Detection of mutations in the ABL kinase (ATP binding domain and P-loop). Resistance mechanism to Gleevec, Dasatinib, etc.
Lab
Test name
Contact
Comments
IPG Gosselies
Abelson (ABL1) mutation analysis
P. Vannuffel
C. De Rop
Mutation analysis of ABL1 tyrosine kinase domain
NGS AmpliSeq - IonTorrent
Sample type: Blood and bone marrow
BELAC accreditation number: 381-MED
Version date: 02/09/2022 Accredited:
LHUB-ULB
Abelson (ABL1) mutation analysis
Pierre Heimann/Barbara Dessars/Hakim El Housni
based on Branford S et al. protocol
PCR on cDNA followed by sequence analysis
UZ Leuven MolPLUZ
Abelson (ABL1) mutation analysis
Jolien De Bie
Sample type: blood / bone marrow (EDTA, 2-5 ml)
Frequency: 1x/week
Test type: digital droplet PCR (ddPCR) on DNA with PrimePCR ddPCR Mutation Assay (Bio-Rad) for BCR-ABL1 p.Q252H, p.Y253H, p.E255K, p.T315I, p.F317L, p.F359V
BELAC accreditation number 215-MED
Accredited:
NGS AmpliSeq - IonTorrent
Sample type: Blood and bone marrow
BELAC accreditation number: 381-MED
Version date: 02/09/2022
Accredited: