Calreticulin (CALR) mutation analysis

Lab Test name Contact Comments
UZ Brussel - Molecular Hematology Calreticulin (CALR) mutation analysis Marleen Bakkus/Christian Demanet
PCR on genomic DNA from blood or bone marrow followed by capillary electrophoresis. Ref: Klampfl et al. NEJM 2013; 369: 2379-90.
Version date: 26/09/2017
Accredited:
UZ Leuven MolPLUZ Calreticulin (CALR) mutation analysis Lucienne Michaux / Els Lierman
Sample type: blood / bone marrow (EDTA, 2-5 ml)
Frequency: 1x/week
Test type: PCR on DNA (ref: Klampf et al, NEJM 2013; exon 9)
Accredited:
AZ St-Jan Brugge - Laboratoriumgeneeskunde Calreticulin (CALR) mutation analysis dr. Helena Devos/Friedel Nollet, Ph.D.
method : PCR and capillary electropheresis. Sensitivity 5%
Version date: 04/11/2017
Accredited:
CHU Liège - Hemato Calreticulin (CALR) mutation analysis Dr Frédéric LAMBERT
3ml EDTA bone marrow.

Differential ET-PMF and very rare JAK2V617F-neg and exon 12 wt PV
Method : PCR and capillary electropheresis. Sequencing.
CUB Erasme - Medical Genetics Dpt Calreticulin (CALR) mutation analysis Pierre Heimann/Barbara Dessars/Hakim El Housni
Method: PCR and capillary electrophoresis. Sensitivity 5%
UZ Gent Calreticulin (CALR) mutation analysis Karl Vandepoele,
Joni Van Der Meulen,
Barbara Denys
In-house developed NGS panel to screen clinically relevant genes in MPN and MDS/MPN overlap:
CALR, CSF3R, JAK2, MPL, SETBP1 and SRSF2

Also available in a cost-effective mini-panel: JAK2, CALR and MPL
Version date: 18/08/2017
Accredited:
UZA Mol. Diagn. Calreticulin (CALR) mutation analysis K. Vermeulen, M-B Maes
AZ Delta Calreticulin (CALR) mutation analysis Geert Martens, Elke Boone
SAMPLE TYPE: Blood or bone marrow (1ml, EDTA)
FREQUENCY: upon request
TEST TYPE: PCR & Fragment analysis (exon 9 indel),
Version date: 05/12/2017
Accredited: