hematology - lymfoid gene panels

Lab	Test name	Contact	Comments
UZA Mol. Diagn.	hematology - lymfoid gene panels	K. Vermeulen, M. Le Mercier, M-B Maes	60 genes Haloplex HS NGS panel on MiSeq to screen clinically relevant genes in lymphoid malignancies (incl. CLL, DLBCL, LP, etc). Genes: ARID1A, ATM, B2M, BCL2, BIRC3, BRAF, BTK, CARD11, CD28, CD58, CD79A, CD79B, CDKN2A, CREBBP, CXCR4, DNMT3A, DDX3X, EP300, EZH2, FBXW7, FGFR1, FGFR2, FGFR3, FOXO1, GNA13, ID3, IDH1, IDH2, IKZF1, IRF4, JAK1, JAK2, JAK3, KMT2D/MLL2, KRAS, MAP2K1, MEF2B, MYC, MYD88, NFKBIE, NOTCH1, NOTCH2, NRAS, PDGFRA, PIM1, PLCG2, PTEN, RHOA, SF3B1, SOCS1, STAT3, STAT5B, STAT6, TCF3, TET2, TNFAIP3, TNFRSF14, TP53, TRAF2, XPO1. Sample Type: Blood, Bone marrow, Biopt, FFPE samples BELAC accreditation number 204-MED Version date: 09/09/2022 Accredited:
CHU Liège - Hemato	hematology - lymfoid gene panels	Dr Frédéric LAMBERT, Benjamin KOOPMANSCH & Rafael Carazo	Inherited Syndromes Predisposing to Adult-Onset Hematologic Malignancy: Familial B-cell ALL with PAX5; Germline SH2B3; Li-Fraumeni syndrome with ALL or therapy-related AML (TP53) NGS targeted sequencing after hybridization capture, Home brew assay. Skin biopsy requested or fibroblasts'DNA. INFORMED CONSENT REQUESTED BEFORE TESTING: http://www.chu.ulg.ac.be/jcms/c_10919499/formulaire-de-consentement-ngs BELAC accreditation number: 128-MED Accredited:
UZ Leuven MolPLUZ	hematology - lymfoid gene panels	Jolien De Bie	Sample type: blood / bone marrow (EDTA, 2-5 ml) Frequency: 1x/week Test type: targeted capture NGS workflow with custom IDT xGen® Lockdown® Probes on an Illumina sequencing instrument Genes: ACTG1, ANKRD26, ATM, BCOR, BCORL1, BIRC3, BRAF, BTK, CARD11, CREBBP, CRLF2, CXCR4, CYLD, DDX41, DIS3, DNMT3A, EBF1, EGR1, ELANE, ETV6, EZH2, FAM46C, FBXW7, FGFR3, GATA2, GFI1, HRAS, IKZF1, IL7R, IRF4, JAK1, JAK3, KRAS, MYD88, NOTCH1, NRAS, PHF6, PLCG2, POT1, PPM1D, PRDM1, PRKD2, PTEN, PTPN11, RAD21, RB1, SAMD9, SAMD9L, SETD2, SF3B1, SMC1A, SMC3, SP140, SRP72, STAG1, STAG2, STAT5B, TP53, TRAF3, WAS, XPO1 BELAC accreditation number 215-MED Accredited:
Jessa Hospital	hematology - lymfoid gene panels	Dr. Brigitte Maes, Guy Froyen	AmpliSeq myeloid panel (Illumina) (DNA + RNA) Genes: ABL1, ASXL1, ATRX, BCOR, BCORL1, BRAF, CALR, CBL, CBLB, CBLC, CDKN2A, CEBPA, CSF3R, CUX1, DNMT3A, ETV6, EZH2, FBXW7, FLT3, GATA1, GATA2, GNAS, HRAS, IDH1, IDH2, IKZF1, JAK2, JAK3, KDM6A, KIT, KRAS, KMT2A, MPL, MYD88, NOTCH1, NPM1, NRAS, PDGFRA, PHF6, PTEN, PTPN11, RAD21, RUNX1, SETBP1, SF3B1, SMC1A, SMC3, SRSF2, STAG2, TET2, TP53, U2AF1, WT1, ZRSR2 2x/week on MiSeq Accredited 275-MED Version date: 03/08/2021 Accredited:
AZ Delta	hematology - lymfoid gene panels	Geert Martens, Elke Boone	Sample type: blood / bone marrow (300µl - 1 ml) Frequency: 1x/month Test type: TruSight Myeloid (Illumina), NGS Genes: BRAF, FBXW7, IKZF1, MYD88, NOTCH1, PTEN, SF3B1, TP53, ... Version date: 05/12/2017 Accredited:
UZ Gent	hematology - lymfoid gene panels	Karl Vandepoele, Annelynn Wallaert Joni Van Der Meulen, Barbara Denys	In-house developed, capture-based (HyperCap) 116 gene panel to screen clinically relevant genes in hematological disorders by NGS: ANKRD26, ARID1A, ASXL1, ATM, B2M, BCL2, BCOR, BCORL1, BIRC3, BRAF, BTG1, BTK, CALR, CARD11, CBL, CBLB, CCL22, CCND1, CD28, CD33, CD58, CD79A, CD79B, CDKN2A, CEBPA, CREBBP, CSF3R, CUX1, CXCR4, DDX41, DDX3X, DIS3, DNMT3A, EGR2, EP300, ETNK1, ETV6, EZH2, FAS, FBXW7, FLT3, FOXO1, GATA1, GATA2, GNA13, GNB1, HAVCR2, HRAS, ID3, IDH1, IDH2, IL7R, IRF4, JAK1, JAK2, JAK3, KIT, KLF2, KMT2A, KMT2D, KRAS, MAP2K1, MEF2B, MPL, MYD88, NF1, NFE2, NFKBIE, NPM1, NOTCH1, NOTCH2, NRAS, NSD2, NUDT15, PHF6, PIK3CA, PIM1, PLCG1, PLCG2, POT1, PPM1D, PRKCB, PRPF8, PTEN, PTPN11, RAD21, RHOA, RUNX1, SBDS, SETBP1, SF3B1, SGK1, SH2B3, SMC1A, SMC3, SOCS1, SRSF2, STAG2, STAT3, STAT5B, STAT6, TCF3, TET2, TNFAIP3, TNFRSF14, TP53, TPMT, TRAF2, U2AF1, UBA1, UBTF, XPO1, WT1, ZEB2, ZRSR2 1x/week BELAC accreditation number 087-MED Version date: 31/05/2023 Accredited:
UZ Brussel - Molecular Pathology	hematology - lymfoid gene panels	Freya Vaeyens	NGS targeted sequencing after hybridization capture, home brew STHT assay (solid and hematological tumors) Genes list available at http://www.brightcore.be/stht Sample Type: blood/ bone marrow Frequency: 1x/week Accreditation: BELAC 141-MED Version date: 10/10/2019 Accredited:
UZ Brussel - Molecular Pathology	hematology - lymfoid gene panels	Freya Vaeyens	Fusion NGS panel - TSO500 Illumina Sample Type: blood / bone marrow Frequency: 1x/week Version date: 10/02/2021
LHUB-ULB	hematology - lymfoid gene panels	Pierre Heimann/Laurent Dewispelaere/Pascale Cochaux/Hakim El Housni	TRANSPORT: room temperature, within 24h after sampling FREQUENCY: 1x/week; max TAT: 4 weeks TEST TYPE: In-house developed gene panel using the Qiaseq Targeted DNA custom Panels platform (Qiagen) to screen for variants in lymphoid diseases: BRAF, BTK, CXCR4, KRAS, MYD88, NRAS, SF3B1, TP53 BELAC accreditation number 650-MED Version date: 04/10/2021 Accredited:
LHUB-ULB	hematology - lymfoid gene panels	Pierre Heimann/Laurent Dewispelaere/Pascale Cochaux/Hakim El Housni	TRANSPORT: room temperature, within 24h after sampling FREQUENCY: 1x/week; max TAT: 4 weeks TEST TYPE: In-house developed gene panel using the Qiaseq Targeted DNA custom Panels platform (Qiagen) to screen for variants in acute T-cell leukemia: FBXW7, HRAS, KRAS, NOTCH1, NRAS, PTEN, TP53 BELAC accreditation number 650-MED Version date: 04/10/2021