PML-RARA quantitative analysis

real time PCR for t(15;17) PML-RARA fusion genes
Lab Test name Contact Comments
UZ Gent PML-RARA quantitative analysis Barbara Denys,
Karl Vandepoele
Real-time PCR with EAC primers,
based on Gabert et al, Leukemia 2003;
BELAC accreditation number 087-MED
Version date: 16/03/2021
UZA Mol. Diagn. PML-RARA quantitative analysis K. Vermeulen,
M. Le Mercier,
M-B Maes
Quantitative RT-PCR with EAC protocol (Gabert et al, Leukemia 2003; Beillard et al. Leukemia, 2003) for detection of bcr1, bcr2 en bcr3 with de PML-RARA Fusionquant Kit (Ipsogen) on Lightcycler 480
Sample type: blood / bone marrow
BELAC accreditation number 204-MED
Version date: 06/09/2022
UZ Brussel - Molecular Hematology PML-RARA quantitative analysis Marleen Bakkus/Barbara Depreter
QPCR for bcr1 and bcr3 (also detects bcr2), primers from EAC consortium, Gabert et al. Leukemia (2003)
BELAC accreditation number: 141-MED
Version date: 12/04/2023
LHUB-ULB PML-RARA quantitative analysis Pierre Heimann/Laurent Dewispelaere/Pascale Cochaux/Hakim El Housni
SAMPLE TYPE: Peripheral blood (5mL) or bone marrow (EDTA, 1-2 mL)
TRANSPORT: room temperature, within 24h after sampling
FREQUENCY: on demand; max TAT: 4 weeks
TEST TYPE: Droplet digital PCR (BCR1 and 3 breakpoints); Laboratory developed test (LDT)
BELAC accreditation number 650-MED
Version date: 04/10/2021
UZ Leuven MolPLUZ PML-RARA quantitative analysis Jolien De Bie
Sample type: blood / bone marrow (EDTA, 2-5ml)
Frequency: 1x/week
Test type: real time PCR on cDNA (EAC protocol)
BELAC accreditation number 215-MED
ZNA Middelheim PML-RARA quantitative analysis Pieter De Schouwer
Sample type: bone marrow or EDTA blood
Frequency: once a week
Test type: in house real-time PCR