IgH hypermutation analysis

IgH hypermutation is a prognostic marker for patients with chronic lymphocytic leukemia (CLL)

Lab	Test name	Contact	Comments
IPG Gosselies	IgH hypermutation analysis
UZ Brussel - Molecular Hematology	IgH hypermutation analysis	Marleen Bakkus/Christian Demanet	Prognostic parameter in CLL Sample: DNA or 1 ml of EDTA bone marrow or blood Assay: lymphotrack NGS kit (Invivoscribe) Frequency: 1x/month Version date: 26/09/2017 Accredited:
CHU Liège - Hemato	IgH hypermutation analysis	Dr Sc. Sabine Franke	Commercial assay: IgH Somatic Hypermutation Assay, InVivoScribe Technologies.
Jessa Hospital	IgH hypermutation analysis	Dr. Brigitte Maes, Femke Hillen	SAMPLE TYPE: -Blood or bone marrow (1ml, EDTA) -Biopsy (fresh or FFPE) TRANSPORT: room temperature FREQUENCY: 1x/week TEST TYPE: PCR & Sequencing, in house method from van Dongen et al,Leukemia, 2003, Biomed-2
CUB Erasme - Medical Genetics Dpt	IgH hypermutation analysis	Pierre Heimann/Barbara Dessars/Hakim El Housni	IGH Somatic Hypermutation Assay v2.0 (Invivoscribe) PCR and capillary electrophoresis
AZ Delta	IgH hypermutation analysis	Elisabeth Moreau/Elke Boone	SAMPLE TYPE: -Blood or bone marrow -Biopsy FREQUENCY: on request TEST TYPE: in house method VH-Leader (or Biomed FR1) PCR amplification followed by sequencing (ERIC recommendations) Version date: 05/12/2017 Accredited:
ZNA Middelheim	IgH hypermutation analysis	Pieter De Schouwer	sample type:bone marrow ro EDTA blood frequency:once a week
UZ Leuven MolPLUZ	IgH hypermutation analysis	Lucienne Michaux / Els Lierman	Sample type: blood / bone marrow (EDTA, 2-5 ml) Frequency: 1x/week Test type: PCR on DNA (can also be performed on RNA), based on Aubin et al, Leukemia 1995 and Campbell et al, Molecular Immunology 1992. Accredited:
AZ St-Jan Brugge - Laboratoriumgeneeskunde	IgH hypermutation analysis	dr. Helena Devos / Friedel Nollet, Ph.D.	VH-family FR1 primer PCR + Sanger sequencing Version date: 15/03/2018 Accredited: