Herpes simplex virus (HSV) DNA

Qualitative and/or quantitative detection of Herpes Simplex Viruses DNA
Lab Test name Contact Comments
UZ Leuven MolPLUZ Herpes simplex virus (HSV) DNA Marc Van Ranst, Katrien Lagrou
Sample type:
- CSV
- ocular fluid
- biopsy

Minimal sample volume: 250 µl
Frequency: 2-5 times a week
Test type: In-house realtime PCR with genotyping HSV-1 / HSV-2
Remark: also included in respiratory-panel-assay on swab, aspirate and BAL
UZA Mol. Diagn. Herpes simplex virus (HSV) DNA D. Ursi, K. Bergs, M. Michiels, V. Matheeussen
Sample type:
- cerebrospinal fluid
- ocular fluids or swabs
- BAL
(at least 250 µl).

Frequency: 3 times a week

Test type: in house real-time PCR
UZ Brussel - Microbiology Herpes simplex virus (HSV) DNA Dr. Denis Piérard, Oriane Soetens
SAMPLE TYPE:
-CSF (min 0.2 ml)
FREQUENCY: 2 to 3x/week
TEST TYPE: commercial real-time PCR (HSV1 HSV2 VZV r-gene kit, Argene)
Version date: 26/09/2017
Accredited:
Jessa Hospital Herpes simplex virus (HSV) DNA dr. Cartuyvels, Raymaekers Marijke
SAMPLE TYPE:
-CSF
-Bronchoalveolar lavage
-ocular fluids or swabs
FREQUENCY: from Monday till Friday
TEST TYPE: in house real-time qualitative PCR
Accredited:
OLVZ Aalst Herpes simplex virus (HSV) DNA Anne Vankeerberghen
Sample type:
Skin lesion swab
Eye swab
Urogenital swab
CSF (500 µl)
Respiratory sample (500 µl)

Frequency: Daily (except weekend)
Test type: In house real time PCR
Version date: 29/09/2017
Accredited:
Ziekenhuis Oost-Limburg Herpes simplex virus (HSV) DNA Dr. Els Oris, Katrien Vandebroek
Sample type:
CSF
respiratory specimens
swabs

Frequency:
CSF: daily
2x/week (swabs and respiratory specimens)

Test type:
QIAsymphony extraction
in house real time PCR
CHU UCL NAMUR Herpes simplex virus (HSV) DNA pierre.bogaerts@uclouvain.be
Sample type:
-Skin lesion swab, ...
-CSF (200 µl)
-Biopsy
Test performed on Tuesday and Friday
In house Real-time PCR. Global detection of HSV1 and 2.
AZ St-Lucas Gent Herpes simplex virus (HSV) DNA Dr A.M. van den Abeele/Elke Vanlaere
Sample type: CSF, BAL, Skin lesion swab, Eye swab
Frequency: daily (except weekend)
Test type: HSV1/HSV2/VZV R-gene kit (Argene), Quantitative PCR

Or: Urogenital swab (Eswab)
Frequency: 1x/week
Test type: in house Realtime quantitative PCR
Imelda-Bonheiden Herpes simplex virus (HSV) DNA Dr. Johan Frans, Isabel Micalessi
Sample type:
-BAL
-Respiratory specimens
-Swabs
Frequency: Daily (Ocular fluid), 2x/week (BAL/eSwabs)
Test type: Real-time PCR (Artus HSV-1/2 RG PCR Kit (Qiagen))

Sample type:
- CSV
Frequency: Daily
Test type: Meningitis-Encephalitis Panel (FilmArray, BioMerieux)
GZA St-Augustinus Herpes simplex virus (HSV) DNA Philippe Van Lint
performed on CSF (min 350µl) and swabs (eSwab, COPAN)

in-house, qualitative real-time PCR

discriminating HSV-1/HSV-2

frequency: daily (Monday-Friday)
Accredited:
CHU Saint-Pierre Herpes simplex virus (HSV) DNA Reynders Marijke, Maaroufi Younes, Scheen Robert
Staaltypes: CSV, biopten, oogvocht, EDTA-bloed (enkel voor vermoeden herpes neonatorum)

Minimum staalvolume: 250 microliter

Kwalitatieve in house real-time PCR
ZNA Middelheim Herpes simplex virus (HSV) DNA Walter Verstrepen
Sample type:
- cerebrospinal fluid
- EDTA whole blood
- ocular fluids or swabs
- BAL
(at least 250 µl).

Frequency: every working day
Test type: in house real-time PCR
Labo Cliniques Sud Luxembourg Herpes simplex virus (HSV) DNA Nicolas Hougardy
SAMPLE TYPE:
- CSF (min 200µl)
- BAL (min 1ml)
- Swabs
FREQUENCY: 1x/week
TEST TYPE: in-house, real time PCR (Glycoprotein D gene detection), LightCycler 2.0 (Roche)
ARTICLE: "UZ-Leuven protocol(CEMOL)"
LMMI Tilburg (NL) Herpes simplex virus (HSV) DNA Dr John WA Rossen
Organisme: HSV-1 / HSV-2
Bepaling: DNA amplificatie
Methode: Real-time PCR (Taqman)
Soort bepaling: Kwalitatief
Typering: HSV1 en HSV2
Frequentie: Dagelijks maandag t/m zaterdag
Status: Routine
Commercieel: Nee
Externe QC: QCMD
Materiaal: Bronchiolaire lavage (BAL), neus / keelwat, liquor, genitale swab
pus, weefselbiopt, blaasjesvocht
AZ St-Jan Brugge - Laboratoriumgeneeskunde Herpes simplex virus (HSV) DNA Dr. M. Reynders / P. Descheemaeker
Sample:
(min 550 µl)
CSV, BAL, EDTA-plasma (neonates), swab in UTM, biopten uit respiratoire of gastro-intestinale tractus, genital monsters, amnionfluid, voorkamer- en vitreumvocht 
Frequency:
2x/week (Tuesday, Friday)
Methode:
in house real time PCR
Version date: 17/11/2017
Accredited:
Imelda-Bonheiden Herpes simplex virus (HSV) DNA Dr. Johan Frans, Isabel Micalessi
Sample type: CSF
Frequency: Daily
Test type: Meningitis-Encephalitis Panel (FilmArray, BioMerieux)