PML-RARA quantitative analysis

real time PCR for t(15;17) PML-RARA fusion genes
Lab Test name Contact Comments
UZ Gent PML-RARA quantitative analysis Barbara Denys,
Karl Vandepoele
Real-time PCR with EAC primers,
based on Gabert et al, Leukemia 2003;
Version date: 11/10/2017
UZA Mol. Diagn. PML-RARA quantitative analysis K. Vermeulen,
M. Le Mercier,
M-B Maes
Quantitative RT-PCR with EAC protocol (Gabert et al, Leukemia 2003; Beillard et al. Leukemia, 2003) for detection of bcr1, bcr2 en bcr3 with de PML-RARA Fusionquant Kit (Ipsogen) on Lightcycler 480
Sample type: blood / bone marrow
Version date: 30/07/2019
UZ Brussel - Molecular Hematology PML-RARA quantitative analysis Marleen Bakkus/Christian Demanet
QPCR for bcr1 and bcr3 (also detects bcr2), primers from EAC consortium, Gabert et al. Leukemia (2003)
Version date: 26/09/2017
CUB Erasme - Medical Genetics Dpt PML-RARA quantitative analysis Pierre Heimann/Barbara Dessars/Hakim El Housni
Real-Time Quantitative PCR
Gabert et al., Leukemia 2003
UZ Leuven MolPLUZ PML-RARA quantitative analysis Lucienne Michaux / Els Lierman
Sample type: blood / bone marrow (EDTA, 2-5ml)
Frequency: 1x/week
Test type: real time PCR on cDNA (EAC protocol)
ZNA Middelheim PML-RARA quantitative analysis Pieter De Schouwer
Sample type: bone marrow or EDTA blood
Frequency: once a week
Test type: in house real-time PCR