Adenovirus

Qualitative and/or quantitative detection of adenovirus DNA
Lab Test name Contact Comments
UZ Leuven MolPLUZ Adenovirus Marc Van Ranst, Katrien Lagrou
Sample type:
- EDTA blood
- urine
- CSV
- faeces
- biopsy

Minimal sample volume: 1ml EDTA blood - 250 µl other sample types
Fequency: 2-5 times a week
Test-type: Quantitative in-house realtime PCR (500-5E+6 copies/ml)
Remark: also included in respiratory-panel-assay on swab, aspirate and BAL
OLVZ Aalst Adenovirus Anne Vankeerberghen
Sample type:
CSF (500 µl)
Pericard fluid (500 µl)
Respiratory sample (500 µl)
Pharyngal swab
Eye swab
Faeces

Frequency: Daily (except weekend)

Test type: In house real time PCR
Version date: 29/09/2017
Accredited:
CHU Saint-Pierre Adenovirus Reynders Marijke, Maaroufi Younes, Scheen Robert
Staaltypes: Respiratoire monsters (BAL, tracheobroncheolaire aspiratie, keelwissers, nasopharynxaspiraten, nasopharyngeale wissers), EDTA-bloedstalen, biopten, beenmerg, CSV, punctievochten.


Minimum staalvolume: 500 microliter voor de respiratoire stalen, 250 microliter voor de andere staaltypes.

Kwalitatieve in house real-time PCR
UZ Gent Adenovirus Elizaveta Padalko
Sample types: Respiratory samples (BAL, nasopharynxaspirate), EDTA-blood, serum.

Minimal sample volume: 500 microliter for respiratory samples, 250 microliter for othersample types.

Frequency: Wednesday(1x/2weeks)

Kwalitatieve in house real-time PCR
AZ St-Lucas Gent Adenovirus Dr A.M. van den Abeele/Elke Vanlaere
SAMPLE TYPE:
-preferential: nasopharyngeal aspirate (min. 0.2 ml)
-alternative: BAL
FREQUENCY: 2x/week
TEST TYPE: Smartfinder (Pathofinder)
LMMI Tilburg (NL) Adenovirus Dr. J.W.A. Rossen
Organisme: Adenovirus
Bepaling: DNA amplificatie
Methode: Real-time PCR (Taqman)
Soort bepaling: Kwalitatief
Typering: Nee
Frequentie: Dagelijks maandag t/m zaterdag
Commercieel: Nee
Externe QC: QCMD
Materiaal: EDTA-plasma, serum, liquor, bronchiaal secreet,
Bronchiolaire lavage (BAL), neus/keelwat, feces, oogvocht, sputum.
Jessa Hospital Adenovirus dr Cartuyvels, Raymaekers Marijke
SAMPLE TYPE:
-nasopharyngeal aspirate or secretion
-nasopharyngeal e-swab (UTM or liquid amies)
-BA
FREQUENCY: Monday till Friday
TEST TYPE: in-house real time PCR
Accredited:
Imelda-Bonheiden Adenovirus Dr. Johan Frans, Isabel Micalessi
Sample Type:
- NAP in UTM
- BAL
Frequency: Daily
Test Type: NxTAG Respiratoir Pathogeen Panel (MAGPIX, Luminex)
AZ St-Jan Brugge - Laboratoriumgeneeskunde Adenovirus Dr. M. Reynders, P. Descheemaeker
SAMPLE TYPE:
Urine, Stoelgang, placenta, vruchtwater, oogwissers, mucocutane wissers in UTM, weefsel, EDTA-Plasma, lumbaalvocht, BAL, respiratory sample
min 550 µl
FREQUENCY:
respiratoiry samples: daily
Gastro-intestinal samples: daily
other: 2x/week (Tuesday, Friday) 
TEST TYPE:
Respiratoiry samples: in house TaqMan array card
Gastro-intestinal samples: in house TaqMan array card
other: Rgene real time PCR quantitative
Version date: 17/11/2017
GZA St-Augustinus Adenovirus Philippe Van Lint
This PCR test is performed on respiratory samples (500µL).
Frequency: daily (Monday-Friday)

This respiratory panel screens for the following targets:
Viruses: Adenovirus, Bocavirus, Coronavirus, Enterovirus, Human metapneumovirus, (Para-)Influenza virus, Rhinovirus, RSV.
Bacteria: Bordetella (para-)pertussis, Bordetella holmesii, Chlamydophila pneumoniae, Legionella pneumophila, Mycoplasma pneumoniae.
Version date: 30/01/2018