BRAF V600E mutation analysis (solid tumor)

Lab Test name Contact Comments
CHU Liège - Hemato BRAF V600E mutation analysis (solid tumor) Dr F. Lambert - Dr Sc. B. Koopmansch
Melanoma, colorectal cancers

SAMPLE TYPE:
FFPE tissues to be sent to the surgical pathology lab with request form.

Pyrosequencing as described by Cynthia Spittle et al. Journal of Molecular Diagnostics, Vol. 9, No. 4, September 2007.
AZ St-Jan Brugge - Pathologie BRAF V600E mutation analysis (solid tumor) Dr. Ivo Van Den Berghe - Dr. Jacques Van Huysse - Ing. Stefanie Vermeire
SAMPLE TYPE: FFPE tissues
FREQUENCY: 1X/week
TEST TYPE: Pyrosequencing
ULB-Erasme Pathology BRAF V600E mutation analysis (solid tumor) Nicky D'Haene
Sample Type: FFPE Tissue
Frequency: On demand
Test Type : Idylla BRAF Mutation Test, Biocartis
Version date: 11/10/2017
Accredited:
AZ Delta BRAF V600E mutation analysis (solid tumor) Geert Martens/Franceska Dedeurwaerdere/Joke Breyne
SAMPLE TYPE: FFPE tissue
FREQUENCY: 1x/week
TEST TYPE: Next Generation Sequencing
Version date: 05/12/2017
Accredited:
UZ Leuven MolPLUZ BRAF V600E mutation analysis (solid tumor) Isabelle Vanden Bempt / Sara Vander Borght
Sample type: FFPE / fresh tissues
Frequency: 2x/ week
Test type: wild type blocking real time PCR on DNA + Sanger sequencing (in house method)
Accredited:
Imelda-Bonheiden BRAF V600E mutation analysis (solid tumor) Dr. Johan Frans, Isabel Micalessi
Sample Type: FFPE
Frequency: Daily
Test Type: BRAF Mutation Test (IDYLLA, Biocartis)
UZ Gent BRAF V600E mutation analysis (solid tumor) Joni Van der Meulen, Nadine Van Roy, Kathleen Claes, Elien De Latter: Moleculaire Diagnostiek UZ Gent (MDG)
Jo Van Dorpe, David Creytens, Isabelle Rottiers: Dienst Pathologische Anatomie UZ Gent
IIn-house developed NGS test:
MDG-colon1 (KRAS, NRAS, BRAF) for metastatic colorectal tumors
MDG-GIST1 (KIT, PDGFRA, BRAF) for gastro-intestinal stromal tumors (GIST) and melanoma
MDG-BRAIN1 (IDH1, IDH2, BRAF, H3F3A) for brain tumors
MDG-LONG1 (EGFR, BRAF, MET, KRAS, ERBB2, ALK, ROS1) for lung tumors
MDG-THYR1 (KRAS, NRAS, HRAS, BRAF) for thyroid tumors

SAMPLE TYPE: FFPE tissue
FREQUENCY: 1X/week
Version date: 18/09/2018
Accredited:
UZ Gent BRAF V600E mutation analysis (solid tumor) Jo Van Dorpe, David Creytens, Isabelle Rottiers: Dienst Pathologische Anatomie UZ Gent
SAMPLE TYPE: FFPE tissue
FREQUENCY: 1X/Week
ASSAY: BRAF mutation analysis with Idylla kit for melanoma
Version date: 18/09/2018
Accredited:
ULB-Erasme Pathology BRAF V600E mutation analysis (solid tumor) Nicky D’Haene
Sample Type: FFPE Tissue
Frequency: 1x/week
Test Type : part of the NGS Cancer Panel (50 genes with BRAF, NRAS, KIT......)
Version date: 11/10/2017
Accredited:
ULB-Erasme Pathology BRAF V600E mutation analysis (solid tumor) Nicky D’Haene
Sample Type: FFPE Tissue
Frequency: 1x/week
Test Type : part of the Colon & Lung NGS panel (22 genes, with KRAS, NRAS, BRAF, ERBB2, PIK3CA........)
Version date: 11/10/2017
Accredited: