Adenovirus

Qualitative and/or quantitative detection of adenovirus DNA
Lab Test name Contact Comments
UZ Leuven MolPLUZ Adenovirus Marc Van Ranst, Katrien Lagrou
Sample type:
- EDTA blood
- urine
- CSV
- faeces
- biopsy

Minimal sample volume: 1ml EDTA blood - 250 µl other sample types
Frequency: every weekday (csv: every day 7/7)
Test-type: Quantitative in-house realtime PCR (500-5E+6 copies/ml)
Remark: also included in respiratory-panel-assay on swab, aspirate and BAL
Accreditation BELAC: 215-MED
Accredited:
OLVZ Aalst Adenovirus Anne Vankeerberghen
Sample type:
CSF (500 µl)
Pericard fluid (500 µl)
Respiratory sample (500 µl)
Pharyngal swab
Eye swab
Faeces

Frequency: Daily (except weekend)

Test type: In house real time PCR
Version date: 06/05/2019
Accredited:
CHU Saint-Pierre Adenovirus Reynders Marijke, Maaroufi Younes, Scheen Robert
Staaltypes: Respiratoire monsters (BAL, tracheobroncheolaire aspiratie, keelwissers, nasopharynxaspiraten, nasopharyngeale wissers), EDTA-bloedstalen, biopten, beenmerg, CSV, punctievochten.


Minimum staalvolume: 500 microliter voor de respiratoire stalen, 250 microliter voor de andere staaltypes.

Kwalitatieve in house real-time PCR
UZ Gent Adenovirus Elizaveta Padalko
Sample types: Respiratory samples (BAL, nasopharynxaspirate), EDTA-blood, serum.

Minimal sample volume: 500 microliter for respiratory samples, 250 microliter for othersample types.

Frequency: Wednesday(1x/2weeks)

Kwalitatieve in house real-time PCR
AZ St-Lucas Gent Adenovirus Dr A.M. van den Abeele/Elke Vanlaere
SAMPLE TYPE:
-preferential: nasopharyngeal aspirate (min. 0.2 ml)
-alternative: BAL
FREQUENCY: 2x/week
TEST TYPE: Smartfinder (Pathofinder)
LMMI Tilburg (NL) Adenovirus Dr. J.W.A. Rossen
Organisme: Adenovirus
Bepaling: DNA amplificatie
Methode: Real-time PCR (Taqman)
Soort bepaling: Kwalitatief
Typering: Nee
Frequentie: Dagelijks maandag t/m zaterdag
Commercieel: Nee
Externe QC: QCMD
Materiaal: EDTA-plasma, serum, liquor, bronchiaal secreet,
Bronchiolaire lavage (BAL), neus/keelwat, feces, oogvocht, sputum.
Jessa Hospital Adenovirus dr Cartuyvels, Raymaekers Marijke
SAMPLE TYPE:
-nasopharyngeal aspirate or secretion
-nasopharyngeal e-swab (UTM or liquid amies)
-BA
FREQUENCY: Monday till Friday
TEST TYPE: in-house real time PCR
Accredited:
Imelda-Bonheiden Adenovirus Dr. Johan Frans, Isabel Micalessi
Sample Type:
- NAP in UTM
- BAL
Frequency: Daily
Test Type: NxTAG Respiratoir Pathogeen Panel (MAGPIX, Luminex)
AZ St-Jan Brugge - Laboratoriumgeneeskunde Adenovirus Dr. M. Reynders, P. Descheemaeker
SAMPLE TYPE:
Urine, Stoelgang, placenta, vruchtwater, oogwissers, mucocutane wissers in UTM, weefsel, EDTA-Plasma, lumbaalvocht, BAL, respiratory sample
min 550 µl
FREQUENCY:
respiratoiry samples: daily
Gastro-intestinal samples: daily
other: 2x/week (Tuesday, Friday) 
TEST TYPE:
Respiratoiry samples: in house TaqMan array card
Gastro-intestinal samples: in house TaqMan array card
other: Rgene real time PCR quantitative
Version date: 17/11/2017
AZ Damiaan Oostende Adenovirus Ben Vanmassenhove/ Anne-Sophie Hervent
Sample type: fecal swab (300 µl)
Frequency: daily (exept Sunday)
Allplex GI-Virus (Seegene)
Version date: 11/08/2023
Accredited:
GZA St-Augustinus Adenovirus Philippe Van Lint
This PCR test is performed on respiratory samples (500µL).
Frequency: daily (Monday-Friday)

The respiratory PCR is part of the following syndromic panel:
Seegene RV Master and Seegene Pneumobacter assay

Viruses
Influenza A virus
Influenza B virus
Human respiratory syncytial virus
Human metapneumovirus
Human adenovirus
Human rhinovirus
Human parainfluenza virus
SARS-CoV-2

Bacteria
Streptococcus pneumoniae
Legionella pneumophila
Haemophilus influenzae
Bordetella parapertussis
Mycoplasma pneumoniae
Bordetella pertussis
Chlamydophila pneumonia
--------------------------------
This PCR test is performed on faeces (or Copan FecalSwab).
Frequency: daily (Monday-Saturday)

The gastro PCR is part of the following syndromic panel:
Seegene GI-Virus, GI-Parasite and GI-EB SCreening assay

Parasites
Blastocystis hominis
Giardia lamblia
Dientamoeba fragilis
Entamoeba histolytica
Cyclospora cayetanensis
Cryptosporidium spp.

Bacteria
Y. enterocolitica
Shigella spp./EIEC
E. coli O157
C. difficile
STEC
Salmonella
Campylobacter spp.

Viruses
astrovirus
norovirus G2
adenovirus
sapovirus
norovirus G1
rotavirus
Version date: 30/01/2018
AZ Damiaan Oostende Adenovirus Ben Vanmassenhove/ Anne-Sophie Hervent
Sample type: respiratory sample
Frequency: 1-3/week
Allplex RV_Master (Seegene)
BELAC accreditation number 433-MED
Version date: 04/09/2023
Accredited:
Jan Yperman Ziekenhuis Adenovirus Bieke Decaesteker,
Stijn Jonckheere
Sample type: respiratory samples
Frequency: daily
Test Type: RT-PCR; QIAStat-Dx Respiratory SARS-CoV-2 panel (QIAStat, Qiagen)

The respiratory test also screens for the following targets:
Viruses: Bocavirus, Coronavirus, Enterovirus, Human metapneumovirus, (Para-)Influenza virus, Rhinovirus, RSV.
Bacteria: Bordetella (para-)pertussis, Legionella pneumophila, Mycoplasma pneumoniae.

Belac accreditation number 523-MED
Version date: 07/07/2022
Accredited:
AZ St-Lucas Brugge Adenovirus Dr Karel Maelegheer
Sample type: respiratory sample (300 µl)
Frequency: 1-3/week
Allplex Resp panel 2 Assay (Seegene)
Version date: 25/10/2022
AZ St-Lucas Brugge Adenovirus Dr Karel Maelegheer
Serotype 40/41
SAMPLE TYPE: Stool
FREQUENCY: 1-3/week
TEST TYPE:
GI Virus Assay Seegene (Starlet)
Version date: 26/10/2022