Only at diagnosis or relapse: DNA test, PCR and capillary electrophoresis. Multiplex assay with Flt3-ITD and CEBPalpha mutation analysis. Consensus primers from working group MolecularDiagnostics.be. Sanger DNA-sequencing is used to detect type of mutation.
Version date: 04/11/2017 Accredited:
IPG Gosselies
NPM1 mutation analysis
P. Vannuffel
C. De Rop
Multiplex assay with FLT3-ITD mutation analysis. Consensus primers from working group MolecularDiagnostics.be.
Capillary electrophoresis.
Sample type: Blood and bone marrow.
Accredited:
UZ Gent
NPM1 mutation analysis
Barbara Denys,
Karl Vandepoele
Simultaneous detection with FLT3-ITD, FLT3-TKD and CEBPA (capillary electrophoresis)
DNA test
BELAC accreditation number 087-MED
NPM1 is also present in our NGS panel for myeloid malignancies.
Version date: 16/03/2021 Accredited:
Jessa Hospital
NPM1 mutation analysis
Dr. Brigitte Maes, Ellen Geerdens
SAMPLE TYPE:
Blood or bone marrow (1ml, EDTA)
TRANSPORT: room temperature
FREQUENCY: 1x/week
TEST TYPE: Only at diagnosis or relapse: DNA test, PCR and capillary electrophoresis. Multiplex assay with Flt3-ITD mutation analysis. Consensus primers from working group MolecularDiagnostics.be.
UZ Leuven MolPLUZ
NPM1 mutation analysis
Jolien De Bie
Sample type: blood / bone marrow (EDTA, 2-5 ml)
Frequency: 1x/week
Test type: PCR on cDNA, adapted from Noguera et al, Leukemia 2002.
BELAC accreditation number 215-MED
Accredited:
LHUB-ULB
NPM1 mutation analysis
Pierre Heimann/Laurent Dewispelaere/Pascale Cochaux/Hakim El Housni
SAMPLE TYPE: Peripheral blood (5mL) or bone marrow (EDTA, 1-2 mL)
TRANSPORT: room temperature, within 24h after sampling
FREQUENCY: upon request; max TAT: 4 weeks
TEST TYPE: PCR and capillary eletrophoresis (consensus primers from MD.be)
NPM1 is also analyzed in several in-house developed NGS panels designed to refine diagnosis and prognosis of AML, MDS, MPN and MDS/MPN overlap syndrome.
BELAC accreditation number 650-MED
Version date: 04/10/2021 Accredited:
UZ Brussel - Molecular Hematology
NPM1 mutation analysis
Marleen Bakkus/Barbara Depreter
Sample type: EDTA bone marrow (1 ml) or EDTA blood (5ml).
Transport: room temperature, within 72h after sampling
Frequency: 1x/week
Only at diagnosis or relapse: DNA test, PCR and capillary electrophoresis. Multiplex assay with Flt3-ITD and CEBPalpha mutation analysis. Consensus primers from working group MolecularDiagnostics.be.
BELAC accreditation number: 141-MED
Version date: 12/04/2023 Accredited:
AZ Delta
NPM1 mutation analysis
Geert Martens, Elke Boone
SAMPLE TYPE: blood or bone marrow (1 ml)
FREQUENCY: upon request, at diagnosis or relapse
TEST TYPE: DNA test, PCR and capillary electrophoresis. Multiplex assay with CEBPA and FLT3-ITD mutation analysis. Consensus primers from working group MolecularDiagnostics.be.
Version date: 05/12/2017 Accredited:
Version date: 04/11/2017
Accredited: