Enterovirus RNA

Qualitative and/or quantitative detection of enterovirus RNA

Lab	Test name	Contact	Comments
UZA Mol. Diagn.	Enterovirus RNA	D. Ursi, K. Bergs, M. Michiels, V. Matheeussen	Sample type: - cerebrospinal fluid (at least 250 µl). Frequency: 3 times a week Test type: in house real-time PCR
Jessa Hospital	Enterovirus RNA	dr. Cartuyvels, Raymaekers Marijke	SAMPLE TYPE: -CSF FREQUENCY: from Monday till Friday TEST TYPE: GeneXpert Accredited:
GZA St-Augustinus	Enterovirus RNA	Philippe Van Lint	This PCR test is performed on CSV. Frequency: daily (Monday-Saturday) The CSV PCR is part of the following syndromic panel: QIAstat-Dx Meningitis/Encephalitis (ME) Panel Bacteria Streptococcus pneumoniae Neisseria meningitidis Streptococcus agalactiae Listeria monocytogenes Haemophilus influenzae Escherichia coli K1 Streptococcus pyogenes Mycoplasma pneumoniae Viruses Enterovirus Herpes simplex virus 1 Herpes simplex virus 2 Humaan parechovirus Humaan herpes virus 6 Varicella zoster virus Fungi Cryptococcus gattii/neoformans BELAC accreditation number 404-MED Accredited:
OLVZ Aalst	Enterovirus RNA	Anne Vankeerberghen	Sample type: Faeces Respiratory sample CSF (500 µL) Frequency: Daily (except weekend) Test type: In house real time PCR Version date: 06/05/2019 Accredited:
Ziekenhuis Oost-Limburg	Enterovirus RNA	Dr. Els Oris, Katrien Vandebroek	Sample type: CSF Frequency: daily Test type: Xpert EV (GeneXpert, Cepheid)
CHU UCL NAMUR	Enterovirus RNA	pierre.bogaerts@uclouvain.be	Sample type: CSF Frequency: Daily Test type: Xpert EV (GeneXpert, Cepheid)
AZ St-Lucas Gent	Enterovirus RNA	Dr. A.M van den Abeele/ Elke Vanlaere	Sample type: CSF Frequency: daily Test type: Xpert EV (GeneXpert, Cepheid)
UZ Leuven MolPLUZ	Enterovirus RNA	Marc Van Ranst, Katrien Lagrou	Sample type: - CSV Minimal sample volume: 250 µl Frequency: every day (7/7) Test type: In-house realtime PCR Remark: also included in respiratory-panel-assay on swab, aspirate and BAL Accreditation BELAC: 215-MED Accredited:
Imelda-Bonheiden	Enterovirus RNA	Dr. Johan Frans, Isabel Micalessi	Sample type: CSF Frequency: Daily Test type: Meningitis-Encephalitis Panel (FilmArray, BioMerieux)
CHU Saint-Pierre	Enterovirus RNA	Reynders Marijke, Maaroufi Younes, Scheen Robert	Staaltypes: CSV, pericardvocht, andere punctievochten, biopten, stoelgang, respiratoire stalen (nasopharyngeale wissers en aspiraten, keelwissers, BAL, tracheobroncheolaire aspiraten). Minimum staalvolume: 250 microliter voor niet-respiratoire stalen, 500 microliter voor respiratoire stalen. Kwalitatieve in house real-time PCR
ZNA Middelheim	Enterovirus RNA	Walter Verstrepen	Sample type: CSF Pericard fluid EDTA whole blood (at least 250 µl) Frequency: every working day Test type: In house real time PCR
Labo Cliniques Sud Luxembourg	Enterovirus RNA	Nicolas Hougardy	SAMPLE TYPE: - CSF (min 200µl) FREQUENCY: 1x/week TEST TYPE: in-house, real time PCR (5" non-coding region detection), LightCycler 2.0 (Roche)
LMMI Tilburg (NL)	Enterovirus RNA	Dr John WA Rossen	Organisme: Enterovirus Bepaling: cDNA amplificatie Methode: Real-time RT-PCR (Taqman) Soort bepaling: Kwalitatief Typering: Moleculair Frequentie: Dagelijks maandag t/m zaterdag Commercieel: Nee Externe QC: QCMD Materiaal: feces, liquor, neus/keelwat
AZ St-Jan Brugge - Laboratoriumgeneeskunde	Enterovirus RNA	Dr. M. Reynders / P. Descheemaeker	SAMPLE TYPE: - Nasopharyngeal swab or aspirate, BAL (min. 1 ml) - Stool - CSV FREQUENCY: Daily TEST TYPE: - TaqMan Array Card in house real time PCR - In house real time PCR Version date: 17/11/2017 Accredited:
Jan Yperman Ziekenhuis	Enterovirus RNA	Bieke Decaesteker Stijn Jonckheere	Sample type: CSF Frequency: daily Test Type: RT-PCR; QIAStat-Dx Meningitis/Encephalitis panel (QIAStat, Qiagen) The ME test also screens for the following targets: Viruses: Herpes simplex virus 1, Herpes Simplex virus 2, Human parechovirus, Human herpes virus 6, Varicella zoster virus Bacteria: Streptococcus pneumoniae, Neisseria meningitidis, Streptococcus agalactiae, Listeria monocytogenes, Haemophilus influenza, Escherichia coli K1, Streptococcus pyogenes, Mycoplasma pneumoniae Fungi: Cryptococcus neoformans/gattii Sample type: respiratory samples Frequency: daily Test Type: RT-PCR; QIAStat-Dx Respiratory SARS-CoV-2 panel (QIAStat, Qiagen) The respiratory test also screens for the following targets: Viruses: Adenovirus, Bocavirus, Coronavirus, Enterovirus, Human metapneumovirus, (Para-)Influenza virus, Rhinovirus, RSV. Bacteria: Bordetella (para-)pertussis, Legionella pneumophila, Mycoplasma pneumoniae. Belac accreditation number 523-MED Version date: 07/07/2022 Accredited:
AZ St-Lucas Brugge	Enterovirus RNA	Dr Karel Maelegheer	Sample type: respiratory sample (300 µl) Frequency: 1-3/week Allplex Resp panel 2 Assay (Seegene) Version date: 25/10/2022