Legionella pneumophila

Molecular detection of Legionella pneumophila
Lab Test name Contact Comments
AZ St-Jan Brugge - Laboratoriumgeneeskunde Legionella pneumophila Dr. M. Reynders / Patrick Descheemaeker
SAMPLE TYPE:
respiratoiry samples (min 550 µl)
FREQUENCY:
daily
TEST TYPE:
respiratoiry samples: TaqMan Array Card, in house real time PCR
Version date: 17/11/2017
UZA Mol. Diagn. Legionella pneumophila D. Ursi, K. Bergs, M. Michiels, V. Matheeussen
Sample type:
-respiratory specimens (swab, nasopharyngeal aspirate, sputum,...)
(at least 250 µl).

Frequency: 2 times a week

Test type: in house real-time PCR
UZ Brussel - Microbiology Legionella pneumophila Dr. Denis Piérard, Oriane Soetens
SAMPLE TYPE:
-BAL, sputum, bronchus aspirate, endotracheal aspirate, PSB and pleural liquid (min. 0.2 ml)
FREQUENCY: every weekday
TEST TYPE: in-house real-time PCR
TARGETS: mip (L. pneumophila), wzm (L. pneumophila sg 1) en 23S-5S intergenic spacer region (L. species)
Version date: 07/11/2017
Accredited:
OLVZ Aalst Legionella pneumophila Anne Vankeerberghen
Sample type:
Pharyngal swab
Respiratory sample (500 µl)

Frequency: Daily (except weekend)

Test type: In house real time PCR
Version date: 29/09/2017
CHU UCL NAMUR Legionella pneumophila pierre.bogaerts@uclouvain.be
Sample type:
BAL, sputum, bronchus aspirate, endotracheal aspirate, PSB and pleural liquid (min. 200 µL)
Test performed on Tuesday and Friday
In house real-time PCR
ZNA Middelheim Legionella pneumophila Walter Verstrepen
Sample type:
- respiratory specimens (swab, nasopharyngeal aspirate, sputum,...)
(at least 250 µl).

Frequency: every working day
Test type: in house real-time PCR
AZ St-Lucas Gent Legionella pneumophila Dr. A.M van den Abeele/Elke Vanlaere
SAMPLE TYPE:
-preferential: nasopharyngeal aspirate (min. 0.2 ml)
-alternative: BAL
FREQUENCY: 2x/week
TEST TYPE: Smartfinder (Pathofinder)
Jessa Hospital Legionella pneumophila dr Cartuyvels, Raymaekers Marijke
SAMPLE TYPE:
-nasopharyngeal aspirate or secretion
-nasopharyngeal e-swab (UTM or liquid amies)
-BA
FREQUENCY: Monday till Friday
TEST TYPE: in-house real time PCR
Accredited:
Imelda-Bonheiden Legionella pneumophila Dr. Johan Frans, Isabel Micalessi
Sample Type:
- NAP in UTM
- BAL
Frequency: Daily
Test Type: NxTAG Respiratoir Pathogeen Panel (MAGPIX, Luminex)
AZ Damiaan Oostende Legionella pneumophila Ben Vanmassenhove/ Anne-Sophie Hervent
SAMPLE TYPE: flocked swabs
FREQUENCY: 1-3/week
TEST TYPE: in-house Real Time PCR
Accredited:
GZA St-Augustinus Legionella pneumophila Philippe Van Lint
This PCR test is performed on respiratory samples (500µL).
Frequency: daily (Monday-Friday)

This respiratory panel screens for the following targets:
Viruses: Adenovirus, Bocavirus, Coronavirus, Enterovirus, Human metapneumovirus, (Para-)Influenza virus, Rhinovirus, RSV.
Bacteria: Bordetella (para-)pertussis, Bordetella holmesii, Chlamydophila pneumoniae, Legionella pneumophila, Mycoplasma pneumoniae.
UZ Leuven MolPLUZ Legionella pneumophila Katrien Lagrou
Remark: only available included in respiratory-panel-assay on swab, aspirate and BAL

Minimal sample volume: 1ml
Frequency: every day (Monday to Friday)
Test-type: in-house real-time PCR