hematology - translocation screening

Lab Test name Contact Comments
AZ St-Jan Brugge - Laboratoriumgeneeskunde hematology - translocation screening dr. Helena Devos/Friedel Nollet, Ph.D.
Multiplex, real time PCR HemaVision kit for the screening and detection of 28 chromosomal translocations; RNA-test
Version date: 26/09/2017
UZ Gent hematology - translocation screening Barbara Denys,
Karl Vandepoele
Technology: HemaVision kit (DNA Diagnostic) for the screening and detection of 28 chromosome translocations with more than 145 clinically relevant chromosomal breakpoints
Version date: 18/05/2017
UZ Brussel - Molecular Hematology hematology - translocation screening Marleen Bakkus/Christian Demanet
Hemavision screenings panel for 28 different translocations: t(1;19):E2A/PBX1, t(3;5):NPM/MLF1, t(3;21):AML1/MDS1, t(5;12):TEL/PDGFRb, t(5;17):NPM/RARA, t(6;9):DEK/CAN, t(8;21):AML1/MGT8, t(9;9):SET/CAN, t(9;12):TEL/ABL, t(9;22):BCR/ABL, t(11;17)PLZF/RARA, t(12;21):TEL/AML1, t(12;22):TEL/MN1, t(15;17):PML/RARA, t(16;21):TLS/ERG, inv16:CBFb/MYH11, t(17;19):E2A/HLF, TAL1D:SIL1/TAL1 and the MLL1 with several fusion partners t(1;11):MLL1/AF1p, t(1;11):MLL1/AF1q, t(4;11): MLL1/AF4, t(6;11):MLL1/AF6, t(9;11):MLL1/AF9, t(10;11):MLL1/AF10, t(11;17):MLL1/AF17, t(11;19):MLL1/ELL, t(11;19):MLL1/ENL, and t(X;11):MLL1/AFX1.
Version date: 26/09/2017
CHU Li├Ęge - Hemato hematology - translocation screening Dr Sc Sabine Franke
DNA Diagnostic - HemaVision - 28Q -
Screening for BCR-ABL1 (p190, p210, p230) ; TEL-RUNX1 (AML1); E2A-PBX1; MLL(KMT2A)-AF4; SIL-TAL1; AML1-ETO; PML-RARA; MYH11-CBCB; MLL(KMT2A)-AF6; MLL-AF9(KMT2A); MLL(KMT2A)-AFX;
Full details: DNA Diagnostic; http://dna-diagnostic.com/products/hemavision/hemavision-28q/
Jessa Hospital hematology - translocation screening Dr. Brigitte Maes, Femke Hillen
Blood or bone marrow (1ml, EDTA)
TRANSPORT: room temperature, within 24h after sampling
FREQUENCY: 1x/week
TEST TYPE: RT-PCR with Hemavision-7 kit (detection of AML1-ETO, CBFb-MYH, PML-RARalfa, BCR-ABL1, E2A-PBX1, TEL-AML1, MLL-AF4)
ZNA Middelheim hematology - translocation screening Pieter De Schouwer
Sample type: bone marrow or EDTA blood
Frequency: once a week
Test type: commercial kit (HemaVision Multiplex PCR kit)
UZA Mol. Diagn. hematology - translocation screening K. Vermeulen, M-B Maes
AZ Delta hematology - translocation screening Geert martens, Elke Boone
SAMPLE TYPE: blood or bone marrow (1ml)
FREQUENCY: upon request
ANALYSIS TYPE: RNA test, Hemavision 28Q real-time PCR on cDNA
Detected fusion transcripts: BCR-ABL1/t(9;22)(q34;q11); CBFB-MYH11/inv(16)(p13;q22); DEK-NUP214/t(6;9)(p23;q34); ETV6-RUNX1/t(12;21)(p13;q22); KMT2A-AFF1/t(4;11)(q21;q23); KMT2A-MLLT1/ t(11;19)(q23;p13.3); PML-RARA/t(15;17)(q24;q21); RUNX1-RUNX1T1/t(8;21)(q22;q22); STIL-TAL1/del(1)(p32) en TCF3-PBX1/ t(1;19)(q23;p13); ETV6-ABL1/ t(9;12)(q34;p13); ETV6-MN1/t(12;22)(p13;q11-12); ETV6-PDGFRB/ t(5;12)(q33;p13); FUS-ERG/t(16;21)(p11;q22); KMT2A-ELL/t(11;19)(q23;p13.1); KMT2A-EPS15/t(1;11)(p32;q23); KMT2A-FOXO4/t(X;11)(q13;q23); KMT2A-MLLT3/ t(9;11)(p22;q23); KMT2A-MLLT4/t(6;11)(q27;q23); KMT2A-MLLT6/t(11;17)(q23;q21); KMT2A-MLLT10/t(10;11)(p12;q23); KMT2A-MLLT11/t(1;11)(q21;q23); NPM1-MLF1/t(3;5)(q25.1;q35); NPM1-RARA/t(5;17)(q35;q12); RUNX1-MECOM/ t(3;21)(q26;q22); SET-NUP214/t(9;9)(q34;q34); TCF3-HLF/t(17;19)(q22;p13)en ZBTB16-RARA/t(11;17)(q23;q21).
Version date: 05/12/2017
UZ Gent hematology - translocation screening Barbara Denys,
Karl Vandepoele
Multiplex, in-house developed, qPCR assay for the screening and detection of 4 chromosomal translocations in pediatric, adolescents and young adults (< 40 year old) with AML that do not have another translocation.

NUP98-NSD1 of t(5;11)(q35;p15.5)
NUP98-KDM5A of t(11;12)(p15;p13)
CBFA2T3-GLIS2 of inv(16)(p13q24)
OTT-MAL/RBM15-MKL1 of t(1;22)(p13;q13)

When a translocation has been identified, the singleplex qPCR reactions can be used to determine minimal/measurable detectable disease (see hematology - translocations for these tests)
Version date: 15/01/2019