IgH clonality analysis

Detection of IgH monoclonality

Lab	Test name	Contact	Comments
AZ St-Jan Brugge - Laboratoriumgeneeskunde	IgH clonality analysis	dr. Helena Devos/Friedel Nollet, Ph.D.	PCR on DNA followed by capillary electrophoresis. FR1-JH and FR3-JH primers from BIOMED-2 consortium, Van Dongen et al. Leukemia (2003) Version date: 04/11/2017 Accredited:
UZA Mol. Diagn.	IgH clonality analysis	K. Vermeulen, M. Le Mercier, M-B Maes	PCR on DNA followed by fragment analysis. Biomed-2 protocol targets FR1, FR2, FR3 and DJ (Van Dongen et al., Leukemia 2003) Sample Type: Blood, Bone marrow, Biopt (Fresh or FFPE), CSF (or other fluids). BELAC accreditation number 204-MED Version date: 06/09/2022 Accredited:
IPG Gosselies	IgH clonality analysis	P. Vannuffel C. De Rop	In house method based on Nizet et al, Blood, 1993. Microfluidic technology Sample type: Blood and bone marrow. BELAC accreditation number: 381-MED Version date: 02/09/2022 Accredited:
UZ Gent	IgH clonality analysis	Barbara Denys Karl Vandepoele	BIOMED-2 protocol according to van Dongen et al, Leukemia 2003; DNA-test BELAC accreditation number 087-MED Version date: 16/03/2021 Accredited:
UZ Brussel - Molecular Hematology	IgH clonality analysis	Marleen Bakkus/Barbara Depreter	Fluorescent PCR on DNA followed by Fragment analysis, in-house and Biomed-2 protocol targets FR1, FR2, FR3 and DJ (van Dongen et al, Leukemia, 2003) BELAC accreditation number: 141-MED Version date: 12/04/2023 Accredited:
CHU Liège - Hemato	IgH clonality analysis	Dr Sc Sabine Franke	Commercial assay: IdentitiClone IgH Gene Clonality Assay, InVivoScribe Technologies. BELAC accreditation number: 128-MED Accredited:
Jessa Hospital	IgH clonality analysis	Dr. Brigitte Maes, Ellen Geerdens	SAMPLE TYPE: -Blood or bone marrow (1ml, EDTA) -Biopsy (fresh or FFPE) -CSF (or other fluids, 1ml) TRANSPORT: room temperature FREQUENCY:1x/week TEST TYPE:PCR & Fragment analysis, in house method from van Dongen et al,Leukemia, 2003, Biomed-2
LHUB-ULB	IgH clonality analysis	Pierre Heimann/Laurent Dewispelaere/Pascale Cochaux/Hakim El Housni	SAMPLE TYPE: Blood or bone marrow (EDTA, 1-2 mL); biopsy; PBSC TRANSPORT: room temperature, within 24h after sampling FREQUENCY: 1/2weeks; max TAT: 5 weeks TEST TYPE: IdentiClone IgH Gene Clonality, Invivoscribe Technologies; IVD-CE labelled kit BELAC accreditation number 650-MED Version date: 04/10/2021 Accredited:
OLVZ Aalst	IgH clonality analysis	Anne Vankeerberghen	Sample type: EDTA-Blood or bone marrow (500 µl) Gland tissue Frequency: 1x in 2 weeks Test type: Fragment analysis (Biomed primers) Version date: 06/05/2019 Accredited:
ZNA Middelheim	IgH clonality analysis	Evelien Heylen	SAMPLE TYPE: -Blood or bone marrow (1ml, EDTA) -Biopsy (fresh or FFPE) -CSF (or other fluids, 1ml) FREQUENCY: 2x/week TEST TYPE: Biomed-2 PCR according to van Dongen et al, Leukemia (2003) Performed in conjunction with IgK and IgL clonality analysis
AZ Delta	IgH clonality analysis	Elisabeth Moreau/Elke Boone	SAMPLE TYPE: -Blood or bone marrow (1ml, EDTA) -Biopsy (fresh or FFPE) -CSF (or other fluids, 1ml) FREQUENCY:1x/week TEST TYPE: PCR & Fragment analysis, Biomed-2 protocol targets FR1, FR2, FR3 and DJ (van Dongen et al,Leukemia, 2003) Version date: 05/12/2017 Accredited:
Labo Cliniques Sud Luxembourg	IgH clonality analysis	Nicolas Hougardy	SAMPLE TYPE: - Blood (EDTA) (min 200µL) - Bone marrow (EDTA) (min 2,5ml) FREQUENCY: 1x/month TEST TYPE: “ Identiclone IGH gene clonality assay”(Invivoscribe), Experion Electrophoresis System (BioRad)
UZ Leuven MolPLUZ	IgH clonality analysis	Jolien De Bie	Sample type: blood / bone marrow (EDTA, 2-5 ml) / tissue (fresh or FFPE) Frequency: 1x/week Test type: PCR on DNA, based on van Dongen et al., Leukemia 2003 (BIOMED-2 protocol). BELAC accreditation number 215-MED Accredited: