hematology - lymfoid gene panels

Lab Test name Contact Comments
UZA Mol. Diagn. hematology - lymfoid gene panels K. Vermeulen,
M. Le Mercier,
M-B Maes
57 genes Haloplex HS NGS panel on MiSeq to screen clinically relevant genes in lymphoid malignancies (incl. CLL, DLBCL, LP, etc).
Genes: ATM, B2M, BCL2, BIRC3, BRAF, BTK, CARD11, CD28, CD58, CD79A, CD79B, CDKN2A, CREBBP, CXCR4, DNMT3A, DDX3X, EP300, EZH2, FBXW7, FGFR1, FGFR2, FGFR3, FOXO1, GNA13, ID3, IDH1, IDH2, IKZF1, IRF4, JAK1, JAK2, JAK3, KMT2D/MLL2, KRAS, MAP2K1, MEF2B, MYC, MYD88, NFKBIE, NOTCH1, NOTCH2, NRAS, PDGFRA, PIM1, PLCG2, RHOA, SF3B1, SOCS1, STAT3, STAT5B, STAT6, TCF3, TET2, TNFAIP3, TNFRSF14, TP53, XPO1.
Sample Type: Blood, Bone marrow, Biopt
Version date: 30/07/2019
Accredited:
CHU Liège - Hemato hematology - lymfoid gene panels Dr Frédéric LAMBERT & Benjamin KOOPMANSCH
Inherited Syndromes Predisposing to Adult-Onset Hematologic Malignancy:
Familial B-cell ALL with PAX5; Germline SH2B3; Li-Fraumeni syndrome with ALL or therapy-related AML (TP53)

NGS targeted sequencing after hybridization capture, Home brew assay; ISO15189 accreditation from BELAC.

Skin biopsy requested or fibroblasts'DNA.

INFORMED CONSENT REQUESTED BEFORE TESTING:
http://www.chu.ulg.ac.be/jcms/c_10919499/formulaire-de-consentement-ngs
UZ Leuven MolPLUZ hematology - lymfoid gene panels Lucienne Michaux / Els Lierman
Sample type: blood / bone marrow (EDTA, 2-5 ml)
Frequency: 1x/week
Test type: TruSight Myeloid (Illumina), NGS
Genes: BRAF, FBXW7, IKZF1, MYD88, NOTCH1, PTEN, SF3B1, TP53, ...
Accredited:
UZ Leuven MolPLUZ hematology - lymfoid gene panels Lucienne Michaux / Els Lierman
Sample type: blood / bone marrow (EDTA, 2-5 ml)
Frequency: 1x/week
Test type: MLPA for detection of micro-deletions and -duplications in B-ALL (SALSA MLPA probemix P335-B1 ALL-IKZF1)
Genes: EBF1, IKZF1, CDKN2A, CDKN2B, PAX5, ETV6, BTG1, RB1, SHOX, CRLF2, CSF2RA, IL3RA, P2RY8
Accredited:
Jessa Hospital hematology - lymfoid gene panels Guy Froyen
2x/week
on MiSeq
Version date: 13/11/2017
Accredited:
AZ Delta hematology - lymfoid gene panels Geert Martens, Elke Boone
Sample type: blood / bone marrow (300µl - 1 ml)
Frequency: 1x/month
Test type: TruSight Myeloid (Illumina), NGS
Genes: BRAF, FBXW7, IKZF1, MYD88, NOTCH1, PTEN, SF3B1, TP53, ...
Version date: 05/12/2017
Accredited:
UZ Gent hematology - lymfoid gene panels Karl Vandepoele,
Joni Van Der Meulen,
Barbara Denys
In-house developed, capture-based (SeqCap) 64 gene panel to screen clinically relevant genes in hematological disorders by NGS:
ANKRD26, ASXL1, ATM, BCL2, BCOR, BCORL1, BIRC3, BRAF, BTK, CALR, CBL, CEBPA, CRLF2, CSF3R, CUX1, DDX41, DNMT3A, EGR2, ETNK1, ETV6, EZH2, FBXW7, FLT3, GATA2, HRAS, IDH1, IDH2, IKZF1, IL7R, JAK2, JAK3, KIT, KRAS, MPL, NF1, NFKBIE, NPM1, NOTCH1, NRAS, PAX5, PHF6, PLCG2, POT1, PPM1D, PTPN11, RAD21, RPS15, RRAS, RUNX1, SETBP1, SF1, SF3B1, SH2B3, SMC1A, SMC3, SRSF2, STAG2, STAT5B, TET2, TP53, U2AF1, WT1, XPO1, ZRSR2
1x/week
Version date: 26/12/2019
Accredited: