WT1 overexpression analysis

Lab	Test name	Contact	Comments
UZA Mol. Diagn.	WT1 overexpression analysis	K. Vermeulen, M. Le Mercier, M-B Maes	Quantitative RT-PCR with the WT1 mRNA OneStep assay (Otsuka Pharmaceutical) on Lightcycler 480 Sample Type: Blood, Bone marrow BELAC accreditation number 204-MED Version date: 06/09/2022 Accredited:
UZ Gent	WT1 overexpression analysis	Barbara Denys, Karl Vandepoele	Real-time PCR according to ELN guidelines (Cilloni et al., J Clin Oncol 2009) RNA-test BELAC accreditation number 087-MED Version date: 16/03/2021 Accredited:
LHUB-ULB	WT1 overexpression analysis	Pierre Heimann/Laurent Dewispelaere/Pascale Cochaux/Hakim El Housni	SAMPLE TYPE: Peripheral Blood exclusively (EDTA, 5 mL) TRANSPORT: room temperature, within 24h after sampling FREQUENCY: upon request; max TAT: 4 weeks TEST TYPE: One Step RT droplet digital PCR (Dewispelaere et al. JMD 2020). Laboratory developed test (LDT) BELAC accreditation number 650-MED Version date: 04/10/2021 Accredited:
IPG Gosselies	WT1 overexpression analysis	P. Vannuffel C. De Rop	In house method base on Cilloni et al, Leukemia, 2002. Quantitative RT-PCR. Sample type: Blood and bone marrow. BELAC accreditation number: 381-MED Version date: 02/09/2022 Accredited:
AZ Delta	WT1 overexpression analysis	Elisabeth Moreau/Elke Boone	SAMPLE TYPE: Blood or bone marrow (1ml, EDTA) FREQUENCY:1x/2-weeks TEST TYPE: real-time PCR in house protocol (Taqman gene expression assay for WT1 (exon 6/7) and ABL). Version date: 05/12/2017 Accredited:
ZNA Middelheim	WT1 overexpression analysis	Pieter De Schouwer	In house method, normalised copy numbers / ABL. Both Peripheral blood and bone marrow. Lab specific reference values.
UZ Leuven MolPLUZ	WT1 overexpression analysis	Jolien De Bie	Sample type: blood / bone marrow (EDTA, 2-5 ml) Frequency: 1x/week Test type: real time PCR on cDNA (WT1 ProfileQuant Kit, Ipsogen)