WT1 overexpression analysis

Lab	Test name	Contact	Comments
UZA Mol. Diagn.	WT1 overexpression analysis	K.Vermeulen, M-B Maes	Real-time kwantitatieve PCR met de WT1 mRNA OneStep assay (Otsuka Pharmaceutical) Lightcycler 480 Version date: 05/12/2017 Accredited:
UZ Gent	WT1 overexpression analysis	Barbara Denys, Karl Vandepoele	Real-time PCR according to ELN guidelines (Cilloni et al., J Clin Oncol 2009) RNA-test Version date: 11/10/2017 Accredited:
CHU Liège - Hemato	WT1 overexpression analysis	Dr Frédéric Lambert	QIAGEN, ipsogen WT1 ProfileQuant Kit CE
CUB Erasme - Medical Genetics Dpt	WT1 overexpression analysis	Pierre Heimann/Barbara Dessars/Hakim El Housni	Home-made assay. Test performed exclusively on peripheral blood
IPG Gosselies	WT1 overexpression analysis
AZ Delta	WT1 overexpression analysis	Elisabeth Moreau/Elke Boone	SAMPLE TYPE: Blood or bone marrow (1ml, EDTA) FREQUENCY:1x/2-weeks TEST TYPE: real-time PCR in house protocol (Taqman gene expression assay for WT1 (exon 6/7) and ABL). Version date: 05/12/2017 Accredited:
ZNA Middelheim	WT1 overexpression analysis	Pieter De Schouwer	In house method, normalised copy numbers / ABL. Both Peripheral blood and bone marrow. Lab specific reference values.
UZ Leuven MolPLUZ	WT1 overexpression analysis	Lucienne Michaux / Els Lierman	Sample type: blood / bone marrow (EDTA, 2-5 ml) Frequency: 2x/week Test type: real time PCR on cDNA (WT1 ProfileQuant Kit, Ipsogen)