Enterovirus RNA

Qualitative and/or quantitative detection of enterovirus RNA
Lab Test name Contact Comments
UZA Mol. Diagn. Enterovirus RNA D. Ursi, K. Bergs, M. Michiels, V. Matheeussen
Sample type:
- cerebrospinal fluid
(at least 250 µl).

Frequency: 3 times a week

Test type: in house real-time PCR
UZ Brussel - Microbiology Enterovirus RNA Dr. Denis Piérard, Oriane Soetens
SAMPLE TYPE:
-CSF (min 0.2 ml)
FREQUENCY: 2 to 3 x/week
TEST TYPE: in-house real-time PCR
Version date: 26/09/2017
Accredited:
Jessa Hospital Enterovirus RNA dr. Cartuyvels, Raymaekers Marijke
SAMPLE TYPE:
-CSF
FREQUENCY: from Monday till Friday
TEST TYPE: GeneXpert
Accredited:
GZA St-Augustinus Enterovirus RNA Philippe Van Lint
This PCR test is performed on CSF (min 350µl) or on respiratory samples (500µL).
Frequency: daily (Monday-Friday)

The respiratory test also screens for the following targets:
Viruses: Adenovirus, Bocavirus, Coronavirus, Enterovirus, Human metapneumovirus, (Para-)Influenza virus, Rhinovirus, RSV.
Bacteria: Bordetella (para-)pertussis, Bordetella holmesii, Chlamydophila pneumoniae, Legionella pneumophila, Mycoplasma pneumoniae.
Accredited:
OLVZ Aalst Enterovirus RNA Anne Vankeerberghen
Sample type:
Faeces
Respiratory sample
CSF (500 µL)

Frequency: Daily (except weekend)
Test type: In house real time PCR
Version date: 29/09/2017
Accredited:
Ziekenhuis Oost-Limburg Enterovirus RNA Dr. Els Oris, Katrien Vandebroek
Sample type:
CSF

Frequency:
daily

Test type:
Xpert EV (GeneXpert, Cepheid)
CHU UCL NAMUR Enterovirus RNA pierre.bogaerts@uclouvain.be
Sample type:
CSF
Frequency:
Daily
Test type:
Xpert EV (GeneXpert, Cepheid)
AZ St-Lucas Gent Enterovirus RNA Dr. A.M van den Abeele/ Elke Vanlaere
Sample type: CSF

Frequency: daily

Test type: Xpert EV (GeneXpert, Cepheid)
UZ Leuven MolPLUZ Enterovirus RNA Marc Van Ranst, Katrien Lagrou
Sample type:
- CSV

Minimal sample volume: 250 µl
Frequency: every day (monday to friday)
Test type: In-house realtime PCR
Remark: also included in respiratory-panel-assay on swab, aspirate and BAL
Imelda-Bonheiden Enterovirus RNA Dr. Johan Frans, Isabel Micalessi
Sample type: CSF
Frequency: Daily
Test type: Meningitis-Encephalitis Panel (FilmArray, BioMerieux)
CHU Saint-Pierre Enterovirus RNA Reynders Marijke, Maaroufi Younes, Scheen Robert
Staaltypes: CSV, pericardvocht, andere punctievochten, biopten, stoelgang, respiratoire stalen (nasopharyngeale wissers en aspiraten, keelwissers, BAL, tracheobroncheolaire aspiraten).

Minimum staalvolume: 250 microliter voor niet-respiratoire stalen, 500 microliter voor respiratoire stalen.

Kwalitatieve in house real-time PCR
ZNA Middelheim Enterovirus RNA Walter Verstrepen
Sample type:
CSF
Pericard fluid
EDTA whole blood
(at least 250 µl)

Frequency: every working day
Test type: In house real time PCR
Labo Cliniques Sud Luxembourg Enterovirus RNA Nicolas Hougardy
SAMPLE TYPE:
- CSF (min 200µl)
FREQUENCY: 1x/week
TEST TYPE: in-house, real time PCR (5" non-coding region detection), LightCycler 2.0 (Roche)
LMMI Tilburg (NL) Enterovirus RNA Dr John WA Rossen
Organisme: Enterovirus
Bepaling: cDNA amplificatie
Methode: Real-time RT-PCR (Taqman)
Soort bepaling: Kwalitatief
Typering: Moleculair
Frequentie: Dagelijks maandag t/m zaterdag
Commercieel: Nee
Externe QC: QCMD
Materiaal: feces, liquor, neus/keelwat
AZ St-Jan Brugge - Laboratoriumgeneeskunde Enterovirus RNA Dr. M. Reynders / P. Descheemaeker
SAMPLE TYPE:
- Nasopharyngeal swab or aspirate, BAL (min. 1 ml)
- Stool
- CSV
FREQUENCY: Daily
TEST TYPE:
- TaqMan Array Card in house real time PCR
- In house real time PCR
Version date: 17/11/2017
Accredited: