CEBPalpha mutation analysis

Lab Test name Contact Comments
CHU Liège - Hemato CEBPalpha mutation analysis Dr Frédéric Lambert
EDTA anticoagulated bone marrow (3 ml).

DNA PCR followed with Fragment analysis, direct sequencing confirmation for suspected mutant.

As described by Lin et al. Leukemia, 2006.
UZ Brussel - Molecular Hematology CEBPalpha mutation analysis Marleen Bakkus/Christian Demanet
At diagnosis or relapse: DNA test, PCR (TAD1, TAD2 and bZIP domain) and capillary electrphoresis. Multiplex assay with Flt3-ITD and NPM1 mutation analysis. Consensus primers from working group MolecularDiagnostics.be.
Version date: 26/09/2017
Accredited:
AZ St-Jan Brugge - Laboratoriumgeneeskunde CEBPalpha mutation analysis dr. Helena Devos/Friedel Nollet, Ph.D.
At diagnosis or relapse: DNA test, PCR (TAD1, TAD2 and bZIP domain) and capillary electrphoresis. Multiplex assay with Flt3-ITD and NPM1 mutation analysis. Consensus primers from working group MolecularDiagnostics.be. DNA sequencing of all three amplicons is performed when at least one insertion/deletion mutation is observed.
Version date: 04/11/2017
UZ Gent CEBPalpha mutation analysis Barbara Denys,
Karl Vandepoele
Simultaneous detection with FLT3-ITD and NPM1
DNA test

CEBPA is also present in our NGS panel for myeloid malignancies
Version date: 18/08/2017
Accredited:
UZ Leuven MolPLUZ CEBPalpha mutation analysis Lucienne Michaux / Els Lierman
Sample type: blood / bone marrow (EDTA, 2-5 ml)
Frequency: 1x/week
Test type: PCR on DNA, adapted from Benthaus T. et al, Br J Haematol 2008. Can also be performed on RNA.
Accredited:
CUB Erasme - Medical Genetics Dpt CEBPalpha mutation analysis Pierre Heimann/Barbara Dessars/Hakim El Housni
PCR and capillary electrophoresis

Analytical sensitivity : 5%
CHU Liège - Hemato CEBPalpha mutation analysis Dr Frédéric LAMBERT
Familial MDS/AML; Inherited predisposition syndrome to MDS/AML.
Screening could be performed on bone marrow BUT confirmation of germinal status request skin biopsy/fibroblasts grouwth.
Informed consent formulaire requested: http://www.chu.ulg.ac.be/jcms/c_9635594/informations-pratiques-relatives-aux-analyses
AZ Delta CEBPalpha mutation analysis Geert Martens, Elke Boone
SAMPLE TYPE: blood or bone marrow (1 ml)
FREQUENCY: upon request, at diagnosis or relapse
TEST TYPE: DNA test, PCR (TAD1, TAD2 and bZIP domain) and capillary electrophoresis. Multiplex assay with Flt3-ITD and NPM1 mutation analysis. Consensus primers from working group MolecularDiagnostics.be.
Version date: 05/12/2017
Accredited: