Mycoplasma pneumoniae

Molecular detection of Mycoplasma pneumoniae

Lab	Test name	Contact	Comments
AZ St-Jan Brugge - Laboratoriumgeneeskunde	Mycoplasma pneumoniae	Dr. M. Reynders / Patrick Descheemaeker	SAMPLE TYPE: respiratoiry samples (min 550 µl) FREQUENCY: daily TEST TYPE: respiratoiry samples: TaqMan Array Card, in house real time PCR Version date: 17/11/2017
UZA Mol. Diagn.	Mycoplasma pneumoniae	D. Ursi, K. Bergs, M. Michiels, V. Matheeussen	Sample type: - respiratory specimens (swab, nasopharyngeal aspirate, sputum,...) - cerebrospinal fluid (at least 250 µl). Frequency: 2 times a week Test type: in house real-time PCR
OLVZ Aalst	Mycoplasma pneumoniae	Anne Vankeerberghen	Sample type: Pharyngal swab Respiratory sample (500 µl) Frequency: Daily (except weekend) Test type: In house real time PCR Version date: 06/05/2019 Accredited:
Ziekenhuis Oost-Limburg	Mycoplasma pneumoniae	Dr. Els Oris, Katrien Vandebroek	Sample type: nasopharyngeal aspirate Frequency: Daily (except weekend) Test type: In house real time PCR
CHU UCL NAMUR	Mycoplasma pneumoniae	pierre.bogaerts@uclouvain.be	Sample type: - respiratory specimens (swab, nasopharyngeal aspirate, sputum, BAL,...) - cerebrospinal fluid (at least 250 µl). Tests performed on Tuesday and Friday. In house real-time PCR.
UZ Leuven MolPLUZ	Mycoplasma pneumoniae	Katrien Lagrou Emmanuel Andre	Sample type: - respiratory swab - respiratory samples Minimal sample volume: 1ml Frequency: every weekday Test type: In-house realtime PCR Remark: also included in respiratory-panel-assay on swab, aspirate and BAL Accreditation BELAC: 215-MED Accredited:
CHU Saint-Pierre	Mycoplasma pneumoniae	Reynders Marijke, Maaroufi Younes, Scheen Robert	Staaltypes: respiratoire stalen (BAL, sputum, tracheobroncheolaire aspiraten, nasopharynxaspiraten) Minimum staalvolume: 250 microliter Kwalitatieve in house real-time PCR
ZNA Middelheim	Mycoplasma pneumoniae	Walter Verstrepen	Sample type: - respiratory specimens (swab, nasopharyngeal aspirate, sputum,...) - cerebrospinal fluid (at least 250 µl). Frequency: every working day Test type: in house real-time PCR
GZA St-Augustinus	Mycoplasma pneumoniae	Philippe Van Lint	This PCR test is performed on respiratory samples (500µL). Frequency: daily (Monday-Friday) The respiratory PCR is part of the following syndromic panel: Seegene RV Master and Seegene Pneumobacter assay Viruses Influenza A virus Influenza B virus Human respiratory syncytial virus Human metapneumovirus Human adenovirus Human rhinovirus Human parainfluenza virus SARS-CoV-2 Bacteria Streptococcus pneumoniae Legionella pneumophila Haemophilus influenzae Bordetella parapertussis Mycoplasma pneumoniae Bordetella pertussis Chlamydophila pneumonia ------------------------------------- This PCR test is performed on CSV. Frequency: daily (Monday-Saturday) The CSV PCR is part of the following syndromic panel: QIAstat-Dx Meningitis/Encephalitis (ME) Panel Bacteria Streptococcus pneumoniae Neisseria meningitidis Streptococcus agalactiae Listeria monocytogenes Haemophilus influenzae Escherichia coli K1 Streptococcus pyogenes Mycoplasma pneumoniae Viruses Enterovirus Herpes simplex virus 1 Herpes simplex virus 2 Humaan parechovirus Humaan herpes virus 6 Varicella zoster virus Fungi Cryptococcus gattii/neoformans Accredited:
Labo Cliniques Sud Luxembourg	Mycoplasma pneumoniae	Nicolas Hougardy	SAMPLE TYPE: - BAL (min 1ml) - Swabs FREQUENCY: 1x/day (except weekend) TEST TYPE: "Mycoplasma pneumoniae Chlamydophila pneumoniae Real-Time PCR" (Diagenode), LightCycler (Roche)
Imelda-Bonheiden	Mycoplasma pneumoniae	Dr. Johan Frans, Isabel Micalessi	Sample Type: - NAP in UTM - BAL Frequency: Daily Test Type: NxTAG Respiratoir Pathogeen Panel (MAGPIX, Luminex)
AZ St-Lucas Gent	Mycoplasma pneumoniae	Dr. A.M van den Abeele/Elke Vanlaere	SAMPLE TYPE: -preferential: nasopharyngeal aspirate (min. 0.2 ml) -alternative: BAL FREQUENCY: 2x/week TEST TYPE: Smartfinder (Pathofinder)
Jessa Hospital	Mycoplasma pneumoniae	dr Cartuyvels, Raymaekers Marijke	SAMPLE TYPE: -nasopharyngeal aspirate or secretion -nasopharyngeal e-swab (UTM or liquid amies) -BA FREQUENCY: Monday till Friday TEST TYPE: in-house real time PCR Accredited:
AZ Damiaan Oostende	Mycoplasma pneumoniae	Ben Vanmassenhove/ Anne-Sophie Hervent	SAMPLE TYPE: flocked swabs FREQUENCY: 1-3/week TEST TYPE: Allplex RP4 (Seegene) BELAC accreditation number 433-MED Version date: 17/05/2021 Accredited:
Jan Yperman Ziekenhuis	Mycoplasma pneumoniae	Bieke Decaesteker, Stijn Jonckheere	Sample Type: Cerebrospinal fluid Frequency: Daily Test Type: RT-PCR; QIAStat-Dx Meningitis/Encephalitis panel (QIAStat, Qiagen) The ME test also screens for the following targets: Viruses: Enterovirus, Herpes Simplex virus 1, Herpes Simplex virus 2, Human parechovirus, Human herpes virus 6, Varicella zoster virus Bacteria: Streptococcus pneumoniae, Neisseria meningitidis, Streptococcus agalactiae, Listeria monocytogenes, Haemophilus influenza, Escherichia coli K1, Streptococcus pyogenes Fungi: Cryptococcus neoformans/gattii Sample type: respiratory samples Frequency: daily Test Type: RT-PCR; QIAStat-Dx Respiratory SARS-CoV-2 panel (QIAStat, Qiagen) The respiratory test also screens for the following targets: Viruses: Adenovirus, Bocavirus, Coronavirus, Enterovirus, Human metapneumovirus, (Para-)Influenza virus, Rhinovirus, RSV. Bacteria: Bordetella (para-)pertussis, Legionella pneumophila Belac accreditation number 523-MED Version date: 07/07/2022 Accredited:
AZ St-Lucas Brugge	Mycoplasma pneumoniae	Dr Karel Maelegheer	Sample type: respiratory sample (300 µl) Frequency: 1-3/week Allplex PneumoBacter Assay (Seegene) Version date: 25/10/2022