Cytomegalovirus (CMV) DNA

Qualitative and/or quantitative detection of cytomegalovirus (CMV) DNA
Lab Test name Contact Comments
AZ St-Jan Brugge - Laboratoriumgeneeskunde Cytomegalovirus (CMV) DNA Dr. M. Reynders / Patrick Descheemaeker
SAMPLE TYPE:
(min 550µl) EDTA plasma, serum, Urine, BAL, CSF, amnionvocht, klierweefsel, EDTA-volbloed neonaten, oogvochten en biopten
FREQUENCY:
2x/week (Tuesday,Friday)
TEST TYPE:
In house real-time PCR, quantitative
Version date: 17/11/2017
Accredited:
UZ Brussel - Microbiology Cytomegalovirus (CMV) DNA Dr. Denis Piérard, Oriane Soetens
SAMPLE TYPE:
-plasma (min. 0.6 ml)
-amniotic fluid (min. 0.6 ml)
-CSF (min. 0.1 ml)
-dried blood spot (min. 2)
FREQUENCY: 1x/week (wednesday)
TEST TYPE: in-house real-time PCR, qualitative
Cobas Ampliprep/ Cobas TaqMAN CMV test(Roche Diagnostics), quantitative
Version date: 26/09/2017
Accredited:
UZA Mol. Diagn. Cytomegalovirus (CMV) DNA D. Ursi, K. Bergs, M. Michiels, V. Matheeussen
Sample type:
- EDTA plasma (at least 550 µl)
- cerebrospinal fluid (at least 250 µl)
- ocular fluid (at least 250 µl)
- amniotic fluid (at least 1,1 ml)
- biopsies (not fixed!)

Frequency: 3 times a week

Test type: in house real-time PCR
Jessa Hospital Cytomegalovirus (CMV) DNA dr. Cartuyvels, Raymaekers Marijke
SAMPLE TYPE:
-EDTA whole blood
-bronchoalveolar lavage
-CSF
-amniotic fluid
FREQUENCY: 2x/week
TEST TYPE: in house real-time quantitative PCR
Accredited:
OLVZ Aalst Cytomegalovirus (CMV) DNA Anne Vankeerberghen
Sample type:
EDTA-blood (500 µl)
CSF(500 µl)
Respiratory sample (500 µl)
Urine midstream (500 µl)

Frequency: Daily (except weekend)

Test type: In house real time PCR
Version date: 29/09/2017
Accredited:
CHU UCL NAMUR Cytomegalovirus (CMV) DNA pierre.bogaerts@uclouvain.be
Sample type:
- EDTA blood (at least 250 µl).
Test performed on Tuesday and Friday
Quantitative and qualitative in house real-time PCR. Counting in copies/mL or copies/200.000 WBC.
UZ Leuven MolPLUZ Cytomegalovirus (CMV) DNA Marc Van Ranst, Katrien Lagrou
Sample type:
- EDTA-blood
- Guthrie/ blood spot
- CSV
- ocular fluid
- amniotic fluid
- biopsy

Minimal sample volume:1ml EDTA blood - 250 µl all other samples
Frequency: 2-5 times a week
Test type: Quantitative in-house realtime PCR (500-5E+6 copies/ml)
Remark: also included in respiratory-panel-assay on swab, aspirate and BAL
CHU Saint-Pierre Cytomegalovirus (CMV) DNA Reynders Marijke, Maaroufi Younes, Scheen Robert
Staaltypes: EDTA-bloedstalen, CSV, beenmerg, biopten, amnionvocht, placenta, oogvochten, BAL-vochten

Minimum staalvolume: 250 microliter

Kwantitatieve in house real-time PCR
AZ Delta Cytomegalovirus (CMV) DNA Steven Vervaeke/Joke Breyne
SAMPLE TYPE:
- EDTA plasma (min 0.5ml)
- serum, Urine, BAL, CSF
FREQUENCY: 1x/week (Wednesday)
TEST TYPE: Diagenode Real-Time CMV test
Version date: 05/12/2017
Accredited:
ZNA Middelheim Cytomegalovirus (CMV) DNA Kim Camps
Sample type:EDTA plasma
Frequency: twice a week
Test type: in hous quantitative RT-PCR
Labo Cliniques Sud Luxembourg Cytomegalovirus (CMV) DNA Nicolas Hougardy
SAMPLE TYPE:
- Plasma (EDTA) (min 1ml)
- Urine (min 1ml)
- BAL (min 1ml)
- CSF (min 500µL)
FREQUENCY: 1x/week
TEST TYPE: "CMV Kit" (Diagenode), COBAS TaqMan 48 (Roche)
UZ Gent Cytomegalovirus (CMV) DNA Elizaveta Padalko
Sample Type:
-EDTA blood (min. 0.5ml)
-amniotic fluid (min. 0.2ml)
-bronchoalveolar lavage
-CSF (min. 0.2ml)
-dried blood spot
-biopsies (not fixed!)
Frequency: 2x/week (Monday-Thursday)
Test Type: semi-kwantitative in-house real-time PCR
LMMI Tilburg (NL) Cytomegalovirus (CMV) DNA Dr John WA Rossen
Organisme: CMV
Bepaling: DNA amplificatie
Methode: REal-time PCR (Taqman)
Soort bepaling: Kwalitatief
Typering: Nee
Frequentie Dagelijks maandag t/m vrijdag
commercieel: nee
Externe QC: QCMD
Materiaal: EDTA-bloed, bronchiaal secreet, Bronchiolaire lavage (BAL), urine, sputum
Ziekenhuis Oost-Limburg Cytomegalovirus (CMV) DNA Dr. Els Oris, Katrien Vandebroek
Sample type:
urine
blood
respiratory samples

Frequency:
2x/week

Test type:
in-house real-time PCR
Imelda-Bonheiden Cytomegalovirus (CMV) DNA Dr. Frans, Isabel Micalessi
Sample type:
- BAL
- Biopt
- Urine
- Amniosfluid
- eSwab (mouth)
Frequency: Daily (Amniosfluid), 2x/week (BAL/Urine/eSwab)
Test type: CMV Real-time PCR (Rotorgene,Diagenode)

Sample type:
- CSV
Frequency: Daily
Test type: Meningitis-Encephalitis Panel (FilmArray, BioMerieux)
GZA St-Augustinus Cytomegalovirus (CMV) DNA Philippe Van Lint
performed on urine

in-house, qualitative real-time PCR

frequency: daily (Monday-Friday)
Version date: 12/07/2017
Accredited: