Sample type:
- CSV
- ocular fluid
- biopsy

Minimal sample volume: 250 µl
Frequency: every weekday (csv : every day 7/7)
Test type: In-house realtime PCR with genotyping HSV-1 / HSV-2
Remark: also included in respiratory-panel-assay on swab, aspirate and BAL
Accreditation BELAC: 215-MED
Accredited:

Sample type:
- cerebrospinal fluid
- ocular fluids or swabs
- BAL
(at least 250 µl).

Frequency: 3 times a week

Test type: in house real-time PCR

SAMPLE TYPE:
-CSF (min 0.2 ml)
FREQUENCY: 2 to 3x/week
TEST TYPE: commercial real-time PCR (HSV1 HSV2 VZV r-gene kit, Argene)
Version date: 26/09/2017
Accredited:

SAMPLE TYPE:
-CSF
-Bronchoalveolar lavage
-ocular fluids or swabs
FREQUENCY: from Monday till Friday
TEST TYPE: in house real-time qualitative PCR
Accredited:

Sample type:
Skin lesion swab
Eye swab
Urogenital swab
CSF (500 µl)
Respiratory sample (500 µl)

Frequency: Daily (except weekend)
Test type: In house real time PCR
Version date: 06/05/2019
Accredited:

Sample type:
CSF
respiratory specimens
swabs

Frequency:
CSF: daily
2x/week (swabs and respiratory specimens)

Test type:
QIAsymphony extraction
in house real time PCR

Sample type:
-Skin lesion swab, ...
-CSF (200 µl)
-Biopsy
Test performed on Tuesday and Friday
In house Real-time PCR. Global detection of HSV1 and 2.

Sample type: CSF, BAL, Skin lesion swab, Eye swab
Frequency: daily (except weekend)
Test type: HSV1/HSV2/VZV R-gene kit (Argene), Quantitative PCR

Or: Urogenital swab (Eswab)
Frequency: 1x/week
Test type: in house Realtime quantitative PCR

Sample type:
-BAL
-Respiratory specimens
-Swabs
Frequency: Daily (Ocular fluid), 2x/week (BAL/eSwabs)
Test type: Real-time PCR (Artus HSV-1/2 RG PCR Kit (Qiagen))

Sample type:
- CSV
Frequency: Daily
Test type: Meningitis-Encephalitis Panel (FilmArray, BioMerieux)

This PCR test is performed on swabs.
Frequency: daily (Monday-Saturday)

in-house, qualitative real-time PCR
discriminating HSV-1/HSV-2
--------------------------------------------

This PCR test is performed on CSV.
Frequency: daily (Monday-Saturday)

The CSV PCR is part of the following syndromic panel: QIAstat-Dx Meningitis/Encephalitis (ME) Panel

Bacteria
Streptococcus pneumoniae
Neisseria meningitidis
Streptococcus agalactiae
Listeria monocytogenes
Haemophilus influenzae
Escherichia coli K1
Streptococcus pyogenes
Mycoplasma pneumoniae

Viruses
Enterovirus
Herpes simplex virus 1
Herpes simplex virus 2
Humaan parechovirus
Humaan herpes virus 6
Varicella zoster virus

Fungi
Cryptococcus gattii/neoformans

BELAC accreditation number 404-MED
Accredited:

Staaltypes: CSV, biopten, oogvocht, EDTA-bloed (enkel voor vermoeden herpes neonatorum)

Minimum staalvolume: 250 microliter

Kwalitatieve in house real-time PCR

Sample type:
- cerebrospinal fluid
- EDTA whole blood
- ocular fluids or swabs
- BAL
(at least 250 µl).

Frequency: every working day
Test type: in house real-time PCR

SAMPLE TYPE:
- CSF (min 200µl)
- BAL (min 1ml)
- Swabs
FREQUENCY: 1x/week
TEST TYPE: in-house, real time PCR (Glycoprotein D gene detection), LightCycler 2.0 (Roche)
ARTICLE: "UZ-Leuven protocol(CEMOL)"

Organisme: HSV-1 / HSV-2
Bepaling: DNA amplificatie
Methode: Real-time PCR (Taqman)
Soort bepaling: Kwalitatief
Typering: HSV1 en HSV2
Frequentie: Dagelijks maandag t/m zaterdag
Status: Routine
Commercieel: Nee
Externe QC: QCMD
Materiaal: Bronchiolaire lavage (BAL), neus / keelwat, liquor, genitale swab
pus, weefselbiopt, blaasjesvocht

Sample:
(min 550 µl)
CSV, BAL, EDTA-plasma (neonates), swab in UTM, biopten uit respiratoire of gastro-intestinale tractus, genital monsters, amnionfluid, voorkamer- en vitreumvocht 
Frequency:
2x/week (Tuesday, Friday)
Methode:
in house real time PCR
Version date: 17/11/2017
Accredited:

Sample Type: eSwab (urogenital or wound/pus)
Frequency: Daily
Test Type: RT-PCR; ARIES HSV1&2 Assay (Aries, Diasorin)

Sample Type: Cerebrospinal fluid
Frequency: Daily
Test Type: RT-PCR; QIAStat-Dx Meningitis/Encephalitis panel (QIAStat, Qiagen)

The ME test also screens for the following targets:
Viruses: Enterovirus, Human parechovirus, Human herpes virus 6, Varicella zoster virus
Bacteria: Streptococcus pneumoniae, Neisseria meningitidis, Streptococcus agalactiae, Listeria monocytogenes, Haemophilus influenza, Escherichia coli K1, Streptococcus pyogenes, Mycoplasma pneumoniae
Fungi: Cryptococcus neoformans/gattii

Belac accreditation number 523-MED
Version date: 07/07/2022
Accredited:

Sample type: CSF
Frequency: Daily
Test type: Meningitis-Encephalitis Panel (FilmArray, BioMerieux)

HSV 1 / HSV 2
Sample type: Urogenital swab, urine, Eswab
Frequency: 1-3/week
Test type: Genital Ulcer panel Seegene (Starlet)
Version date: 26/10/2022

Sample type: CSF
Frequency: daily
Test type: Filmarray ME panel (Biofire, also during weekend), confirmation with HSV1&2 VZV R-gene kit (Biomérieux) and EMAG extraction

Sample type: UTM, eSwab
Frequency: daily (mon-fri)
Test type: HSV1&2 VZV R-Gene (Biomérieux) with EMAG extraction

Accreditation: 376-MED
Version date: 13/09/2021
Accredited: