MPL mutation analysis

Lab	Test name	Contact	Comments
AZ St-Jan Brugge - Laboratoriumgeneeskunde	MPL mutation analysis	dr. Helena Devos/Friedel Nollet, Ph.D.	PCR amplification (on DNA) + DNA sequencing for detection of S505 and W515 mutations in the MPL gene. Version date: 04/11/2017 Accredited:
CHU Liège - Hemato	MPL mutation analysis	Dr Frédéric LAMBERT	3 ml EDTA anticoagulated bone marrow. Inherited/familial thrombocytosis. BELAC accreditation number: 128-MED 33 Nomenclature Accredited:
UZ Leuven MolPLUZ	MPL mutation analysis	Jolien De Bie	Sample type: blood / bone marrow (EDTA, 2-5 ml) Frequency: 1x/week Test type: PCR on DNA (exon 10) BELAC accreditation number 215-MED Accredited:
UZ Gent	MPL mutation analysis	Karl Vandepoele, Joni Van Der Meulen, Barbara Denys	In-house developed NGS panel to screen clinically relevant genes in MPN and MDS/MPN overlap: CALR, JAK2 and MPL. BELAC accreditation number 087-MED These genes are also present in the larger 68 gene panel. Also available in a cost-effective mini-panel: JAK2, CALR and MPL Version date: 16/03/2021 Accredited:
LHUB-ULB	MPL mutation analysis	Pierre Heimann/Laurent Dewispelaere/Pascale Cochaux/Hakim El Housni	SAMPLE TYPE: Peripheral Blood (5mL) or bone marrow (EDTA, 1-2 mL) TRANSPORT: room temperature, within 24h after sampling FREQUENCY: variable: max TAT: 4 weeks TEST TYPE: MPL is analyzed in several in-house developed NGS panels designed to refine diagnosis and prognosis of MPN and MDS/MPN overlap syndrome. BELAC accreditation number 650-MED Version date: 04/10/2021 Accredited: