FLT3 D835 (FLT3-TKD) mutation analysis

FLT3-TKD (D835) mutation analysis
Lab Test name Contact Comments
UZ Leuven MolPLUZ FLT3 D835 (FLT3-TKD) mutation analysis Jolien De Bie
Sample type: blood / bone marrow (EDTA, 2-5 ml)
Frequency: 1x/week
Test type: PCR on DNA, adapted from Abu-Duhier et al, Br J Haematol 2001.
BELAC accreditation number 215-MED
UZA Mol. Diagn. FLT3 D835 (FLT3-TKD) mutation analysis K. Vermeulen,
M. Le Mercier,
M-B Maes
PCR on DNA followed by fragment analysis (adapted from Murphy et al., 2003).
TAT: max 8 days
Sample Type: Blood, Bone marrow
BELAC accreditation number 204-MED
Version date: 06/09/2022
UZ Gent FLT3 D835 (FLT3-TKD) mutation analysis Barbara Denys,
Karl Vandepoele
Quick, simultaneous detection with NPM1, FLT3-ITD and CEBPA (capillary electrophoresis)
DNA test
TAT: 8 days
BELAC accreditation number 087-MED
Version date: 16/03/2021
UZ Brussel - Molecular Hematology FLT3 D835 (FLT3-TKD) mutation analysis Marleen Bakkus/Barbara Depreter
PCR on DNA followed by fragment analysis (adapted from Murphy et al., 2003).
Sample Type: EDTA-blood (5ml) or EDTA-bone marrow (2ml)
BELAC accreditation number: 141-MED
Version date: 12/04/2023
IPG Gosselies FLT3 D835 (FLT3-TKD) mutation analysis P. Vannuffel
C. De Rop
AML diagnostic: In-house method based on PrimePCR ddPCR assay for WT FLT3 D835- I836 with restriction enzyme by digital droplet PCR (Biorad)

AML follow-up: Digital Droplet PCR (ddPCR - Biorad) with PrimePCR ddPCR mutation assay for a mutation at codon D835 and I836 of FLT3.

Sample type: Blood and bone marrow
BELAC accreditation number: 381-MED
Version date: 28/11/2023