CEBPalpha mutation analysis

Lab	Test name	Contact	Comments
CHU Liège - Hemato	CEBPalpha mutation analysis	Dr Frédéric Lambert	EDTA anticoagulated bone marrow (3 ml). DNA PCR followed with Fragment analysis, direct sequencing confirmation for suspected mutant. As described by Lin et al. Leukemia, 2006.
UZ Brussel - Molecular Hematology	CEBPalpha mutation analysis	Marleen Bakkus/Christian Demanet	At diagnosis or relapse: DNA test, PCR (TAD1, TAD2 and bZIP domain) and capillary electrphoresis. Multiplex assay with Flt3-ITD and NPM1 mutation analysis. Consensus primers from working group MolecularDiagnostics.be. Version date: 26/09/2017 Accredited:
AZ St-Jan Brugge - Laboratoriumgeneeskunde	CEBPalpha mutation analysis	dr. Helena Devos/Friedel Nollet, Ph.D.	At diagnosis or relapse: DNA test, PCR (TAD1, TAD2 and bZIP domain) and capillary electrphoresis. Multiplex assay with Flt3-ITD and NPM1 mutation analysis. Consensus primers from working group MolecularDiagnostics.be. DNA sequencing of all three amplicons is performed when at least one insertion/deletion mutation is observed. Version date: 04/11/2017
UZ Gent	CEBPalpha mutation analysis	Barbara Denys, Karl Vandepoele	Simultaneous detection with FLT3-ITD and NPM1 DNA test CEBPA is also present in our NGS panel for myeloid malignancies Version date: 18/08/2017 Accredited:
UZ Leuven MolPLUZ	CEBPalpha mutation analysis	Lucienne Michaux / Els Lierman	Sample type: blood / bone marrow (EDTA, 2-5 ml) Frequency: 1x/week Test type: PCR on DNA, adapted from Benthaus T. et al, Br J Haematol 2008. Can also be performed on RNA. Accredited:
CUB Erasme - Medical Genetics Dpt	CEBPalpha mutation analysis	Pierre Heimann/Barbara Dessars/Hakim El Housni	PCR and capillary electrophoresis Analytical sensitivity : 5%
CHU Liège - Hemato	CEBPalpha mutation analysis	Dr Frédéric LAMBERT	Familial MDS/AML; Inherited predisposition syndrome to MDS/AML. Screening could be performed on bone marrow BUT confirmation of germinal status request skin biopsy/fibroblasts grouwth. Informed consent formulaire requested: http://www.chu.ulg.ac.be/jcms/c_9635594/informations-pratiques-relatives-aux-analyses
AZ Delta	CEBPalpha mutation analysis	Geert Martens, Elke Boone	SAMPLE TYPE: blood or bone marrow (1 ml) FREQUENCY: upon request, at diagnosis or relapse TEST TYPE: DNA test, PCR (TAD1, TAD2 and bZIP domain) and capillary electrophoresis. Multiplex assay with Flt3-ITD and NPM1 mutation analysis. Consensus primers from working group MolecularDiagnostics.be. Version date: 05/12/2017 Accredited: