BRAF V600E mutation analysis (hematology)

Lab Test name Contact Comments
UZ Leuven MolPLUZ BRAF V600E mutation analysis (hematology) Lucienne Michaux / Els Lierman
Sample type: blood / bone marrow (EDTA, 2-5 ml)
Frequency: 2x/week
Test type: digital droplet PCR (ddPCR) on DNA with PrimePCR ddPCR Mutation Assay (Bio-Rad) for the p.V600E mutation in BRAF.
Accredited:
AZ St-Jan Brugge - Laboratoriumgeneeskunde BRAF V600E mutation analysis (hematology) dr. Helena Devos/Friedel Nollet, Ph.D.
method : real time PCR with blocking LNA primer. Reproducible sensitivity 1%
Version date: 04/11/2017
Accredited:
AZ Delta BRAF V600E mutation analysis (hematology) Geert Martens/Franceska Dedeurwaerdere/Joke Breyne
SAMPLE TYPE: blood or bone marrow (1ml, EDTA); FFPE tissue
FREQUENCY: 1x/week
TEST TYPE: Next Generation Sequencing
Version date: 05/12/2017
Accredited:
CUB Erasme - Medical Genetics Dpt BRAF V600E mutation analysis (hematology) Pierre Heimann/Barbara Dessars/Hakim El Housni
Real-Time fluorescent ARMS-PCR

Analytical sensitivity : 0.5%
UZ Gent BRAF V600E mutation analysis (hematology) Barbara Denys,
Karl Vandepoele
real-time in house assay on DNA
CHU Liège - Hemato BRAF V600E mutation analysis (hematology) Dr .Sc. Sabine FRANKE
5ml EDTA bone marrow or blood.
Hairy Cell Leukemia differential.

Pyrosequencing.