NPM1 mutation analysis

NPM1 (nucleophosmin) mutation analysis
Lab Test name Contact Comments
AZ St-Jan Brugge - Laboratoriumgeneeskunde NPM1 mutation analysis dr. Helena Devos/Friedel Nollet, Ph.D.
Only at diagnosis or relapse: DNA test, PCR and capillary electrophoresis. Multiplex assay with Flt3-ITD and CEBPalpha mutation analysis. Consensus primers from working group MolecularDiagnostics.be. Sanger DNA-sequencing is used to detect type of mutation.
Version date: 04/11/2017
Accredited:
IPG Gosselies NPM1 mutation analysis
UZ Gent NPM1 mutation analysis Barbara Denys,
Karl Vandepoele
Simultaneous detection with FLT3-ITD and CEBPA (capillary electrophoresis)
DNA test

NPM1 is also present in our NGS panel for myeloid malignancies.
Version date: 18/08/2017
Accredited:
CHU Liège - Hemato NPM1 mutation analysis Dr Frédéric Lambert
As described by Scholl et al., Leuk Research, 2007 or
QIAGEN (ipsogen) NPM1 MutaScreen Kit
Jessa Hospital NPM1 mutation analysis Dr. Brigitte Maes, Femke Hillen
SAMPLE TYPE:
Blood or bone marrow (1ml, EDTA)
TRANSPORT: room temperature
FREQUENCY: 1x/week
TEST TYPE: Only at diagnosis or relapse: DNA test, PCR and capillary electrophoresis. Multiplex assay with Flt3-ITD mutation analysis. Consensus primers from working group MolecularDiagnostics.be.
UZ Leuven MolPLUZ NPM1 mutation analysis Lucienne Michaux / Els Lierman
Sample type: blood / bone marrow (EDTA, 2-5 ml)
Frequency: 2x/week
Test type: PCR on cDNA, adapted from Noguera et al, Leukemia 2002.
Accredited:
CUB Erasme - Medical Genetics Dpt NPM1 mutation analysis Pierre Heimann/Barbara Dessars/Hakim El Housni
PCR and capillary electrophoresis

Analytical sensitivity: 1%
UZ Brussel - Molecular Hematology NPM1 mutation analysis Marleen Bakkus/Christian Demanet
Only at diagnosis or relapse: DNA test, PCR and capillary electrphoresis. Multiplex assay with Flt3-ITD and CEBPalpha mutation analysis. Consensus primers from working group MolecularDiagnostics.be.
Version date: 26/09/2017
Accredited:
AZ Delta NPM1 mutation analysis Geert Martens, Elke Boone
SAMPLE TYPE: blood or bone marrow (1 ml)
FREQUENCY: upon request, at diagnosis or relapse
TEST TYPE: DNA test, PCR and capillary electrophoresis. Multiplex assay with CEBPA and FLT3-ITD mutation analysis. Consensus primers from working group MolecularDiagnostics.be.
Version date: 05/12/2017
Accredited: