EGFR mutation analysis

Lab Test name Contact Comments
Jessa Hospital EGFR mutation analysis Dr. Brigitte Maes, Femke Hillen
SAMPLE TYPE: FFPE tissues
FREQUENCY: 1X/week
ASSAY: Therascreen
UZ Gent EGFR mutation analysis Joni Van der Meulen, Nadine Van Roy, Kathleen Claes, Elien De Latter: Moleculaire Diagnostiek UZ Gent (MDG)
Jo Van Dorpe, David Creytens, Isabelle Rottiers: Dienst Pathologische Anatomie UZ Gent
In-house developed NGS test:
MDG-LONG1 (EGFR, BRAF, MET, KRAS, ERBB2, ALK, ROS1) for lung tumors

SAMPLE TYPE: FFPE tissue
FREQUENCY: 1X/week
Version date: 18/09/2018
Accredited:
AZ St-Lucas Gent EGFR mutation analysis Dr. H. Louagie/ Koen Jacobs
SAMPLE TYPE: FFPE biopsy

FREQUENCY: 1x/2 weeks

TEST TYPE: Therascreen
CHU Liège - Hemato EGFR mutation analysis Dr F. Lambert - Dr Sc. B. Koopmansch
SAMPLE TYPE:
FFPE tissues to be sent to the surgical pathology lab with request form.

EGFR exon 19: fragment analysis.
EGFR exons 21: allelic discrimination (exon 20 upon request if ongoing TKI treatment).

As described by Rosell et al., NEJM 2009; Taron et al. Clin Cancer Res 2005; Molina-Vila J Thoracic Oncol 2009.

TAT: 5-10 days.
AZ Delta EGFR mutation analysis Geert Martens/Franceska Dedeurwaerdere/Joke Breyne
SAMPLE TYPE: FFPE tissue
FREQUENCY: 1x/week
TEST TYPE: Next Generation Sequencing
Version date: 05/12/2017
Accredited:
AZ St-Jan Brugge - Pathologie EGFR mutation analysis Dr. Ivo Van Den Berghe - Dr. Jacques Van Huysse - Ing. Stefanie Vermeire
SAMPLE TYPE: FFPE tissues
FREQUENCY: 1X/week
TEST TYPE: Pyrosequencing
UZ Gent EGFR mutation analysis Jo Van Dorpe, David Creytens, Isabelle Rottiers: Dienst Pathologische Anatomie UZ Gent
SAMPLE TYPE: FFPE tissue
FREQUENCY: 1X/Week
ASSAY: EGFR mutation analysis with Idylla kit for lung tumors
Version date: 18/09/2018
Accredited:
ULB-Erasme Pathology EGFR mutation analysis Nicky D’Haene
Sample Type: FFPE Tissue
Frequency: 1x/week
Test Type : part of the Colon & Lung NGS panel (22 genes, with EGFR, KRAS, BRAF, ERBB2, MET, ALK........)
Version date: 11/10/2017
Accredited:
ULB-Erasme Pathology EGFR mutation analysis Nicky D’Haene
Ricardo De Mendonça
Sample Type: Plasma (Blood samples in Streck Tubes)
Frequency: 1x/week
Test Type : digital droplet PCR (ddPCR) with PrimePCR ddPCR Mutation Assay kit (Biorad) for p.L858R (exon 21), p.E746_A750del (exon 19) et p.T790M (exon 20) mutations
Version date: 20/06/2017
Accredited:
UZ Leuven MolPLUZ EGFR mutation analysis Isabelle Vanden Bempt / Sara Vander Borght
Sample Type: Plasma (Blood samples in STRECK tubes or equivalent like Cell-free DNA Collection Tube, Roche)
Frequency: 1x/week
Test Type : digital droplet PCR (ddPCR) with PrimePCR ddPCR Mutation Assay (Bio-Rad) for p.L858R (exon 21), deletions exon 19 and p.T790M (exon 20) mutations
Accredited:
ULB-Erasme Pathology EGFR mutation analysis Nicky D'Haene
Sample Type: FFPE Tissue
Frequency: On demand
Test Type : Idylla EGFR Mutation Test, Biocartis
Version date: 08/09/2017
Accredited:
AZ Delta EGFR mutation analysis Geert Martens/Joke Breyne
SAMPLE TYPE: Streck plasma (liquid biopsy)
FREQUENCY: upon request
TEST TYPE: Cobas EGFR mutation test v2
Version date: 05/12/2017
Accredited: