hematology - translocation screening

Lab Test name Contact Comments
AZ St-Jan Brugge - Laboratoriumgeneeskunde hematology - translocation screening dr. Helena Devos/Friedel Nollet, Ph.D.
Multiplex, real time PCR HemaVision kit for the screening and detection of 28 chromosomal translocations; RNA-test
Version date: 26/09/2017
Accredited:
UZ Gent hematology - translocation screening Barbara Denys,
Karl Vandepoele
Technology: HemaVision qPCR kit (DNA Diagnostic) for the screening and detection of 28 chromosome translocations with more than 145 clinically relevant chromosomal breakpoints
BELAC accreditation number 087-MED
Version date: 16/03/2021
Accredited:
UZ Brussel - Molecular Hematology hematology - translocation screening Marleen Bakkus/Christian Demanet
Hemavision screenings panel for 28 different translocations: t(1;19):E2A/PBX1, t(3;5):NPM/MLF1, t(3;21):AML1/MDS1, t(5;12):TEL/PDGFRb, t(5;17):NPM/RARA, t(6;9):DEK/CAN, t(8;21):AML1/MGT8, t(9;9):SET/CAN, t(9;12):TEL/ABL, t(9;22):BCR/ABL, t(11;17)PLZF/RARA, t(12;21):TEL/AML1, t(12;22):TEL/MN1, t(15;17):PML/RARA, t(16;21):TLS/ERG, inv16:CBFb/MYH11, t(17;19):E2A/HLF, TAL1D:SIL1/TAL1 and the MLL1 with several fusion partners t(1;11):MLL1/AF1p, t(1;11):MLL1/AF1q, t(4;11): MLL1/AF4, t(6;11):MLL1/AF6, t(9;11):MLL1/AF9, t(10;11):MLL1/AF10, t(11;17):MLL1/AF17, t(11;19):MLL1/ELL, t(11;19):MLL1/ENL, and t(X;11):MLL1/AFX1.
Version date: 26/09/2017
Accredited:
CHU Liège - Hemato hematology - translocation screening Dr Sc Sabine Franke
DNA Diagnostic - HemaVision - 28Q -
Screening for BCR-ABL1 (p190, p210, p230) ; TEL-RUNX1 (AML1); E2A-PBX1; MLL(KMT2A)-AF4; SIL-TAL1; AML1-ETO; PML-RARA; MYH11-CBCB; MLL(KMT2A)-AF6; MLL-AF9(KMT2A); MLL(KMT2A)-AFX;
Full details: DNA Diagnostic; http://dna-diagnostic.com/products/hemavision/hemavision-28q/

BELAC accreditation number: 128-MED
Accredited:
Jessa Hospital hematology - translocation screening Dr. Brigitte Maes, Ellen Geerdens
SAMPLE TYPE:
Blood or bone marrow (1ml, EDTA)
TRANSPORT: room temperature, within 24h after sampling
FREQUENCY: 1x/week
TEST TYPE: RT-PCR with Hemavision-7 kit (detection of AML1-ETO, CBFb-MYH, PML-RARalfa, BCR-ABL1, E2A-PBX1, TEL-AML1, MLL-AF4)
ZNA Middelheim hematology - translocation screening Pieter De Schouwer
Sample type: bone marrow or EDTA blood
Frequency: once a week
Test type: commercial kit (HemaVision Multiplex PCR kit)
UZA Mol. Diagn. hematology - translocation screening K. Vermeulen,
M. Le Mercier,
M-B Maes
Multiplex, real-time RT-PCR on RNA. Detection of 28 fusion genes. Kit Hemavision 28Q.
Detected fusion transcripts: BCR-ABL1/t(9;22)(q34;q11); CBFB-MYH11/inv(16)(p13;q22); DEK-NUP214/t(6;9)(p23;q34); ETV6-RUNX1/t(12;21)(p13;q22); KMT2A-AFF1/t(4;11)(q21;q23); KMT2A-MLLT1/ t(11;19)(q23;p13.3); PML-RARA/t(15;17)(q24;q21); RUNX1-RUNX1T1/t(8;21)(q22;q22); STIL-TAL1/del(1)(p32) en TCF3-PBX1/ t(1;19)(q23;p13); ETV6-ABL1/ t(9;12)(q34;p13); ETV6-MN1/t(12;22)(p13;q11-12); ETV6-PDGFRB/ t(5;12)(q33;p13); FUS-ERG/t(16;21)(p11;q22); KMT2A-ELL/t(11;19)(q23;p13.1); KMT2A-EPS15/t(1;11)(p32;q23); KMT2A-FOXO4/t(X;11)(q13;q23); KMT2A-MLLT3/ t(9;11)(p22;q23); KMT2A-MLLT4/t(6;11)(q27;q23); KMT2A-MLLT6/t(11;17)(q23;q21); KMT2A-MLLT10/t(10;11)(p12;q23); KMT2A-MLLT11/t(1;11)(q21;q23); NPM1-MLF1/t(3;5)(q25.1;q35); NPM1-RARA/t(5;17)(q35;q12); RUNX1-MECOM/ t(3;21)(q26;q22); SET-NUP214/t(9;9)(q34;q34); TCF3-HLF/t(17;19)(q22;p13)en ZBTB16-RARA/t(11;17)(q23;q21).
Version date: 30/07/2019
Accredited:
AZ Delta hematology - translocation screening Geert martens, Elke Boone
SAMPLE TYPE: blood or bone marrow (1ml)
FREQUENCY: upon request
ANALYSIS TYPE: RNA test, Hemavision 28Q real-time PCR on cDNA
Detected fusion transcripts: BCR-ABL1/t(9;22)(q34;q11); CBFB-MYH11/inv(16)(p13;q22); DEK-NUP214/t(6;9)(p23;q34); ETV6-RUNX1/t(12;21)(p13;q22); KMT2A-AFF1/t(4;11)(q21;q23); KMT2A-MLLT1/ t(11;19)(q23;p13.3); PML-RARA/t(15;17)(q24;q21); RUNX1-RUNX1T1/t(8;21)(q22;q22); STIL-TAL1/del(1)(p32) en TCF3-PBX1/ t(1;19)(q23;p13); ETV6-ABL1/ t(9;12)(q34;p13); ETV6-MN1/t(12;22)(p13;q11-12); ETV6-PDGFRB/ t(5;12)(q33;p13); FUS-ERG/t(16;21)(p11;q22); KMT2A-ELL/t(11;19)(q23;p13.1); KMT2A-EPS15/t(1;11)(p32;q23); KMT2A-FOXO4/t(X;11)(q13;q23); KMT2A-MLLT3/ t(9;11)(p22;q23); KMT2A-MLLT4/t(6;11)(q27;q23); KMT2A-MLLT6/t(11;17)(q23;q21); KMT2A-MLLT10/t(10;11)(p12;q23); KMT2A-MLLT11/t(1;11)(q21;q23); NPM1-MLF1/t(3;5)(q25.1;q35); NPM1-RARA/t(5;17)(q35;q12); RUNX1-MECOM/ t(3;21)(q26;q22); SET-NUP214/t(9;9)(q34;q34); TCF3-HLF/t(17;19)(q22;p13)en ZBTB16-RARA/t(11;17)(q23;q21).
Version date: 05/12/2017
Accredited:
UZ Gent hematology - translocation screening Karl Vandepoele,
Joni Van der Meulen,
Nadine Van Roy,
Barbara Denys
Archer® FusionPlex ALL panel to screen for fusion transcripts involving ABL1, ABL2, BCL11B, BCL2, BCL6, BCR, CHD1, CREBBP, CRLF2, CSF1R, EBF1, EPOR, ETV6, FGFR1, IKZF1, IKZF2, IKZF3, JAK2, KLF2, KMT2A, MLLT4, MYC, NF1, NOTCH1, NTRK3, NUP214, NUP98, P2RY8, PAG1, PAX5, PBX1, PDCD1LG2, PDGFRA, PDGFRB, PICALM, PTK2B, RUNX1, SEMA6A, SETD2, STIL, TAL1, TCF3, TYK2, ZCCHC7.
1x/week
BELAC accreditation number 087-MED
Version date: 16/03/2021
Accredited:
UZ Gent hematology - translocation screening Barbara Denys,
Karl Vandepoele
Multiplex, in-house developed, qPCR assay for the screening and detection of 4 chromosomal translocations in pediatric, adolescents and young adults (< 40 year old) with AML that do not have another translocation.

NUP98-NSD1 or t(5;11)(q35;p15.5)
NUP98-KDM5A or t(11;12)(p15;p13)
CBFA2T3-GLIS2 or inv(16)(p13q24)
OTT-MAL/RBM15-MKL1 or t(1;22)(p13;q13)

When a translocation has been identified, the singleplex qPCR reactions can be used to determine minimal/measurable detectable disease (see hematology - translocations for these tests)
Version date: 18/04/2019
Accredited:
UZA Mol. Diagn. hematology - translocation screening K. Vermeulen,
M. Le Mercier,
M-B Maes
Archer® FusionPlex Heme v2 NGS panel to screen for fusion transcripts involving 72 clinically relevant genes in hematological disorders: ABL1, ABL2, ALK, BCL11B, BCL2, BCL6, BCR, BIRC3, CBFB, CCND1, CCND3, CDK6, CHD1, CHIC2, CIITA, CREBBP, CRLF2, CSF1R, DEK, DUSP22, EBF1, EIF4A1, EPOR, ERG, ETV6, FGFR1, GLIS2, IKZF1, IKZF2, IKZF3, JAK2, KAT6A, KLF2, KMT2A, MALT1, MECOM, MKL1, MLF1, MLLT10, MLLT4, MYC, MYH11, NF1, NFKB2, NOTCH1, NTRK3, NUP214, NUP98, P2RY8, PAG1, PAX5, PDCD1LG2, PDGFRA, PDGFRB, PICALM, PML, PRDM16, PTK2B, RARA, RBM15, ROS1, RUNX1, RUNX1T1, SEMA6A, SETD2, STIL, TAL1, TCF3, TFG, TP63, TYK2, ZCCHC7.
Sample Type: Blood, Bone marrow.
Version date: 25/01/2021
Accredited: