Detection of mutations in the ABL kinase (ATP binding domain and P-loop). Resistance mechanism to Gleevec, Dasatinib, etc.
Lab
Test name
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IPG Gosselies
Abelson (ABL1) mutation analysis
pascal vannuffel
AZ St-Jan Brugge - Laboratoriumgeneeskunde
Abelson (ABL1) mutation analysis
dr. Helena Devos/Friedel Nollet, Ph.D.
Long-range PCR + direct Sanger sequencing of ABL1 kinase domain
Version date: 04/11/2017 Accredited:
CUB Erasme - Medical Genetics Dpt
Abelson (ABL1) mutation analysis
Pierre Heimann/Barbara Dessars/Hakim El Housni
based on Branford S et al. protocol
PCR on cDNA followed by sequence analysis
UZ Leuven MolPLUZ
Abelson (ABL1) mutation analysis
Lucienne Michaux / Els Lierman
Sample type: blood / bone marrow (EDTA, 2-5 ml)
Frequency: 2x/week
Test type: digital droplet PCR (ddPCR) on DNA with PrimePCR ddPCR Mutation Assay (Bio-Rad) for BCR-ABL1 p.Q252H, p.Y253H, p.E255K, p.T315I, p.F317L, p.F359V
Accredited: