BCL2/IgH qualitative analysis

detection of t(14;18) BCL2/IgH rearrangement
Lab Test name Contact Comments
AZ St-Jan Brugge - Laboratoriumgeneeskunde BCL2/IgH qualitative analysis dr. Helena Devos/Friedel Nollet, Ph.D.
- PCR on DNA for MBR, 3MBR and mcr breakpoints
Primers for BIOMED-2 consortium, Van Dongen et al. Leukemia (2003)
- FISH (contact: Barbara Cauwelier)
Version date: 04/11/2017
Accredited:
IPG Gosselies BCL2/IgH qualitative analysis P. Vannuffel
C. De Rop
In house method based on Oscar et al, Mod Patholo, 2000.
RT-PCR.
Sample type: Blood and bone marrow.
BELAC accreditation number: 381-MED.
Version date: 02/09/2022
Accredited:
UZ Gent BCL2/IgH qualitative analysis Barbara Denys
Karl Vandepoele
BIOMED-2 protocol according to van Dongen et al, Leukemia 2003;
DNA-test
BELAC accreditation number 087-MED
Version date: 16/03/2021
Accredited:
UZ Brussel - Molecular Hematology BCL2/IgH qualitative analysis Marleen Bakkus/Barbara Depreter
PCR on DNA from bone marrow, peripheral blood or biopsies.
Sensitivity: 1E-04
BELAC accreditation number: 141-MED
Version date: 12/04/2023
Accredited:
UZ Gent - CMGG BCL2/IgH qualitative analysis nadine van roy
ZNA Middelheim BCL2/IgH qualitative analysis Evelien Heylen
SAMPLE TYPE:
-Blood or bone marrow (1ml, EDTA)
-Biopsy (fresh or FFPE)
-CSF (or other fluids, 1ml)

FREQUENCY: 2x/week
TEST TYPE: in house real-time PCR detecting MBR and MCR breakpoints
AZ Delta BCL2/IgH qualitative analysis Elisabeth Moreau/Elke Boone
SAMPLE TYPE:
-Blood or bone marrow (1ml, EDTA)
-Biopsy (fresh or FFPE)
-CSF (or other fluids, 1ml)
FREQUENCY:1x/week
TEST TYPE: PCR, Biomed-2 protocol targets MBR, mcr and 3MBR (van Dongen et al,Leukemia, 2003)
Version date: 05/12/2017
Accredited:
Labo Cliniques Sud Luxembourg BCL2/IgH qualitative analysis Nicolas Hougardy
SAMPLE TYPE:
- Blood (EDTA) (min 200µL)
- Bone marrow (EDTA) (min 2,5ml)
FREQUENCY: 1x/month
TEST TYPE: “ Identiclone BCL2/JH Translocation Assay ”(Invivoscribe), Experion Electrophoresis System (BioRad)