DNMT3A mutation analysis

Lab Test name Contact Comments
UZ Leuven MolPLUZ DNMT3A mutation analysis Lucienne Michaux / Els Lierman
Sample type: blood / bone marrow (EDTA, 2-5 ml)
Frequency: 1x/week
Test type: PCR on DNA (exon 23) + Sanger sequencing
(ref: Ley et al, NEJM 2010)
CHU Liège - Hemato DNMT3A mutation analysis Dr Frédéric LAMBERT
3ml EDTA anticoagulated bone marrow with > 20% myeloid blasts.

Prognostic marker de novo AML with normal karyotype.

Sanger or NGS sequencing.
AZ St-Jan Brugge - Laboratoriumgeneeskunde DNMT3A mutation analysis dr. Helena Devos/Friedel Nollet, Ph.D.
Method: Sanger sequencing of DNMT3A gene (exon-23, p.Val867 to p.Val912)
Version date: 04/11/2017