solid tumors - gene panels

Lab Test name Contact Comments
AZ Delta solid tumors - gene panels Geert Martens/Franceska Dedeurwaerdere/Joke Breyne
In-house NGS panel (SeqCap EZ Choice custom design, KAPA Hyperplus workflow on Miseq) for the detection of mutations, indels, CNV in the following genes:
Lung panel (ALK,BRAF, CDKN2A, DDR2,EGFR,ERBB2,KRAS,MET,PIK3CA,RET,ROS1,TP53)
Colon panel (BRAF,KRAS,NRAS,PIK3CA,TP53)
Melanoma panel (BRAF,KIT,NRAS)
GIST panel (BRAF,KIT,PDGFRA)
BRAIN panel (BRAF,H3F3A,IDH1/2)
BREAST/OVARIAN panel (BRCA1/2,ERBB2,ESR1,PIK3CA,TP53)
Other genes tested but not standard reported: AKT1,APC,ARAF,CTNNB1,ERBB4,GNAS,HRAS,MAP2K1,NF1,NRG1,NTRK1,PIK3R1,PTEN,SMAD4,TERT
Version date: 05/12/2017
Accredited:
UZ Gent solid tumors - gene panels Joni Van der Meulen, Nadine Van Roy, Kathleen Claes, Elien De Latter: Moleculaire Diagnostiek UZ Gent (MDG)
Jo Van Dorpe, David Creytens, Isabelle Rottiers: Dienst Pathologische Anatomie UZ Gent
In-house developed NGS test:
MDG-colon1 (KRAS, NRAS, BRAF) for metastatic colorectal tumors
MDG-GIST1 (KIT, PDGFRA, BRAF) for gastro-intestinal stromal tumors (GIST) and melanoma
MDG-BRAIN1 (IDH1, IDH2, BRAF, H3F3A) for brain tumors
MDG-LONG1 (EGFR, BRAF, MET, KRAS, ERBB2, ALK, ROS1) for lung tumors
MDG-PANC1 (KRAS, GNAS) for mucinous neoplasm of the pancreas
MDG-THYR1 (KRAS, NRAS, HRAS, BRAF) for thyroid tumors
MDG-BORST1 (ESR1, PIK3CA) for ER+ HER2- metastatic breast tumors

SAMPLE TYPE: FFPE tissue
FREQUENCY: 1X/week
Version date: 18/09/2018
Accredited:
UZ Gent solid tumors - gene panels Joni Van der Meulen, Nadine Van Roy, Kathleen Claes, Elien De Latter: Moleculaire Diagnostiek UZ Gent (MDG)
Jo Van Dorpe, David Creytens, Isabelle Rottiers: Dienst Pathologische Anatomie UZ Gent
In-house developed NGS test:
MDG-ENDO1 mini (CTNNB1, POLE) for endometrial tumors and hepatocellular carcinoma
MDG-ENDO1 (CTNNB1, POLE, TP53) for endometrial tumors
PRECIS2 (EGFR, ERBB2, ERBB3) for advanced tumors

SAMPLE TYPE: FFPE tissue
FREQUENCY: 1X/week
Version date: 18/09/2018
CHU Liège - Hemato solid tumors - gene panels Dr Frédéric LAMBERT & Benjamin Koopmansch
Amplicon sequencing.
Genes list available at: http://www.chu.ulg.ac.be/jcms/c_9635594/informations-pratiques-relatives-aux-analyses
UZ Leuven MolPLUZ solid tumors - gene panels Isabelle Vanden Bempt / Sara Vander Borght
Sample type: FFPE / fresh tissues
Frequency: 2x/ week
Test type: TruSight Tumor26 (Illumina), NGS
Genes: AKT1, ALK, APC, BRAF, CDH1, CTNNB1, EGFR, ERBB2, FBXW7, FGFR2, FOXL2, GNAQ, GNAS, KIT, KRAS, MAP2K1, MET, MSH6, NRAS, PDGFRA, PIK3CA, PTEN, SMAD4, SRC, STK11, TP53
Accredited:
ULB-Erasme Pathology solid tumors - gene panels Nicky D’Haene
Sample Type: FFPE Tissue
Frequency: 1x/week
Test Type : Lung Fusion NGS panel (ALK, ROS1, RET and NTRK1 rearrangements) on Ion Torrent PGM (ThermoFisher)
Version date: 11/10/2017
Accredited:
ULB-Erasme Pathology solid tumors - gene panels Nicky D’Haene
Sample Type: FFPE Tissue
Frequency: 1x/week
Test Type : Colon & Lung NGS panel (22 genes : AKT1, ALK, BRAF, CTNNB1, DDR2, EGFR, ERBB2, ERBB4, FBXW7, FGFR1, FGFR2, FGFR3, KRAS, MAP2K1, MET, NOTCH1, NRAS, PIK3CA, PTEN, SMAD4, STK11, TP53) on Ion Torrent PGM (ThermoFisher)
Version date: 11/10/2017
Accredited:
ULB-Erasme Pathology solid tumors - gene panels Nicky D’Haene
Sample Type: FFPE Tissue
Frequency: 1x/week
Test Type : NGS Cancer Panel (50 genes : ABL1, AKT1, ALK, APC, ATM, BRAF, CDH1, CDKN2A, CSF1R, CTNNB1, EGFR, ERBB2, ERBB4, EZH2, FBXW7, FGFR1, FGFR2, FGFR3, FLT3, GNA11, GNAQ, GNAS, HNF1A, HRAS, IDH1, IDH2, JAK2, JAK3, KDR, KIT, KRAS, MET, MLH1, MPL, NOTCH1, NPM1, NRAS, PDGFRA, PIK3CA, PTEN, PTPN11, RB1, RET, SMAD4, SMARCB1, SMO, SRC, STK11, TP53, VHL) on Ion Torrent PGM (ThermFisher)
Version date: 11/10/2017
Accredited:
ULB-Erasme Pathology solid tumors - gene panels Nicky D’Haene
Sample Type: FFPE Tissue
Frequency: 1x/week
Test Type : NGS Gyneco Panel (16 genes : AKT1, BRAF, CDKN2A, CTNNB1, DICER1, ERBB2, FBXW7, FGFR2, FOXL2, KRAS, PIK3CA, PIK3R1, POLE, PTEN, RB1, TP53) on Ion Torrent PGM (ThermoFisher)
Version date: 11/10/2017
Accredited:
Jessa Hospital solid tumors - gene panels Guy Froyen
2x/week
on MiSeq
custom panel
Version date: 13/11/2017
Accredited:
AZ St-Jan Brugge - Pathologie solid tumors - gene panels Dr. Ivo Van Den Berghe - Dr. Jacques Van Huysse - Ing. Stefanie Vermeire
SAMPLE TYPE: FFPE tissues
FREQUENCY: 1X/week
TEST TYPE: NGS
Genes under accreditation: EGFR, KRAS, NRAS, BRAF
Other genes: IDH1, IDH2, KIT, PDGFRA
Version date: 29/11/2017
Accredited: