IgH clonality analysis

Detection of IgH monoclonality
Lab Test name Contact Comments
AZ St-Jan Brugge - Laboratoriumgeneeskunde IgH clonality analysis dr. Helena Devos/Friedel Nollet, Ph.D.
PCR on DNA followed by capillary electrophoresis.
FR1-JH and FR3-JH primers from BIOMED-2 consortium, Van Dongen et al. Leukemia (2003)
Version date: 04/11/2017
UZA Mol. Diagn. IgH clonality analysis K. Vermeulen,
M. Le Mercier,
M-B Maes
PCR on DNA followed by fragment analysis. Biomed-2 protocol targets FR1, FR2, FR3 and DJ (Van Dongen et al., Leukemia 2003)
Sample Type: Blood, Bone marrow, Biopt (Fresh or FFPE), CSF (or other fluids).
BELAC accreditation number 204-MED
Version date: 06/09/2022
IPG Gosselies IgH clonality analysis P. Vannuffel
C. De Rop
In house method based on Nizet et al, Blood, 1993.
Microfluidic technology
Sample type: Blood and bone marrow.
BELAC accreditation number: 381-MED
Version date: 02/09/2022
UZ Gent IgH clonality analysis Barbara Denys
Karl Vandepoele
BIOMED-2 protocol according to van Dongen et al, Leukemia 2003;
BELAC accreditation number 087-MED
Version date: 16/03/2021
UZ Brussel - Molecular Hematology IgH clonality analysis Marleen Bakkus/Barbara Depreter
Fluorescent PCR on DNA followed by Fragment analysis, in-house and Biomed-2 protocol targets FR1, FR2, FR3 and DJ (van Dongen et al, Leukemia, 2003)
BELAC accreditation number: 141-MED
Version date: 12/04/2023
CHU Liège - Hemato IgH clonality analysis Dr Sc Sabine Franke
Commercial assay: IdentitiClone IgH Gene Clonality Assay, InVivoScribe Technologies.

BELAC accreditation number: 128-MED
Jessa Hospital IgH clonality analysis Dr. Brigitte Maes, Ellen Geerdens
-Blood or bone marrow (1ml, EDTA)
-Biopsy (fresh or FFPE)
-CSF (or other fluids, 1ml)
TRANSPORT: room temperature
TEST TYPE:PCR & Fragment analysis, in house method from van Dongen et al,Leukemia, 2003, Biomed-2
LHUB-ULB IgH clonality analysis Pierre Heimann/Laurent Dewispelaere/Pascale Cochaux/Hakim El Housni
SAMPLE TYPE: Blood or bone marrow (EDTA, 1-2 mL); biopsy; PBSC
TRANSPORT: room temperature, within 24h after sampling
FREQUENCY: 1/2weeks; max TAT: 5 weeks
TEST TYPE: IdentiClone IgH Gene Clonality, Invivoscribe Technologies; IVD-CE labelled kit
BELAC accreditation number 650-MED
Version date: 04/10/2021
OLVZ Aalst IgH clonality analysis Anne Vankeerberghen
Sample type:
EDTA-Blood or bone marrow (500 µl)
Gland tissue
Frequency: 1x in 2 weeks
Test type: Fragment analysis (Biomed primers)
Version date: 06/05/2019
ZNA Middelheim IgH clonality analysis Evelien Heylen
-Blood or bone marrow (1ml, EDTA)
-Biopsy (fresh or FFPE)
-CSF (or other fluids, 1ml)

FREQUENCY: 2x/week
TEST TYPE: Biomed-2 PCR according to van Dongen et al, Leukemia (2003)

Performed in conjunction with IgK and IgL clonality analysis
AZ Delta IgH clonality analysis Elisabeth Moreau/Elke Boone
-Blood or bone marrow (1ml, EDTA)
-Biopsy (fresh or FFPE)
-CSF (or other fluids, 1ml)
TEST TYPE: PCR & Fragment analysis, Biomed-2 protocol targets FR1, FR2, FR3 and DJ (van Dongen et al,Leukemia, 2003)
Version date: 05/12/2017
Labo Cliniques Sud Luxembourg IgH clonality analysis Nicolas Hougardy
- Blood (EDTA) (min 200µL)
- Bone marrow (EDTA) (min 2,5ml)
FREQUENCY: 1x/month
TEST TYPE: “ Identiclone IGH gene clonality assay”(Invivoscribe), Experion Electrophoresis System (BioRad)
UZ Leuven MolPLUZ IgH clonality analysis Jolien De Bie
Sample type: blood / bone marrow (EDTA, 2-5 ml) / tissue (fresh or FFPE)
Frequency: 1x/week
Test type: PCR on DNA, based on van Dongen et al., Leukemia 2003 (BIOMED-2 protocol).
BELAC accreditation number 215-MED