Influenza A and B

Qualitative and/or quantitative detection of influenza viruses RNA
Lab Test name Contact Comments
OLVZ Aalst Influenza A and B Anne vankeerberghen
Sample type:
Nasopharyngeal swab
Respiratory sample (500 µl)

Frequency: Daily (except weekend)
Test type: In house real time PCR
Version date: 29/09/2017
Accredited:
UZ Leuven MolPLUZ Influenza A and B Marc Van Ranst, Katrien Lagrou
Sample type:
- respiratory swab
- respiratory samples

Minimal sample volume: 1ml
Frequency: every day (monday to friday)
Test type: In-house realtime PCR with genotyping A/B
Remark: also included in respiratory-panel-assay on swab, aspirate and BAL
CHU Saint-Pierre Influenza A and B Reynders Marijke, Maaroufi Younes, Scheen Robert
Staaltypes: Respiratoire stalen (nasopharyngeale wissers, keelwissers, nasopharynxaspiratie, BAL, tracheobroncheolaire aspiratie)

Minimum staalvolume: 500 microliter

Kwalitatieve in house real-time PCR
ZNA Middelheim Influenza A and B Walter Verstrepen
Sample type:
Respiratory specimens (at least 250 µl)

Frequency: every working day
Test type: In house real time PCR
AZ St-Jan Brugge - Laboratoriumgeneeskunde Influenza A and B Dr. M. Reynders / Patrick Descheemaeker
SAMPLE TYPE:
respiratory sample (min 550 µl)
FREQUENCY:
daily
TEST TYPE: TaqMan Array Card, in house real time PCR
further subtyping for FluA (H1N1, H3N2, H7N9)
Version date: 17/11/2017
AZ Damiaan Oostende Influenza A and B Ben Vanmassenhove/ Anne-Sophie Hervent
SAMPLE TYPE: flocked swabs
FREQUENCY: 1-3/week
TEST TYPE: Real Time PCR (Erasmus Protocol)
Accredited:
Imelda-Bonheiden Influenza A and B Dr. Johan Frans, Isabel Micalessi
Sample Type:
- NAP in UTM
- BAL
Frequency: daily
Test Type: NxTAG Respiratoir Pathogeen Panel (MAGPIX, Luminex)
AZ St-Lucas Gent Influenza A and B Dr A.M. van den Abeele/Elke Vanlaere
SAMPLE TYPE:
-preferential: nasopharyngeal aspirate (min. 0.2 ml)
-alternative: BAL
FREQUENCY: 2x/week
TEST TYPE: Smartfinder (Pathofinder)
LMMI Tilburg (NL) Influenza A and B Dr John WA Rossen
Organisme: Influenza A / Influenza B
Bepaling: cDNA amplificatie
Methode: Real-time RT-PCR (Taqman)
Soort bepaling: Kwalitatief
Typering: H-typering (H1, H3, H5, MexFlu)
Frequentie: Dagelijks maandag t/m zaterdag
Status: Routine
Commercieel: Nee
Externe QC: QCMD
Materiaal neus / keelwat, sputum, bronchiaal secreet, Bronchiolaire lavage (BAL)
Ziekenhuis Oost-Limburg Influenza A and B Dr. Els Oris, Katrien Vandebroek
Sample type:
swabs

Frequency:
daily

Test type:
Xpert Flu/RSV (cepheid)
Jessa Hospital Influenza A and B dr Cartuyvels, Raymaekers Marijke
SAMPLE TYPE:
-nasopharyngeal aspirate or secretion
-nasopharyngeal e-swab (UTM or liquid amies)
-BA
FREQUENCY: Monday till Friday
TEST TYPE: in-house real time PCR
Accredited:
UZ Brussel - Microbiology Influenza A and B Denis Piérard, Oriane Soetens
SAMPLE TYPE: Nasopharyngeal swab or aspirate, BAL (min. 0.6 ml)
FREQUENCY: every weekday in the respiratory season
TEST TYPE: commercial real-time PCR (Xpert Flu/RSV XC kit, Cepheid)
Version date: 26/09/2017
GZA St-Augustinus Influenza A and B Philippe Van Lint
This PCR test is performed on respiratory samples (500µL).
Frequency: daily (Monday-Friday)

This respiratory panel screens for the following targets:
Viruses: Adenovirus, Bocavirus, Coronavirus, Enterovirus, Human metapneumovirus, (Para-)Influenza virus, Rhinovirus, RSV.
Bacteria: Bordetella (para-)pertussis, Bordetella holmesii, Chlamydophila pneumoniae, Legionella pneumophila, Mycoplasma pneumoniae.
Version date: 30/01/2018