KIT/PDGFRA mutation analysis (GIST)

Lab	Test name	Contact	Comments
CHU Liège - Hemato	KIT/PDGFRA mutation analysis (GIST)	Dr F. Lambert - Dr Sc. B. Koopmansch	In case of suspected GISTs. SAMPLE TYPE: FFPE tissues to be sent to the surgical pathology lab with request form. PCR plus fragment analysis followed by direct sequencing of c-KIT exons 9 and 11 or PDGFRA exon 12 in case of abnormal traces.
UZ Leuven MolPLUZ	KIT/PDGFRA mutation analysis (GIST)	Isabelle Vanden Bempt / Sara Vander Borght	Sample type: FFPE / fresh tissues Frequency: 2x/ week Test type: PCR on DNA + Sanger sequencing of exons 9, 11, 13 and 17 of KIT (exon 14 also possible), and exons 12, 14 and 18 of PDGFRA (in house method) Accredited:
UZ Gent	KIT/PDGFRA mutation analysis (GIST)	Joni Van der Meulen: Moleculaire Diagnostiek UZ Gent (MDG) Jo Van Dorpe, David Creytens, Isabelle Rottiers: Dienst Pathologische Anatomie UZ Gent	In-house developed NGS test: MDG-GIST1 (KIT, PDGFRA and BRAF) SAMPLE TYPE: FFPE tissue FREQUENCY: 1X/Week ASSAY: MDG-GIST1 test, NGS Version date: 28/09/2017 Accredited:
ULB-Erasme Pathology	KIT/PDGFRA mutation analysis (GIST)	Nicky D’Haene	Sample Type: FFPE Tissue Frequency: 1x/week Test Type : part of the NGS Cancer Panel (50 genes with KIT, PDGFRA, BRAF......) Version date: 11/10/2017 Accredited: