hematology - lymfoid gene panels

Lab Test name Contact Comments
UZA Mol. Diagn. hematology - lymfoid gene panels K. Vermeulen,
M. Le Mercier,
M-B Maes
57 genes Haloplex HS NGS panel on MiSeq to screen clinically relevant genes in lymphoid malignancies (incl. CLL, DLBCL, LP, etc).
Genes: ATM, B2M, BCL2, BIRC3, BRAF, BTK, CARD11, CD28, CD58, CD79A, CD79B, CDKN2A, CREBBP, CXCR4, DNMT3A, DDX3X, EP300, EZH2, FBXW7, FGFR1, FGFR2, FGFR3, FOXO1, GNA13, ID3, IDH1, IDH2, IKZF1, IRF4, JAK1, JAK2, JAK3, KMT2D/MLL2, KRAS, MAP2K1, MEF2B, MYC, MYD88, NFKBIE, NOTCH1, NOTCH2, NRAS, PDGFRA, PIM1, PLCG2, RHOA, SF3B1, SOCS1, STAT3, STAT5B, STAT6, TCF3, TET2, TNFAIP3, TNFRSF14, TP53, XPO1.
Sample Type: Blood, Bone marrow, Biopt, FFPE samples
Version date: 25/01/2021
Accredited:
CHU Liège - Hemato hematology - lymfoid gene panels Dr Frédéric LAMBERT, Benjamin KOOPMANSCH & Rafael Carazo
Inherited Syndromes Predisposing to Adult-Onset Hematologic Malignancy:
Familial B-cell ALL with PAX5; Germline SH2B3; Li-Fraumeni syndrome with ALL or therapy-related AML (TP53)

NGS targeted sequencing after hybridization capture, Home brew assay.

Skin biopsy requested or fibroblasts'DNA.

INFORMED CONSENT REQUESTED BEFORE TESTING:
http://www.chu.ulg.ac.be/jcms/c_10919499/formulaire-de-consentement-ngs

BELAC accreditation number: 128-MED
Accredited:
UZ Leuven MolPLUZ hematology - lymfoid gene panels Jolien De Bie
Sample type: blood / bone marrow (EDTA, 2-5 ml)
Frequency: 1x/week
Test type: targeted capture NGS workflow with custom IDT xGen® Lockdown® Probes on an Illumina sequencing instrument
Genes: ACTG1, ANKRD26, ATM, BCOR, BCORL1, BIRC3, BRAF, BTK, CARD11, CREBBP, CRLF2, CXCR4, CYLD, DDX41, DIS3, DNMT3A, EBF1, EGR1, ELANE, ETV6, EZH2, FAM46C, FBXW7, FGFR3, GATA2, GFI1, HRAS, IKZF1, IL7R, IRF4, JAK1, JAK3, KRAS, MYD88, NOTCH1, NRAS, PHF6, PLCG2, POT1, PPM1D, PRDM1, PRKD2, PTEN, PTPN11, RAD21, RB1, SAMD9, SAMD9L, SETD2, SF3B1, SMC1A, SMC3, SP140, SRP72, STAG1, STAG2, STAT5B, TP53, TRAF3, WAS, XPO1
BELAC accreditation number 215-MED
Accredited:
Jessa Hospital hematology - lymfoid gene panels Dr. Brigitte Maes, Guy Froyen
AmpliSeq myeloid panel (Illumina) (DNA + RNA)
Genes: ABL1, ASXL1, ATRX, BCOR, BCORL1, BRAF, CALR, CBL, CBLB, CBLC, CDKN2A, CEBPA, CSF3R, CUX1, DNMT3A, ETV6, EZH2, FBXW7, FLT3, GATA1, GATA2, GNAS, HRAS, IDH1, IDH2, IKZF1, JAK2, JAK3, KDM6A, KIT, KRAS, KMT2A, MPL, MYD88, NOTCH1, NPM1, NRAS, PDGFRA, PHF6, PTEN, PTPN11, RAD21, RUNX1, SETBP1, SF3B1, SMC1A, SMC3, SRSF2, STAG2, TET2, TP53, U2AF1, WT1, ZRSR2
2x/week
on MiSeq
Accredited 275-MED
Version date: 03/08/2021
Accredited:
AZ Delta hematology - lymfoid gene panels Geert Martens, Elke Boone
Sample type: blood / bone marrow (300µl - 1 ml)
Frequency: 1x/month
Test type: TruSight Myeloid (Illumina), NGS
Genes: BRAF, FBXW7, IKZF1, MYD88, NOTCH1, PTEN, SF3B1, TP53, ...
Version date: 05/12/2017
Accredited:
UZ Gent hematology - lymfoid gene panels Karl Vandepoele,
Joni Van Der Meulen,
Barbara Denys
In-house developed, capture-based (HyperCap) 68 gene panel to screen clinically relevant genes in hematological disorders by NGS:
ANKRD26, ASXL1, ATM, BCL2, BCOR, BCORL1, BRAF, BTK, CALR, CBL, CBLB, CEBPA, CSF3R, CUX1, CXCR4, DDX41, DNMT3A, ETNK1, ETV6, EZH2, FBXW7, FLT3, GATA1, GATA2, GNAS, HRAS, IDH1, IDH2, JAK2, JAK3, KIT, KMT2A, KRAS, MPL, MYD88, NF1, NPM1, NOTCH1, NRAS, NUDT15, PHF6, PLCG2, PPM1D, PTPN11, RAD21, RHOA, RRAS, RUNX1, SBDS, SETBP1, SF1, SF3B1, SH2B3, SMC1A, SMC3, SRSF2, STAG2, STAT3, STAT5B, TET2, TP53, TPMT, U2AF1, UGT1A1, WT1, ZRSR2
1x/week
BELAC accreditation number 087-MED
Version date: 16/03/2021
Accredited:
UZ Brussel - Molecular Pathology hematology - lymfoid gene panels Freya Vaeyens
NGS targeted sequencing after hybridization capture, home brew STHT assay (solid and hematological tumors)
Genes list available at http://www.brightcore.be/stht
Sample Type: blood/ bone marrow
Frequency: 1x/week
Accreditation: BELAC 141-MED
Version date: 10/10/2019
Accredited:
UZ Brussel - Molecular Pathology hematology - lymfoid gene panels Freya Vaeyens
Fusion NGS panel - TSO500 Illumina
Sample Type: blood / bone marrow
Frequency: 1x/week
Version date: 10/02/2021
LHUB-ULB hematology - lymfoid gene panels Pierre Heimann/Laurent Dewispelaere/Pascale Cochaux/Hakim El Housni
TRANSPORT: room temperature, within 24h after sampling
FREQUENCY: 1x/week; max TAT: 4 weeks
TEST TYPE: In-house developed gene panel using the Qiaseq Targeted DNA custom Panels platform (Qiagen) to screen for variants in lymphoid diseases:
BRAF, BTK, CXCR4, KRAS, MYD88, NRAS, SF3B1, TP53

BELAC accreditation number 650-MED
Version date: 04/10/2021
Accredited:
LHUB-ULB hematology - lymfoid gene panels Pierre Heimann/Laurent Dewispelaere/Pascale Cochaux/Hakim El Housni
TRANSPORT: room temperature, within 24h after sampling
FREQUENCY: 1x/week; max TAT: 4 weeks
TEST TYPE: In-house developed gene panel using the Qiaseq Targeted DNA custom Panels platform (Qiagen) to screen for variants in acute T-cell leukemia:
FBXW7, HRAS, KRAS, NOTCH1, NRAS, PTEN, TP53

BELAC accreditation number 650-MED
Version date: 04/10/2021