MYD88 L265P mutation analysis

Detection of MYD88 c.794T>C (p.Leu265Pro)
Lab Test name Contact Comments
LHUB-ULB MYD88 L265P mutation analysis Pierre Heimann/Laurent Dewispelaere/Pascale Cochaux/Hakim El Housni
SAMPLE TYPE: Blood or bone marrow (EDTA, 1-2 mL)
TRANSPORT: room temperature, within 24h after sampling
FREQUENCY: variable; max TAT: 4 weeks
TEST TYPE: MYD88 is analyzed together with CXCR4 and TP53 in an in-house developed NGS panels to refine diagnosis and prognosis of Waldenstrom macroglobulinemia.
BELAC accreditation number 650-MED
Version date: 04/10/2021
CHU Li├Ęge - Hemato MYD88 L265P mutation analysis Dr. Sc. Sabine FRANKE
3ml EDTA bone marrow or fresh lymph node biopsy.

Diagnosis of Waldenstrom macroglobulinemia (WM) or lymphoplasmacytic leukemia (LPL);

Work-up of DLBCL NHL ABC versus GC-like.

BELAC accreditation number: 128-MED
AZ St-Jan Brugge - Laboratoriumgeneeskunde MYD88 L265P mutation analysis dr. Helena Devos/Friedel Nollet, Ph.D.
real time PCR with blocking LNA primer (sensitivity 1% mutant DNA); DNA test
Version date: 04/11/2017
UZ Gent MYD88 L265P mutation analysis Barbara Denys,
Karl Vandepoele
Detection of the MYD88 c.794C>T mutation (L265P)
qPCR with LNA blocker which allows selective amplification of the mutated allele
BELAC accreditation number 087-MED

FREQUENCY: 1x/week
Version date: 16/03/2021
UZ Leuven MolPLUZ MYD88 L265P mutation analysis Jolien De Bie
Sample type: blood / bone marrow (EDTA, 2-5 ml)
Frequency: 1x/week
Test type: PCR on DNA, adapted from Treon et al., 2012. N Engl J Med
BELAC accreditation number 215-MED
UZ Brussel - Molecular Hematology MYD88 L265P mutation analysis Marleen Bakkus/Barbara Depreter
Detection of the MYD88 c.794C>T mutation (L265P).
QPCR with LNA blocker, sensitivity 1% mutated allele.
BELAC accreditation number: 141-MED
Version date: 12/04/2023
IPG Gosselies MYD88 L265P mutation analysis P. Vannuffel
C. De Rop
Digital Droplet PCR (ddPCR - Biorad) with PrimePCR ddPCR mutation assay for the p.L265P mutation in MYD88.
Sample type: Blood and bone marrow.
BELAC accreditation number: 381-MED.
Version date: 02/09/2022