solid tumors - gene panels

Lab	Test name	Contact	Comments
AZ Delta	solid tumors - gene panels	Geert Martens/Franceska Dedeurwaerdere/Joke Breyne	In-house NGS panel (SeqCap EZ Choice custom design, KAPA Hyperplus workflow on Miseq) for the detection of mutations, indels, CNV in the following genes: Lung panel (ALK,BRAF, CDKN2A, DDR2,EGFR,ERBB2,KRAS,MET,PIK3CA,RET,ROS1,TP53) Colon panel (BRAF,KRAS,NRAS,PIK3CA,TP53) Melanoma panel (BRAF,KIT,NRAS) GIST panel (BRAF,KIT,PDGFRA) BRAIN panel (BRAF,H3F3A,IDH1/2) BREAST/OVARIAN panel (BRCA1/2,ERBB2,ESR1,PIK3CA,TP53) Other genes tested but not standard reported: AKT1,APC,ARAF,CTNNB1,ERBB4,GNAS,HRAS,MAP2K1,NF1,NRG1,NTRK1,PIK3R1,PTEN,SMAD4,TERT Version date: 05/12/2017 Accredited:
UZ Gent	solid tumors - gene panels	Joni Van der Meulen Siebe Loontiens Suzanne Vanhauwaert	Sample Type: FFPE Tissue Frequency: 1x/week SOLID MDG DNA NGS panel for detection of SNV and indels (112 genes, KAPA HyperCap Roche): AKT1, ALK, APC, AR, ARID1A, ATM, BAP1, BARD1, BCOR, BRAF, BRCA1, BRCA2, BRIP1, CCND1, CCNE1, CDH1, CDK4, CDK6, CDK12, CDKN2A, CDKN2B, CHEK1, CHEK2, CTNNB1, DDX3X, DGCR8, DICER1, DPYD, DROSHA, EGFR, EIF1AX, ELP1, ERBB2, ERBB3, ESR1, FBXW7, FGFR1, FGFR2, FGFR3, FGFR4, FOXL2, FRK, GATA3, GNA11, GNAQ, GNAS, GPR161, H3-3A, H3-3B, H3C2, H3C3, HNF1A, HRAS, IDH1, IDH2, IL6ST, JAK1, JAK2, KBTBD4, KEAP1, KIT, KRAS, MAP2K1, MDM2, MET, MRE11, MTAP, MYC, MYCN, MYOD1, NBN, NF1, NF2, NOTCH1, NRAS, NTRK1, NTRK2, NTRK3, PALB2, PDGFRA, PDGFRB, PIK3CA, PIK3R1, POLE, PRKCA, PTCH1, PTEN, PTPN11, RAD50, RAD51B, RAD51C, RAD51D, RB1, RET, RNF43, ROS1, SDHA, SDHB, SDHC, SDHD, SF3B1, SMAD4, SMARCA4, SMARCB1, SMO, SPOP, STAT3, STK11, SUFU, TERT, TP53, VHL BELAC accreditation number (351-MED) Version date: 01/07/2023 Accredited:
UZ Gent	solid tumors - gene panels	Joni Van der Meulen Siebe Loontiens Suzanne Vanhauwaert	Sample Type: FFPE Tissue Frequency: 1x/week SOLID MDG RNA NGS panel for detection of fusions and splice variants (26 genes, Archer FusionPlex): ALK, BRAF, EGFR, ERG, FGFR1, FGFR2, FGFR3, KRAS, MET, MYB, MYBL1, MYC, NRG1, NTRK1, NTRK2, NTRK3, PPARG, PRKCA, RAF1, RELA, RET, ROS1, RSPO2, RSPO3, TMPRSS2, YAP1 BELAC accreditation number (351-MED) Version date: 16/03/2021 Accredited:
CHU Liège - Hemato	solid tumors - gene panels	Dr Frédéric LAMBERT, Benjamin Koopmansch, Rafael Carazo	Amplicon sequencing: Tumor Hot Spot Master Plus, Agilent (Multiplicon). Genes list available at: http://www.chu.ulg.ac.be/jcms/c_9635594/informations-pratiques-relatives-aux-analyses As suggested by Sciensano / ComPermed: https://www.compermed.be/fr/workflows BELAC accreditation number: 128-MED Accredited:
UZ Leuven MolPLUZ	solid tumors - gene panels	Isabelle Vanden Bempt Sara Vander Borght, Lien Spans, Sofie Claerhout	Sample type: FFPE / fresh tissues Frequency: 2x/ week Test type: KAPA Hyperplus NGS workflow with custom IDT xGen® Lockdown® Probes on an Illumina sequencing instrument. Goal: detection of somatic variants and indels Genes: AKT1, ALK, AMER1, APC, ARAF, ARID1A, ATRX, BAP1, BCOR, BRAF, BRCA1, BRCA2, CCND1, CCNE1, CDH1, CDK4, CDK6, CDKN2A, CIC, CTNNB1, DDR2, DICER1, EGFR, ERBB2, ERBB3, ERBB4, ERCC2, ESR1, FBXW7, FGFR1, FGFR2, FGFR3, FGFR4, FOXL2, FUBP1, GATA3, GLI1, GLI2, GNA11, GNAQ, GNAS, H3-3A, H3-3B, H3C2, H3C3, HRAS, IDH1, IDH2, KIT, KRAS, LZTR1, MAP2K1, MDM2, MDM4, MET, mTOR, MYC, MYCN, NF1, NF2, NOTCH1, NRAS, PALB2, PDGFRA, PDGFRB, PIK3CA, PIK3R1, POLE, PRDM6, PTCH1, PTEN, RAC1, RAF1, RB1, RET, RICTOR, RNF43, ROS1, SDHA, SDHB, SDHC, SDHD, SMAD4, SMARCA4, SMARCB1, SMO, SPRED1, STK11, SUFU, TERT, TP53, TSC1, TSC2, VEGFR3 (FLT4), WT1. Depending on the indication, a subset of the captured regions is examined (see website for more details). BELAC accreditation number: 215-MED Version date: 20/12/2022 Accredited:
ULB-Erasme Pathology	solid tumors - gene panels	Nicky D’Haene	Sample Type: FFPE Tissue Frequency: 1x/week Test Type : Lung Fusion NGS panel (ALK, ROS1, RET and NTRK1 rearrangements) on Ion Torrent PGM (ThermoFisher) Version date: 11/10/2017 Accredited:
ULB-Erasme Pathology	solid tumors - gene panels	Nicky D’Haene	Sample Type: FFPE Tissue Frequency: 1x/week Test Type : Colon & Lung NGS panel (22 genes : AKT1, ALK, BRAF, CTNNB1, DDR2, EGFR, ERBB2, ERBB4, FBXW7, FGFR1, FGFR2, FGFR3, KRAS, MAP2K1, MET, NOTCH1, NRAS, PIK3CA, PTEN, SMAD4, STK11, TP53) on Ion Torrent PGM (ThermoFisher) Version date: 11/10/2017 Accredited:
ULB-Erasme Pathology	solid tumors - gene panels	Nicky D’Haene	Sample Type: FFPE Tissue Frequency: 1x/week Test Type : NGS Cancer Panel (50 genes : ABL1, AKT1, ALK, APC, ATM, BRAF, CDH1, CDKN2A, CSF1R, CTNNB1, EGFR, ERBB2, ERBB4, EZH2, FBXW7, FGFR1, FGFR2, FGFR3, FLT3, GNA11, GNAQ, GNAS, HNF1A, HRAS, IDH1, IDH2, JAK2, JAK3, KDR, KIT, KRAS, MET, MLH1, MPL, NOTCH1, NPM1, NRAS, PDGFRA, PIK3CA, PTEN, PTPN11, RB1, RET, SMAD4, SMARCB1, SMO, SRC, STK11, TP53, VHL) on Ion Torrent PGM (ThermFisher) Version date: 11/10/2017 Accredited:
ULB-Erasme Pathology	solid tumors - gene panels	Nicky D’Haene	Sample Type: FFPE Tissue Frequency: 1x/week Test Type : NGS Gyneco Panel (16 genes : AKT1, BRAF, CDKN2A, CTNNB1, DICER1, ERBB2, FBXW7, FGFR2, FOXL2, KRAS, PIK3CA, PIK3R1, POLE, PTEN, RB1, TP53) on Ion Torrent PGM (ThermoFisher) Version date: 11/10/2017 Accredited:
Jessa Hospital	solid tumors - gene panels	Dr. Brigitte Maes, Guy Froyen	AmpliSeq Focus + custom panel (DNA + RNA) AmpliSeq BRCA panel 2x/week on MiSeq Accredited 275-MED Version date: 03/08/2021 Accredited:
UZ Brussel - Molecular Pathology	solid tumors - gene panels	Freya Vaeyens	NGS targeted sequencing after hybridization capture, home brew STHT assay (solid and hematological tumors) Genes list and indications available at http://www.brightcore.be/stht Sample Type: FFPE Tissue Frequency: 1x/week Accreditation: BELAC 141-MED Version date: 10/10/2019 Accredited:
AZ St-Jan Brugge - Pathologie	solid tumors - gene panels	Dr. Ivo Van Den Berghe - Dr. Jacques Van Huysse - Ing. Stefanie Vermeire	SAMPLE TYPE: FFPE tissues FREQUENCY: 1X/week TEST TYPE: NGS Genes under accreditation: EGFR, KRAS, NRAS, BRAF Other genes: IDH1, IDH2, KIT, PDGFRA Version date: 29/11/2017 Accredited:
UZ Gent	solid tumors - gene panels	Joni Van der Meulen Siebe Loontiens Suzanne Vanhauwaert	Sample Type: FFPE Tissue Frequency: 1x/week SARCOMA MDG RNA NGS panel for detection of fusions and splice variants (55 genes, Archer FusionPlex): ALK, BCOR, BRAF, CAMTA1, CIC, CSF1, CTNNB1, EGFR, EPC1, ERG, ESR1, EWSR1, FGFR1, FGFR2, FGFR3, FOS, FOSB, FOXO1, FUS, GLI1, HMGA2, JAZF1, MDM2, MEAF6, MET, MGEA5, MKL2, MYOD1, NCOA1, NCOA2, NR4A3, NTRK1, NTRK2, NTRK3, NUTM1, PAX3, PDGFB, PHF1, PLAG1, PRKCA, PRKCB, PRKCD, RAF1, RET, ROS1, SS18, STAT6, TAF15, TCF12, TFE3, TFG, USP6, VGLL2, YAP1, YWHAE BELAC accreditation number (351-MED) Version date: 16/03/2021 Accredited:
UZ Brussel - Molecular Pathology	solid tumors - gene panels	Freya Vaeyens	Fusion NGS panel - TSO500 Illumina Sample Type: FFPE tissue Frequency: 1x/week Version date: 10/02/2021 Accredited:
Jessa Hospital	solid tumors - gene panels	Dr. Brigitte Maes, Guy Froyen	Comprehensive genomic tumor profiling (CGP) with TSO500 assay (Illumina) Detection of SNVs and indels in 523 cancer-related genes, CNVs in 57 genes, gene fusions in 55 driver genes and exon skipping events in 3 relevant genes. Moreover analysis of Tumor Mutation Burden (TMB) and MicroSatellite Instability (MSI) of the tumor specimen is performed. 2x/week NextSeq500/550; 8 DNA + 8 RNA samples on a High Output flowcell Accredited 275-MED Version date: 03/08/2021 Accredited:
IPG Gosselies	solid tumors - gene panels	P. Vannuffel C. De Rop	In-house NGS gene panel for the detection of mutations and indels in the following genes: Lung panel (AKT1, ALK, BRAF, DDR2, EGFR, ERBB2, KRAS, MEK1, NRAS, PIK3CA, PTEN (+MET exon 14 skipping by RT-PCR) Colon panel (BRAF, KRAS, NRAS, PIK3CA) Melanoma panel (BRAF, KIT, NRAS, TERT promoter) GIST panel (KIT, PDGFRA) Brain panel (BRAF, H3F3A, IDH1/2, TERT promoter) Breast panel (ESR1, PIK3CA) Thyroid panel (BRAF, KRAS, NRAS, HRAS, TP53, TERT promoter) Pancreas panel (GNAS, KRAS) NGS AmpliSeq - Ion Torrent Sample type: FFPE BELAC accreditation number: 381-MED Version date: 02/09/2022 Accredited:
IPG Gosselies	solid tumors - gene panels	P. Vannuffel C. De Rop	Cancer hotspot panel v2 (AmpliSeq) NGS AmpliSeq - Ion Torrent Sample type: FFPE BELAC accreditation number: 381-MED. Version date: 02/09/2022 Accredited:
IPG Gosselies	solid tumors - gene panels	P. Vannuffel C. De Rop	In-house NGS gene panel for the detection of mutations and indels in the following genes for Lung Cancer: AKT1, ALK, BRAF, DDR2,EGFR,ERBB2,KRAS,MEK1,NRAS,PIK3CA,PTEN Analysis is not accreditated for the following genes/cancers: Colon panel (BRAF,KRAS,NRAS,PIK3CA) Breast panel (ESR1,PIK3CA) NGS AmpliSeq - Ion Torrent Sample type: Plasma BELAC accreditation number: 381-MED Version date: 07/09/2022 Accredited:
IPG Gosselies	solid tumors - gene panels	P. Vannuffel C. De Rop	Archer FusionPlex Lung v1 for the detection of fusions and splice variants in the following genes: ALK, EGFR, FGFR1, FGFR2, FGFR3, MET, NRG1, NTRK1, NTRK2, NTRK3, RET and ROS. NGS Archer - Illumina. Sample type: FFPE. BELAC accreditation number: 381-MED. Version date: 07/09/2022 Accredited:
IPG Gosselies	solid tumors - gene panels	P. Vannuffel C. De Rop	Archer FusionPlex Expanded Sarcome for the detection of fusions and splice variant in the following genes: ALK, BCOR, BRAF, CAMTA1, CIC, CSF1, EGFR, EPC1, ERG, ESR1, EWSR1, FGFR1, FGFR2, FGFR3, FOS, FOSB, FOXO1, FUS, GLI1, HMGA2, JAZF1, MDM2, MEAF6, MET, MGEA5, MKL2, MYOD1, NCOA1, NCOA2, NR4A3, NTRK1, NTRK2, NTRK3, NUTM1, PAX3, PDGFRB, PHF1, PLAG1, PRKCA, PRKCB, RAF1, RET, ROS1, SS18, STAT6, TAF15, TCF12, TFE3, TFG, USP6, VGLL2, YAP1 and YWHAE. NGS Archer - Illumina Sample type: FFPE Version date: 07/09/2022 Accredited:

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