NPM1 mutation analysis

NPM1 (nucleophosmin) mutation analysis
Lab Test name Contact Comments
AZ St-Jan Brugge - Laboratoriumgeneeskunde NPM1 mutation analysis dr. Helena Devos/Friedel Nollet, Ph.D.
Only at diagnosis or relapse: DNA test, PCR and capillary electrophoresis. Multiplex assay with Flt3-ITD and CEBPalpha mutation analysis. Consensus primers from working group MolecularDiagnostics.be. Sanger DNA-sequencing is used to detect type of mutation.
Version date: 04/11/2017
Accredited:
IPG Gosselies NPM1 mutation analysis
UZ Gent NPM1 mutation analysis Barbara Denys,
Karl Vandepoele
Simultaneous detection with FLT3-ITD, FLT3-TKD and CEBPA (capillary electrophoresis)
DNA test

NPM1 is also present in our NGS panel for myeloid malignancies.
Version date: 25/09/2018
Accredited:
Jessa Hospital NPM1 mutation analysis Dr. Brigitte Maes, Femke Hillen
SAMPLE TYPE:
Blood or bone marrow (1ml, EDTA)
TRANSPORT: room temperature
FREQUENCY: 1x/week
TEST TYPE: Only at diagnosis or relapse: DNA test, PCR and capillary electrophoresis. Multiplex assay with Flt3-ITD mutation analysis. Consensus primers from working group MolecularDiagnostics.be.
UZ Leuven MolPLUZ NPM1 mutation analysis Lucienne Michaux / Els Lierman
Sample type: blood / bone marrow (EDTA, 2-5 ml)
Frequency: 1x/week
Test type: PCR on cDNA, adapted from Noguera et al, Leukemia 2002.
Accredited:
CUB Erasme - Medical Genetics Dpt NPM1 mutation analysis Pierre Heimann/Barbara Dessars/Hakim El Housni
PCR and capillary electrophoresis

Analytical sensitivity: 1%
UZ Brussel - Molecular Hematology NPM1 mutation analysis Marleen Bakkus/Christian Demanet
Only at diagnosis or relapse: DNA test, PCR and capillary electrphoresis. Multiplex assay with Flt3-ITD and CEBPalpha mutation analysis. Consensus primers from working group MolecularDiagnostics.be.
Version date: 26/09/2017
Accredited:
AZ Delta NPM1 mutation analysis Geert Martens, Elke Boone
SAMPLE TYPE: blood or bone marrow (1 ml)
FREQUENCY: upon request, at diagnosis or relapse
TEST TYPE: DNA test, PCR and capillary electrophoresis. Multiplex assay with CEBPA and FLT3-ITD mutation analysis. Consensus primers from working group MolecularDiagnostics.be.
Version date: 05/12/2017
Accredited: