BCR-ABL1 p190 (e1a2) quantitative analysis

real time PCR for t(9;22) BCR-ABL p190 (e1a2) fusion gene
Lab Test name Contact Comments
UZA Mol. Diagn. BCR-ABL1 p190 (e1a2) quantitative analysis K. Vermeulen,
M. Le Mercier,
M-B Maes
Quantitative RT-PCR with EAC protocol (Gabert et al, Leukemia 2003; Beillard et al. Leukemia, 2003) with de BCR-ABL mbcr Fusionquant Kit (Ipsogen) on Lightcycler 480
Sample type: blood / bone marrow
BELAC accreditation number 204-MED
Version date: 06/09/2022
IPG Gosselies BCR-ABL1 p190 (e1a2) quantitative analysis P. Vannuffel
C. De Rop
In house method based on Gabert et al, Leukemia, 2003.
Quantitative RT-PCR.
Sample type: Blood and bone marrow.
BELAC accreditation number: 381-MED
Version date: 02/09/2022
UZ Gent BCR-ABL1 p190 (e1a2) quantitative analysis Barbara Denys
Karl Vandepoele
Real-time PCR with EAC primers,
based on Gabert et al, Leukemia 2003;
BELAC accreditation number 087-MED
Version date: 16/03/2021
UZ Brussel - Molecular Hematology BCR-ABL1 p190 (e1a2) quantitative analysis Marleen Bakkus/Barbara Depreter
Sample type: 4 ml EDTA Blood, 2 ml EDTA bone marrow
Transport: room temperature, within 72h after sampling
Frequency: 3x/week
CE-IVD kit: Xpert® BCR-ABL Ultra p190 (Cepheid)
BELAC accreditation number: 141-MED
Version date: 12/04/2023
CHU Liège - Hemato BCR-ABL1 p190 (e1a2) quantitative analysis Dr Frédéric Lambert
As described by Gabert et al., Leukemia, 2003.
Jessa Hospital BCR-ABL1 p190 (e1a2) quantitative analysis Dr. Brigitte Maes, Ellen Geerdens
Blood or bone marrow (1ml, EDTA)
TRANSPORT: room temperature, within 24h after sampling
FREQUENCY: 1x/week
TEST TYPE: Real-time RT-PCR, in house method from Gabert et al
LHUB-ULB BCR-ABL1 p190 (e1a2) quantitative analysis Pierre Heimann/Laurent Dewispelaere/Pascale Cochaux/Hakim El Housni
SAMPLE TYPE: Peripheral blood or bone marrow (EDTA, 1-2 mL)
TRANSPORT: room temperature, within 24h after sampling
FREQUENCY: 1x/week; TAT: 2 weeks
TEST TYPE: Droplet digital PCR
BELAC accreditation number 650-MED
Version date: 04/10/2021
OLVZ Aalst BCR-ABL1 p190 (e1a2) quantitative analysis Anne Vankeerberghen
Sample type:
EDTA-Blood (min. 10 million WBC) or bone marrow (min. 400 µL)
Frequency: 1/week
Test type: Real time PCR (Europe against cancer Program; Beillard et al, 2003)
Version date: 06/05/2019
AZ St-Lucas Gent BCR-ABL1 p190 (e1a2) quantitative analysis Dr H. Louagie / Koen Jacobs
-EDTA blood (4ml)
-EDTA bone marrow

FREQUENCY: 1x/2 weeks

TEST TYPE: Ipsogen FusionQuant
UZ Leuven MolPLUZ BCR-ABL1 p190 (e1a2) quantitative analysis Jolien De Bie
Sample type: blood / bone marrow (EDTA, 2-5 ml)
Frequency: 1x/week
Test type: real time PCR on cDNA (ref: EAC protocol)
BELAC accreditation number 215-MED
AZ Delta BCR-ABL1 p190 (e1a2) quantitative analysis Elisabeth Moreau/Elke Boone
SAMPLE TYPE: Blood or bone marrow (1ml, EDTA)
FREQUENCY: 1x/2-weeks
TEST TYPE: Real-time RT-PCR, EAC protocol (Gabert et al, leukemia 2003)
Version date: 05/12/2017
ZNA Middelheim BCR-ABL1 p190 (e1a2) quantitative analysis Pieter De Schouwer
Sample type: bone marrow or EDTA blood
Frequency: once a week
Test type: in house real-time PCR
Labo Cliniques Sud Luxembourg BCR-ABL1 p190 (e1a2) quantitative analysis Nicolas Hougardy
- Blood (EDTA) (min 10ml)
- Bone Marrow (EDTA) (min 2,5ml)
!The sample has to stay at 4-8°C during transport!
FREQUENCY: every two weeks
TEST TYPE: RT-PCR followed of "BCR-ABL mbcr FusionQuant Kit"(Ipsogen), LightCycler 2.0 (Roche)
ARTICLE: "Gabert,leukemia (2003), 17, 2318-57"