Digital droplet PCR with PrimePCR ddPCR mutation assay for p.D816V.
Sample type: Blood and bone marrow
BELAC accreditation number: 381-MED.
Accredited:
UZ Leuven MolPLUZ
KIT D816V mutation analysis
Jolien De Bie
Sample type: blood / bone marrow (EDTA, 2-5 ml)
Frequency: 1x/week
Test type: real-time PCR on DNA, based on Kristensen et al, Journal of molecular diagnostics 2011.
BELAC accreditation number 215-MED
Accredited:
LHUB-ULB
KIT D816V mutation analysis
Pierre Heimann/Laurent Dewispelaere/Pascale Cochaux/Hakim El Housni
SAMPLE TYPE: Peripheral blood (5mL) or bone marrow (EDTA, 1-2 mL)
TRANSPORT: room temperature, within 24h after sampling
FREQUENCY: 1x/2weeks; max TAT: 4 weeks
TEST TYPE: Real-Time PCR based on Kristensen et al. (Journal of Mol. Diagnostics 2011); analytical sensitivity: 0.2%
KIT is also analyzed in several in-house developed NGS panels designed to refine diagnosis and prognosis of AML, MDS, MPN and MDS/MPN overlap syndrome.
BELAC accreditation number 650-MED
Version date: 04/10/2021 Accredited:
UZA Mol. Diagn.
KIT D816V mutation analysis
K. Vermeulen,
M. Le Mercier,
M-B Maes
digital droplet PCR (ddPCR) on DNA with PrimePCR ddPCR Mutation Assay (Bio-Rad) for the p.D816V mutation in KIT. Sensitivity ≤ 0,01%.
Sample type: Blood and Bone marrow
BELAC accreditation number 204-MED
Version date: 06/09/2022 Accredited:
UZ Gent
KIT D816V mutation analysis
Karl Vandepoele
Annelynn Wallaert
Barbara Denys
real time PCR based on Kristensen et al. (Am J Hematol 2014)
Analysis performed on DNA.
FREQUENCY: 1x/week
BELAC accreditation number 087-MED
Version date: 31/05/2023 Accredited:
Sample type: Blood and bone marrow
BELAC accreditation number: 381-MED.
Accredited: