Toxoplasma

Molecular detection of Toxoplasma gondii
Lab Test name Contact Comments
UZ Brussel - Microbiology Toxoplasma Dr. Denis Piérard, Oriane Soetens
SAMPLE TYPE:
-amniotic fluid (min. 2 ml)
-CSF (min. 0.2 ml)
-ocular fluid (min. 0.2 ml)
-for other sample types previously contact laboratory
FREQUENCY: every weekday
TEST TYPE: in-house real-time PCR
TARGETS: B1 gene and AF146527
Version date: 26/09/2017
Accredited:
UZA Mol. Diagn. Toxoplasma D. Ursi, K. Bergs, M. Michiels, V. Matheeussen
Sample type:
- ocular fluid or swab
- cerebrospinal fluid
(at least 250 µl).
- amniotic fluid (at least 1 ml)

Frequency: 2 times a week

Test type: in house real-time PCR
UZ Leuven MolPLUZ Toxoplasma Katrien Lagrou
Sample type:
- EDTA-blood
- CSV
- BAL
- amniotic fluid
- ocular fluid
- biopsy

Minimal sample volume: 1ml EDTA blood / amniotic fluid - 250 µl all other samples
Frequency: 2-5 times a week
Test type: Quantitative in-house realtime PCR
CHU Saint-Pierre Toxoplasma Reynders Marijke, Maaroufi Younes, Scheen Robert
Staaltypes: EDTA-bloedstalen, oogvochten, CSV, amnionvocht, placenta, biopten.


Minimum staalvolume: 250 microliter


Kwalitatieve in house real-time PCR
ZNA Middelheim Toxoplasma Walter Verstrepen
Sample type:
- ocular fluid or swab
- cerebrospinal fluid
(at least 250 µl).
- amniotic fluid (at least 1 ml)
-EDTA whole blood (at least 500 µl)

Frequency: every working day
Test type: in house real-time PCR
Labo Cliniques Sud Luxembourg Toxoplasma Nicolas Hougardy
SAMPLE TYPE:
- Plasma (EDTA)(min 1ml)
FREQUENCY: 1x/week
TEST TYPE: in-house, real time PCR (18S RNA detection), LightCycler 2.0 (Roche)
Jessa Hospital Toxoplasma dr Cartuyvels, Raymaekers Marijke
SAMPLE TYPE:
-whole blood
FREQUENCY: 1x/week
TEST TYPE: in house real-time PCR
Accredited:
Jessa Hospital Toxoplasma dr Cartuyvels, Raymaekers Marijke
SAMPLE TYPE:
-CSV
-amniotic fluid
FREQUENCY: from Monday till Friday
TEST TYPE: in house real-time PCR
Accredited:
AZ St-Jan Brugge - Laboratoriumgeneeskunde Toxoplasma Dr. M. Reynders / P. Descheemaeker
SAMPLE TYPE:
- Whole blood
- Amniotic fluid
- CSF
FREQUENCY: 1x/week (Friday/Monday)
TEST TYPE: in-house real time PCR
TARGETS: B1 gene and AF146527
Version date: 17/11/2017
Accredited: