WT1 overexpression analysis

Lab Test name Contact Comments
UZA Mol. Diagn. WT1 overexpression analysis K. Vermeulen,
M. Le Mercier,
M-B Maes
Quantitative RT-PCR with the WT1 mRNA OneStep assay (Otsuka Pharmaceutical) on Lightcycler 480
Sample Type: Blood, Bone marrow
BELAC accreditation number 204-MED
Version date: 06/09/2022
UZ Gent WT1 overexpression analysis Barbara Denys,
Karl Vandepoele
Real-time PCR according to ELN guidelines (Cilloni et al., J Clin Oncol 2009)
BELAC accreditation number 087-MED
Version date: 16/03/2021
LHUB-ULB WT1 overexpression analysis Pierre Heimann/Laurent Dewispelaere/Pascale Cochaux/Hakim El Housni
SAMPLE TYPE: Peripheral Blood exclusively (EDTA, 5 mL)
TRANSPORT: room temperature, within 24h after sampling
FREQUENCY: upon request; max TAT: 4 weeks
TEST TYPE: One Step RT droplet digital PCR (Dewispelaere et al. JMD 2020). Laboratory developed test (LDT)
BELAC accreditation number 650-MED
Version date: 04/10/2021
IPG Gosselies WT1 overexpression analysis P. Vannuffel
C. De Rop
In house method base on Cilloni et al, Leukemia, 2002.
Quantitative RT-PCR.
Sample type: Blood and bone marrow.
BELAC accreditation number: 381-MED
Version date: 02/09/2022
AZ Delta WT1 overexpression analysis Elisabeth Moreau/Elke Boone
SAMPLE TYPE: Blood or bone marrow (1ml, EDTA)
TEST TYPE: real-time PCR in house protocol (Taqman gene expression assay for WT1 (exon 6/7) and ABL).
Version date: 05/12/2017
ZNA Middelheim WT1 overexpression analysis Pieter De Schouwer
In house method, normalised copy numbers / ABL.
Both Peripheral blood and bone marrow.
Lab specific reference values.
UZ Leuven MolPLUZ WT1 overexpression analysis Jolien De Bie
Sample type: blood / bone marrow (EDTA, 2-5 ml)
Frequency: 1x/week
Test type: real time PCR on cDNA (WT1 ProfileQuant Kit, Ipsogen)