WT1 overexpression analysis

Lab Test name Contact Comments
UZA Mol. Diagn. WT1 overexpression analysis K.Vermeulen, M-B Maes
Real-time kwantitatieve PCR met de WT1 mRNA OneStep assay (Otsuka Pharmaceutical) Lightcycler 480
Version date: 05/12/2017
Accredited:
UZ Gent WT1 overexpression analysis Barbara Denys,
Karl Vandepoele
Real-time PCR according to ELN guidelines (Cilloni et al., J Clin Oncol 2009)
RNA-test
Version date: 11/10/2017
Accredited:
CHU Liège - Hemato WT1 overexpression analysis Dr Frédéric Lambert
QIAGEN, ipsogen WT1 ProfileQuant Kit CE
CUB Erasme - Medical Genetics Dpt WT1 overexpression analysis Pierre Heimann/Barbara Dessars/Hakim El Housni
Home-made assay.
Test performed exclusively on peripheral blood
IPG Gosselies WT1 overexpression analysis
AZ Delta WT1 overexpression analysis Elisabeth Moreau/Elke Boone
SAMPLE TYPE: Blood or bone marrow (1ml, EDTA)
FREQUENCY:1x/2-weeks
TEST TYPE: real-time PCR in house protocol (Taqman gene expression assay for WT1 (exon 6/7) and ABL).
Version date: 05/12/2017
Accredited:
ZNA Middelheim WT1 overexpression analysis Pieter De Schouwer
In house method, normalised copy numbers / ABL.
Both Peripheral blood and bone marrow.
Lab specific reference values.
UZ Leuven MolPLUZ WT1 overexpression analysis Lucienne Michaux / Els Lierman
Sample type: blood / bone marrow (EDTA, 2-5 ml)
Frequency: 2x/week
Test type: real time PCR on cDNA (WT1 ProfileQuant Kit, Ipsogen)