JAK2 V617F mutation analysis

JAK2 p.Val617Phe (c.1849G>T) mutation analysis

Lab	Test name	Contact	Comments
AZ St-Jan Brugge - Laboratoriumgeneeskunde	JAK2 V617F mutation analysis	dr. Helena Devos/Friedel Nollet, Ph.D.	LNA based real time PCR with WT-JAK2 blocking probe based on Denys et al., Journal of Molecular Diagnostics (2010) DNA-test Version date: 04/11/2017 Accredited:
UZA Mol. Diagn.	JAK2 V617F mutation analysis	K. Vermeulen, M. Le Mercier, M-B Maes	digital droplet PCR (ddPCR) on DNA with PrimePCR ddPCR Mutation Assay (Bio-Rad) for the p.(V617F) mutation in JAK2. Sample type: Blood and Bone marrow BELAC accreditation number 204-MED Version date: 06/09/2022 Accredited:
IPG Gosselies	JAK2 V617F mutation analysis	P. Vannuffel C. De Rop	Digital Droplet PCR (ddPCR - Biorad) with PrimePCR ddPCR mutation assay for the p.V617F mutation in JAK2. Sample type: Blood and bone marrow BELAC accreditation number: 381-MED Version date: 02/09/2022 Accredited:
UZ Gent	JAK2 V617F mutation analysis	Barbara Denys Karl Vandepoele	JAK2 c.1849G>T; p.Val617Phe Real-time PCR with LNA WT-JAK2 blocking probe Denys et al., J Mol Diagn 2010 DNA-test BELAC accreditation number 087-MED Version date: 16/03/2021 Accredited:
UZ Brussel - Molecular Hematology	JAK2 V617F mutation analysis	Marleen Bakkus/Barbara Depreter	c.1849G>T; p.Val617Phe Real-time PCR with LNA WT-JAK2 blocking probe Sensitivity: <0.1% Denys et al., J Mol Diagn 2010 DNA-test BELAC accreditation number: 141-MED Version date: 12/04/2023 Accredited:
CHU Liège - Hemato	JAK2 V617F mutation analysis	Dr Frédéric Lambert	As described by Sidon P et al., Clin Chem, 2006 and Denys B et al., J Mol Diagn. 2010 BELAC accreditation number: 128-MED Accredited:
Jessa Hospital	JAK2 V617F mutation analysis	Brigitte Maes/Ellen Geerdens	SAMPLE TYPE: Blood or bone marrow (1ml, EDTA) TRANSPORT: room temperature, within 24h after sampling FREQUENCY: 1x/week TEST TYPE: Real-time RT-PCR, allelic discrimination, in house method BELAC Accreditation number 275-MED Version date: 25/05/2021 Accredited:
LHUB-ULB	JAK2 V617F mutation analysis	Pierre Heimann/Laurent Dewispelaere/Pascale Cochaux/Hakim El Housni	SAMPLE TYPE: Peripheral blood or bone marrow (EDTA, 1-2 mL) TRANSPORT: room temperature, within 24h after sampling FREQUENCY:variable; max TAT: 4 weeks TEST TYPE: Real-Time PCR with LNA WT-Jak2 blocking probe (Sidon et al. Clin. Chem. 2006) The exon 14 of JAK2 is also analyzed in several in-house developed NGS panels designed to refine diagnosis and prognosis of MPN and MDS/MPN overlap syndrome. BELAC accreditation number 650-MED Version date: 04/10/2021 Accredited:
GZA St-Augustinus	JAK2 V617F mutation analysis	Philippe Van Lint	real time PCR with LNA WT-JAK2 blocking probe based on Sidon et al., Clin Chem (2006) with modifications DNA-test performed on whole blood, or bone marrow (EDTA) frequency: 1x/week BELAC accreditation number 404-MED Accredited:
OLVZ Aalst	JAK2 V617F mutation analysis	Anne Vankeerberghen	Sample type: EDTA-Blood or bone marrow (500 µl) Frequency: Weekly Test type: In house real time PCR Version date: 06/05/2019 Accredited:
AZ St-Lucas Gent	JAK2 V617F mutation analysis	Dr H. Louagie / Koen Jacobs	SAMPLE TYPE: Blood or bone marrow FREQUENCY: 1x/2weeks TEST TYPE: JAK2 V617F mutaquant kit (Ipsogen)
AZ Delta	JAK2 V617F mutation analysis	Elisabeth Moreau/Elke Boone	SAMPLE TYPE: Blood or bone marrow (1ml, EDTA) FREQUENCY: 1x/2-weeks TEST TYPE: RT-PCR followed by RFLP, in house method Version date: 05/12/2017 Accredited:
ZNA Middelheim	JAK2 V617F mutation analysis	Pieter De Schouwer	Sample type: bone marrow or EDTA blood Frequency: once a week test type: in house PCR
Labo Cliniques Sud Luxembourg	JAK2 V617F mutation analysis	Nicolas Hougardy	SAMPLE TYPE: - Blood (EDTA) (min 200µL) - Bone Marrow (EDTA) (min 2,5ml) FREQUENCY: every two weeks TEST TYPE : In-House, Real time PCR (Screening of acquired abnormalities in the diagnosis of a CML and for the follow-up of a myeloid affection), LightCycler 2.0 (Roche) ARTICLES: "Lay Jmol Diag, 2006, 8, n°3, 330-334" and "Mac Clure leukemia, 2006,168-171"
UZ Leuven MolPLUZ	JAK2 V617F mutation analysis	Jolien De Bie	Sample type: blood / bone marrow (EDTA, 2-5 ml) Frequency: 1x/week Test type: digital droplet PCR (ddPCR) on DNA with PrimePCR ddPCR Mutation Assay (Bio-Rad) for the p.V617F mutation in JAK2. BELAC accreditation number 215-MED Accredited:
AZ Damiaan Oostende	JAK2 V617F mutation analysis	Ben Vanmassenhove/ Anne-Sophie Hervent	Protocol: B. Denys et al (2010) BELAC accreditation number 433-MED Version date: 11/08/2023 Accredited: