IgH clonality analysis

Detection of IgH monoclonality
Lab Test name Contact Comments
AZ St-Jan Brugge - Laboratoriumgeneeskunde IgH clonality analysis dr. Helena Devos/Friedel Nollet, Ph.D.
PCR on DNA followed by capillary electrophoresis.
FR1-JH and FR3-JH primers from BIOMED-2 consortium, Van Dongen et al. Leukemia (2003)
Version date: 04/11/2017
Accredited:
UZA Mol. Diagn. IgH clonality analysis K. Vermeulen, M-B Maes
IPG Gosselies IgH clonality analysis
UZ Gent IgH clonality analysis Barbara Denys
Karl Vandepoele
BIOMED-2 protocol according to van Dongen et al, Leukemia 2003;
DNA-test
Version date: 11/10/2017
Accredited:
UZ Brussel - Molecular Hematology IgH clonality analysis Marleen Bakkus/Christian Demanet
Fluorescent PCR on DNA followed by Fragment analysis, in-house and Biomed-2 protocol targets FR1, FR2, FR3 and DJ (van Dongen et al,Leukemia, 2003)
Version date: 26/09/2017
Accredited:
CHU Liège - Hemato IgH clonality analysis Dr Sc Sabine Franke
Commercial assay: IdentitiClone IgH Gene Clonality Assay, InVivoScribe Technologies.
Jessa Hospital IgH clonality analysis Dr. Brigitte Maes, Femke Hillen
SAMPLE TYPE:
-Blood or bone marrow (1ml, EDTA)
-Biopsy (fresh or FFPE)
-CSF (or other fluids, 1ml)
TRANSPORT: room temperature
FREQUENCY:1x/week
TEST TYPE:PCR & Fragment analysis, in house method from van Dongen et al,Leukemia, 2003, Biomed-2
CUB Erasme - Medical Genetics Dpt IgH clonality analysis Pierre Heimann/Barbara Dessars/Hakim El Housni
Fluorescent PCR followed by size fragment analysis
Kit from Invivoscribe company
OLVZ Aalst IgH clonality analysis Freya Vaeyens
Sample type:
EDTA-Blood or bone marrow (500 µl)
Gland tissue
Frequency: 1x in 2 weeks
Test type: Fragment analysis (Biomed primers)
Version date: 29/09/2017
Accredited:
ZNA Middelheim IgH clonality analysis Evelien Heylen
SAMPLE TYPE:
-Blood or bone marrow (1ml, EDTA)
-Biopsy (fresh or FFPE)
-CSF (or other fluids, 1ml)

FREQUENCY: 2x/week
TEST TYPE: Biomed-2 PCR according to van Dongen et al, Leukemia (2003)

Performed in conjunction with IgK and IgL clonality analysis
AZ Delta IgH clonality analysis Elisabeth Moreau/Elke Boone
SAMPLE TYPE:
-Blood or bone marrow (1ml, EDTA)
-Biopsy (fresh or FFPE)
-CSF (or other fluids, 1ml)
FREQUENCY:1x/week
TEST TYPE: PCR & Fragment analysis, Biomed-2 protocol targets FR1, FR2, FR3 and DJ (van Dongen et al,Leukemia, 2003)
Version date: 05/12/2017
Accredited:
Labo Cliniques Sud Luxembourg IgH clonality analysis Nicolas Hougardy
SAMPLE TYPE:
- Blood (EDTA) (min 200µL)
- Bone marrow (EDTA) (min 2,5ml)
FREQUENCY: 1x/month
TEST TYPE: “ Identiclone IGH gene clonality assay”(Invivoscribe), Experion Electrophoresis System (BioRad)
UZ Leuven MolPLUZ IgH clonality analysis Lucienne Michaux / Els Lierman
Sample type: blood / bone marrow (EDTA, 2-5 ml) / tissue (fresh or FFPE)
Frequency: 1x/week
Test type: PCR on DNA, based on van Dongen et al., Leukemia 2003 (BIOMED-2 protocol).
Accredited: