IgH hypermutation is a prognostic marker for patients with chronic lymphocytic leukemia (CLL)
Lab
Test name
Contact
Comments
IPG Gosselies
IgH hypermutation analysis
P. Vannuffel
C. De Rop
In house method based on Fais et al, J. Clin. Invest, 1998 and Gregory at al, Journal of Immunology, 2003.
Sanger sequencing
Sample type: Blood and bone marrow
Version date: 02/09/2022 Accredited:
UZ Brussel - Molecular Hematology
IgH hypermutation analysis
Marleen Bakkus/Barbara Depreter
Prognostic parameter in CLL
Sample: DNA or 1 ml of EDTA bone marrow or blood
Assay: lymphotrack NGS kit (Invivoscribe)
Frequency: 1x/week
BELAC accreditation number: 141-MED
Version date: 12/04/2023 Accredited:
SAMPLE TYPE:
-Blood or bone marrow (1ml, EDTA)
-Biopsy (fresh or FFPE)
TRANSPORT: room temperature
FREQUENCY: 1x/week
TEST TYPE: PCR & Sequencing, in house method from van Dongen et al,Leukemia, 2003, Biomed-2
BELAC Accreditation number 275-MED
Version date: 25/05/2021 Accredited:
LHUB-ULB
IgH hypermutation analysis
Pierre Heimann/Laurent Dewispelaere/Pascale Cochaux/Hakim El Housni
SAMPLE TYPE: Peripheral blood or bone marrow (EDTA, 1-2 mL); biopsy (fresh)
TRANSPORT: room temperature, within 24h after sampling
FREQUENCY: upon request; max TAT: 5 weeks
TEST TYPE: Massive Parallel Sequencing (LymphoTrack SHM kit; INVIVOSCRIBE - CE IVD)
BELAC accreditation number 650-MED
Version date: 04/10/2021
AZ Delta
IgH hypermutation analysis
Elisabeth Moreau/Elke Boone
SAMPLE TYPE:
-Blood or bone marrow
-Biopsy
FREQUENCY: on request
TEST TYPE: in house method VH-Leader (or Biomed FR1) PCR amplification followed by sequencing (ERIC recommendations)
Version date: 05/12/2017 Accredited:
ZNA Middelheim
IgH hypermutation analysis
Pieter De Schouwer
sample type:bone marrow ro EDTA blood
frequency:once a week
UZ Leuven MolPLUZ
IgH hypermutation analysis
Jolien De Bie
Sample type: blood / bone marrow (EDTA, 2-5 ml)
Frequency: 1x/week
Test type: PCR on DNA (can also be performed on RNA), based on Aubin et al, Leukemia 1995 and Campbell et al, Molecular Immunology 1992.
BELAC accreditation number 215-MED
Accredited:
AZ St-Jan Brugge - Laboratoriumgeneeskunde
IgH hypermutation analysis
dr. Helena Devos / Friedel Nollet, Ph.D.
VH-family leader and FR1 primer PCR + Sanger sequencing
Version date: 24/10/2019 Accredited:
Sanger sequencing
Sample type: Blood and bone marrow
Version date: 02/09/2022
Accredited: