chimerism analysis

chimerism analysis for detection of %donor after allogeneic transplantation
Lab Test name Contact Comments
AZ St-Jan Brugge - Laboratoriumgeneeskunde chimerism analysis dr. Helena Devos/Friedel Nollet, Ph.D.
Powerplex16 kit to detect %donor chimerism (range 5%-95%); DNA-test
Reference samples from donor and recipient (before transplant) are needed.
Version date: 04/11/2017
Accredited:
IPG Gosselies chimerism analysis
UZ Brussel - Molecular Hematology chimerism analysis Marleen Bakkus/Christian Demanet
Powerplex16 kit to detect %donor chimerism (range 5%-95%); DNA-test
Reference samples from donor and recipient (before transplant) are needed.
Version date: 26/09/2017
Accredited:
CHU Li├Ęge - Hemato chimerism analysis Dr Sc E. Castermans & Dr V. Dideberg
LHUB-ULB chimerism analysis Pierre Heimann/Laurent Dewispelaere/Pascale Cochaux/Hakim El Housni
SAMPLE TYPE: Peripheral Blood (5mL) or bone marrow (EDTA, 1-2 mL); CD3+
TRANSPORT: room temperature, within 24h after sampling
FREQUENCY: upon request; max TAT: 4 weeks
TEST TYPE: NGM kit; Detect % of donor chimerism (range 5-95%).
Donor and recipient (BEFORE transplant) are needed
Laboratory developed test (LDT)
BELAC accreditation number 650-MED
Version date: 04/10/2021
Accredited:
UZ Gent - CMGG chimerism analysis kathleen claes
ZNA Middelheim chimerism analysis Pieter De Schouwer
Sample type: bone marrow or EDTA blood
Frequency: once a week
Test type: commercial kit (AmpFISTR Profiler Plus Kit)
UZ Leuven MolPLUZ chimerism analysis Jolien De Bie
Sample type: blood / bone marrow (EDTA, 2-5 ml), stem cells, tissue, T-cells
Frequency: 1x/week
Test type: PCR on DNA using the Powerplex16 kit (Promega). Donor and receptor profiles prior to transplantation are needed for chimerism interpretation post-transplantation.
BELAC accreditation number 215-MED
Accredited: