CEBPalpha mutation analysis

Lab	Test name	Contact	Comments
UZ Brussel - Molecular Hematology	CEBPalpha mutation analysis	Marleen Bakkus/Christian Demanet	At diagnosis or relapse: DNA test, PCR (TAD1, TAD2 and bZIP domain) and capillary electrphoresis. Multiplex assay with Flt3-ITD and NPM1 mutation analysis. Consensus primers from working group MolecularDiagnostics.be. Version date: 26/09/2017 Accredited:
AZ St-Jan Brugge - Laboratoriumgeneeskunde	CEBPalpha mutation analysis	dr. Helena Devos/Friedel Nollet, Ph.D.	At diagnosis or relapse: DNA test, PCR (TAD1, TAD2 and bZIP domain) and capillary electrphoresis. Multiplex assay with Flt3-ITD and NPM1 mutation analysis. Consensus primers from working group MolecularDiagnostics.be. DNA sequencing of all three amplicons is performed when at least one insertion/deletion mutation is observed. Version date: 04/11/2017
UZ Gent	CEBPalpha mutation analysis	Barbara Denys, Karl Vandepoele	Simultaneous detection with FLT3-ITD and NPM1 DNA test CEBPA is also present in our NGS panel for myeloid malignancies Version date: 18/08/2017 Accredited:
UZ Leuven MolPLUZ	CEBPalpha mutation analysis	Lucienne Michaux / Els Lierman	Sample type: blood / bone marrow (EDTA, 2-5 ml) Frequency: 1x/week Test type: PCR on DNA, adapted from Benthaus T. et al, Br J Haematol 2008. Can also be performed on RNA. Accredited:
CUB Erasme - Medical Genetics Dpt	CEBPalpha mutation analysis	Pierre Heimann/Barbara Dessars/Hakim El Housni	PCR and capillary electrophoresis Analytical sensitivity : 5%
AZ Delta	CEBPalpha mutation analysis	Geert Martens, Elke Boone	SAMPLE TYPE: blood or bone marrow (1 ml) FREQUENCY: upon request, at diagnosis or relapse TEST TYPE: DNA test, PCR (TAD1, TAD2 and bZIP domain) and capillary electrophoresis. Multiplex assay with Flt3-ITD and NPM1 mutation analysis. Consensus primers from working group MolecularDiagnostics.be. Version date: 05/12/2017 Accredited: