: Daily
Test Type: NxTAG Respiratoir Pathogeen Panel (MAGPIX, Luminex)
AZ St-Lucas Gent Mycoplasma pneumoniae Dr. A.M van den Abeele/Elke Vanlaere
SAMPLE TYPE:
-preferential: nasopharyngeal aspirate (min. 0.2 ml)
-alternative: BAL
FREQUENCY: 2x/week
TEST TYPE: Smartfinder (Pathofinder)
Jessa Hospital Mycoplasma pneumoniae dr Cartuyvels, Raymaekers Marijke
SAMPLE TYPE:
-nasopharyngeal aspirate or secretion
-nasopharyngeal e-swab (UTM or liquid amies)
-BA
FREQUENCY: Monday till Friday
TEST TYPE: in-house real time PCR
Accredited:
AZ Damiaan Oostende Mycoplasma pneumoniae Ben Vanmassenhove/ Anne-Sophie Hervent
SAMPLE TYPE: flocked swabs
FREQUENCY: 1-3/week
TEST TYPE: Allplex RP4 (Seegene)
BELAC accreditation number 433-MED
Version date: 17/05/2021
Accredited:
Jan Yperman Ziekenhuis Mycoplasma pneumoniae Bieke Decaesteker,
Stijn Jonckheere
Sample Type: Cerebrospinal fluid
Frequency: Daily
Test Type: RT-PCR; QIAStat-Dx Meningitis/Encephalitis panel (QIAStat, Qiagen)

The ME test also screens for the following targets:
Viruses: Enterovirus, Herpes Simplex virus 1, Herpes Simplex virus 2, Human parechovirus, Human herpes virus 6, Varicella zoster virus
Bacteria: Streptococcus pneumoniae, Neisseria meningitidis, Streptococcus agalactiae, Listeria monocytogenes, Haemophilus influenza, Escherichia coli K1, Streptococcus pyogenes
Fungi: Cryptococcus neoformans/gattii

Sample type: respiratory samples
Frequency: daily
Test Type: RT-PCR; QIAStat-Dx Respiratory SARS-CoV-2 panel (QIAStat, Qiagen)

The respiratory test also screens for the following targets:
Viruses: Adenovirus, Bocavirus, Coronavirus, Enterovirus, Human metapneumovirus, (Para-)Influenza virus, Rhinovirus, RSV.
Bacteria: Bordetella (para-)pertussis, Legionella pneumophila

Belac accreditation number 523-MED
Version date: 07/07/2022
Accredited:
AZ St-Lucas Brugge Mycoplasma pneumoniae Dr Karel Maelegheer
Sample type: respiratory sample (300 µl)
Frequency: 1-3/week
Allplex PneumoBacter Assay (Seegene)
Version date: 25/10/2022