IgH hypermutation analysis

IgH hypermutation is a prognostic marker for patients with chronic lymphocytic leukemia (CLL)

Lab	Test name	Contact	Comments
IPG Gosselies	IgH hypermutation analysis	P. Vannuffel C. De Rop	In house method based on Fais et al, J. Clin. Invest, 1998 and Gregory at al, Journal of Immunology, 2003. Sanger sequencing Sample type: Blood and bone marrow Version date: 02/09/2022 Accredited:
UZ Brussel - Molecular Hematology	IgH hypermutation analysis	Marleen Bakkus/Barbara Depreter	Prognostic parameter in CLL Sample: DNA or 1 ml of EDTA bone marrow or blood Assay: lymphotrack NGS kit (Invivoscribe) Frequency: 1x/week BELAC accreditation number: 141-MED Version date: 12/04/2023 Accredited:
CHU Liège - Hemato	IgH hypermutation analysis	Dr Sc. Sabine Franke	Commercial assay: IgH Somatic Hypermutation Assay, InVivoScribe Technologies. BELAC accreditation number: 128-MED Accredited:
Jessa Hospital	IgH hypermutation analysis	Brigitte Maes/Ellen Geerdens	SAMPLE TYPE: -Blood or bone marrow (1ml, EDTA) -Biopsy (fresh or FFPE) TRANSPORT: room temperature FREQUENCY: 1x/week TEST TYPE: PCR & Sequencing, in house method from van Dongen et al,Leukemia, 2003, Biomed-2 BELAC Accreditation number 275-MED Version date: 25/05/2021 Accredited:
LHUB-ULB	IgH hypermutation analysis	Pierre Heimann/Laurent Dewispelaere/Pascale Cochaux/Hakim El Housni	SAMPLE TYPE: Peripheral blood or bone marrow (EDTA, 1-2 mL); biopsy (fresh) TRANSPORT: room temperature, within 24h after sampling FREQUENCY: upon request; max TAT: 5 weeks TEST TYPE: Massive Parallel Sequencing (LymphoTrack SHM kit; INVIVOSCRIBE - CE IVD) BELAC accreditation number 650-MED Version date: 04/10/2021
AZ Delta	IgH hypermutation analysis	Elisabeth Moreau/Elke Boone	SAMPLE TYPE: -Blood or bone marrow -Biopsy FREQUENCY: on request TEST TYPE: in house method VH-Leader (or Biomed FR1) PCR amplification followed by sequencing (ERIC recommendations) Version date: 05/12/2017 Accredited:
ZNA Middelheim	IgH hypermutation analysis	Pieter De Schouwer	sample type:bone marrow ro EDTA blood frequency:once a week
UZ Leuven MolPLUZ	IgH hypermutation analysis	Jolien De Bie	Sample type: blood / bone marrow (EDTA, 2-5 ml) Frequency: 1x/week Test type: PCR on DNA (can also be performed on RNA), based on Aubin et al, Leukemia 1995 and Campbell et al, Molecular Immunology 1992. BELAC accreditation number 215-MED Accredited:
AZ St-Jan Brugge - Laboratoriumgeneeskunde	IgH hypermutation analysis	dr. Helena Devos / Friedel Nollet, Ph.D.	VH-family leader and FR1 primer PCR + Sanger sequencing Version date: 24/10/2019 Accredited: