MYD88 L265P mutation analysis

Detection of MYD88 c.794T>C (p.Leu265Pro)

Lab	Test name	Contact	Comments
LHUB-ULB	MYD88 L265P mutation analysis	Pierre Heimann/Laurent Dewispelaere/Pascale Cochaux/Hakim El Housni	SAMPLE TYPE: Blood or bone marrow (EDTA, 1-2 mL) TRANSPORT: room temperature, within 24h after sampling FREQUENCY: variable; max TAT: 4 weeks TEST TYPE: MYD88 is analyzed together with CXCR4 and TP53 in an in-house developed NGS panels to refine diagnosis and prognosis of Waldenstrom macroglobulinemia. BELAC accreditation number 650-MED Version date: 04/10/2021 Accredited:
CHU Liège - Hemato	MYD88 L265P mutation analysis	Dr. Sc. Sabine FRANKE	3ml EDTA bone marrow or fresh lymph node biopsy. Diagnosis of Waldenstrom macroglobulinemia (WM) or lymphoplasmacytic leukemia (LPL); Work-up of DLBCL NHL ABC versus GC-like. BELAC accreditation number: 128-MED Accredited:
AZ St-Jan Brugge - Laboratoriumgeneeskunde	MYD88 L265P mutation analysis	dr. Helena Devos/Friedel Nollet, Ph.D.	real time PCR with blocking LNA primer (sensitivity 1% mutant DNA); DNA test Version date: 04/11/2017 Accredited:
UZ Gent	MYD88 L265P mutation analysis	Barbara Denys, Karl Vandepoele	Detection of the MYD88 c.794C>T mutation (L265P) qPCR with LNA blocker which allows selective amplification of the mutated allele BELAC accreditation number 087-MED FREQUENCY: 1x/week Version date: 16/03/2021 Accredited:
UZ Leuven MolPLUZ	MYD88 L265P mutation analysis	Jolien De Bie	Sample type: blood / bone marrow (EDTA, 2-5 ml) Frequency: 1x/week Test type: PCR on DNA, adapted from Treon et al., 2012. N Engl J Med BELAC accreditation number 215-MED Accredited:
UZ Brussel - Molecular Hematology	MYD88 L265P mutation analysis	Marleen Bakkus/Barbara Depreter	Detection of the MYD88 c.794C>T mutation (L265P). QPCR with LNA blocker, sensitivity 1% mutated allele. BELAC accreditation number: 141-MED Version date: 12/04/2023 Accredited:
IPG Gosselies	MYD88 L265P mutation analysis	P. Vannuffel C. De Rop	Digital Droplet PCR (ddPCR - Biorad) with PrimePCR ddPCR mutation assay for the p.L265P mutation in MYD88. Sample type: Blood and bone marrow. BELAC accreditation number: 381-MED. Version date: 02/09/2022 Accredited: