solid tumors - gene panels

Lab Test name Contact Comments
AZ Delta solid tumors - gene panels Geert Martens/Franceska Dedeurwaerdere/Joke Breyne
In-house NGS panel (SeqCap EZ Choice custom design, KAPA Hyperplus workflow on Miseq) for the detection of mutations, indels, CNV in the following genes:
Lung panel (ALK,BRAF, CDKN2A, DDR2,EGFR,ERBB2,KRAS,MET,PIK3CA,RET,ROS1,TP53)
Colon panel (BRAF,KRAS,NRAS,PIK3CA,TP53)
Melanoma panel (BRAF,KIT,NRAS)
GIST panel (BRAF,KIT,PDGFRA)
BRAIN panel (BRAF,H3F3A,IDH1/2)
BREAST/OVARIAN panel (BRCA1/2,ERBB2,ESR1,PIK3CA,TP53)
Other genes tested but not standard reported: AKT1,APC,ARAF,CTNNB1,ERBB4,GNAS,HRAS,MAP2K1,NF1,NRG1,NTRK1,PIK3R1,PTEN,SMAD4,TERT
Version date: 05/12/2017
Accredited:
UZ Gent solid tumors - gene panels Joni Van der Meulen
Suzanne Vanhauwaert
Kathleen Claes
Nadine Van Roy
Sample Type: FFPE Tissue
Frequency: 1x/week

SOLID MDG DNA NGS panel for detection of SNV and indels (73 genes, KAPA HyperCap Roche): AKT1, ALK, APC, AR, ARID1A, ATM, BAP1, BRAF, BRCA1, BRCA2, CCND1, CDK4, CDK6, CDK12, CDKN2A, CDKN2B, CTNNB1, DICER1, DPYD, EGFR, ERBB2, ERBB3, ESR1, FBXW7, FGFR1, FGFR2, FGFR3, FGFR4, FOXL2, FRK, GATA3, GNA11, GNAQ, GNAS, H3F3A, H3F3B, HIST1B3, HIST1H3C, HNF1A, HRAS, IDH1, IDH2, IL6ST, JAK1, JAK2, KEAP1, KIT, KRAS, MAP2K1, MET, MYOD1, NRAS, NTRK1, NTRK2, NTRK3, PDGFRA, PDGFRB, PIK3CA, PIK3R1, POLE, PTEN, RB1, RET, RNF43, ROS1, SMAD4, SMO, SPOP, STAT3, STK11, TERT, TP53, VHL

BELAC accreditation number (351-MED)
Version date: 16/03/2021
Accredited:
UZ Gent solid tumors - gene panels Joni Van der Meulen
Suzanne Vanhauwaert
Kathleen Claes
Nadine Van Roy
Sample Type: FFPE Tissue
Frequency: 1x/week

SOLID MDG RNA NGS panel for detection of fusions and splice variants (26 genes, Archer FusionPlex): ALK, BRAF, EGFR, ERG, FGFR1, FGFR2, FGFR3, KRAS, MET, MYB, MYBL1, MYC, NRG1, NTRK1, NTRK2, NTRK3, PPARG, PRKCA, RAF1, RELA, RET, ROS1, RSPO2, RSPO3, TMPRSS2, YAP1

BELAC accreditation number (351-MED)
Version date: 16/03/2021
Accredited:
CHU Liège - Hemato solid tumors - gene panels Dr Frédéric LAMBERT, Benjamin Koopmansch, Rafael Carazo
Amplicon sequencing: Tumor Hot Spot Master Plus, Agilent (Multiplicon).
Genes list available at: http://www.chu.ulg.ac.be/jcms/c_9635594/informations-pratiques-relatives-aux-analyses
As suggested by Sciensano / ComPermed: https://www.compermed.be/fr/workflows

BELAC accreditation number: 128-MED
Accredited:
UZ Leuven MolPLUZ solid tumors - gene panels Isabelle Vanden Bempt / Sara Vander Borght
Sample type: FFPE / fresh tissues
Frequency: 2x/ week
Test type: KAPA Hyperplus NGS workflow with custom IDT xGen® Lockdown® Probes on an Illumina sequencing instrument.
Genes: AKT1, ALK, AMER1, APC, ARAF, ARID1A, ATM, ATRX, BAP1, BCOR, BRAF, BRCA1, BRCA2, CCND1, CCNE1, CDH1, CDK4, CDK6, CDKN2A, CIC, CTNNB1, DDR2, DICER1, DPYD, EGFR, ERBB2, ERBB3, ERBB4, ERCC2, ESR1, FBXW7, FGFR1, FGFR2, FGFR3, FGFR4, FOXL2, FUBP1, GATA3, GLI1, GLI2, GNA11, GNAQ, GNAS, H3F3A, H3F3B, HIST1H3B, HIST1H3C, HRAS, IDH1, IDH2, KIT, KRAS, LZTR1, MAP2K1, MDM2, MDM4, MET, mTOR, MYC, MYCN, NF1, NF2, NOTCH1, NRAS, PALB2, PDGFRA, PDGFRB, PIK3CA, PIK3R1, POLE, PRDM6, PTCH1, PTEN, RAC1, RAF1, RB1, RET, RICTOR, RNF43, ROS1, SDHA, SDHB, SDHC, SDHD, SMAD4, SMARCA4, SMARCB1, SMO, SPRED1, STK11, SUFU, TERT, TP53, TSC1, TSC2, VEGFR3 (FLT4), WT1.
Depending on the indication, a subset of the captured regions is examined (see website for more details).
Accredited:
ULB-Erasme Pathology solid tumors - gene panels Nicky D’Haene
Sample Type: FFPE Tissue
Frequency: 1x/week
Test Type : Lung Fusion NGS panel (ALK, ROS1, RET and NTRK1 rearrangements) on Ion Torrent PGM (ThermoFisher)
Version date: 11/10/2017
Accredited:
ULB-Erasme Pathology solid tumors - gene panels Nicky D’Haene
Sample Type: FFPE Tissue
Frequency: 1x/week
Test Type : Colon & Lung NGS panel (22 genes : AKT1, ALK, BRAF, CTNNB1, DDR2, EGFR, ERBB2, ERBB4, FBXW7, FGFR1, FGFR2, FGFR3, KRAS, MAP2K1, MET, NOTCH1, NRAS, PIK3CA, PTEN, SMAD4, STK11, TP53) on Ion Torrent PGM (ThermoFisher)
Version date: 11/10/2017
Accredited:
ULB-Erasme Pathology solid tumors - gene panels Nicky D’Haene
Sample Type: FFPE Tissue
Frequency: 1x/week
Test Type : NGS Cancer Panel (50 genes : ABL1, AKT1, ALK, APC, ATM, BRAF, CDH1, CDKN2A, CSF1R, CTNNB1, EGFR, ERBB2, ERBB4, EZH2, FBXW7, FGFR1, FGFR2, FGFR3, FLT3, GNA11, GNAQ, GNAS, HNF1A, HRAS, IDH1, IDH2, JAK2, JAK3, KDR, KIT, KRAS, MET, MLH1, MPL, NOTCH1, NPM1, NRAS, PDGFRA, PIK3CA, PTEN, PTPN11, RB1, RET, SMAD4, SMARCB1, SMO, SRC, STK11, TP53, VHL) on Ion Torrent PGM (ThermFisher)
Version date: 11/10/2017
Accredited:
ULB-Erasme Pathology solid tumors - gene panels Nicky D’Haene
Sample Type: FFPE Tissue
Frequency: 1x/week
Test Type : NGS Gyneco Panel (16 genes : AKT1, BRAF, CDKN2A, CTNNB1, DICER1, ERBB2, FBXW7, FGFR2, FOXL2, KRAS, PIK3CA, PIK3R1, POLE, PTEN, RB1, TP53) on Ion Torrent PGM (ThermoFisher)
Version date: 11/10/2017
Accredited:
Jessa Hospital solid tumors - gene panels Dr. Brigitte Maes, Guy Froyen
AmpliSeq Focus + custom panel (DNA + RNA)
AmpliSeq BRCA panel
2x/week
on MiSeq
Accredited 275-MED
Version date: 03/08/2021
Accredited:
UZ Brussel - Molecular Pathology solid tumors - gene panels Freya Vaeyens
NGS targeted sequencing after hybridization capture, home brew STHT assay (solid and hematological tumors)
Genes list and indications available at http://www.brightcore.be/stht
Sample Type: FFPE Tissue
Frequency: 1x/week
Accreditation: BELAC 141-MED
Version date: 10/10/2019
Accredited:
AZ St-Jan Brugge - Pathologie solid tumors - gene panels Dr. Ivo Van Den Berghe - Dr. Jacques Van Huysse - Ing. Stefanie Vermeire
SAMPLE TYPE: FFPE tissues
FREQUENCY: 1X/week
TEST TYPE: NGS
Genes under accreditation: EGFR, KRAS, NRAS, BRAF
Other genes: IDH1, IDH2, KIT, PDGFRA
Version date: 29/11/2017
Accredited:
UZ Gent solid tumors - gene panels Joni Van der Meulen
Suzanne Vanhauwaert
Kathleen Claes
Nadine Van Roy
Sample Type: FFPE Tissue
Frequency: 1x/week

SARCOMA MDG RNA NGS panel for detection of fusions and splice variants (40 genes, Archer FusionPlex): ALK, BCOR, BRAF, CAMTA1, CCNB3, CIC, CSF1, EPC1, EWSR1, FGFR1, FGFR2, FGFR3, FOSB, FOXO1, FUS, GLI1, HMGA2, JAZF1, MEAF6, MKL2, NCOA2, NR4A3, NTRK1, NTRK2, NTRK3, NUTM1, PAX3, PDGFB, PHF1, PLAG1, RET, ROS1, SS18, STAT6, TAF15, TCF12, TFE3, TFG, USP6, YWHAE

BELAC accreditation number (351-MED)
Version date: 16/03/2021
Accredited:
UZ Brussel - Molecular Pathology solid tumors - gene panels Freya Vaeyens
Fusion NGS panel - TSO500 Illumina
Sample Type: FFPE tissue
Frequency: 1x/week
Version date: 10/02/2021
Accredited:
Jessa Hospital solid tumors - gene panels Dr. Brigitte Maes, Guy Froyen
Comprehensive genomic tumor profiling (CGP) with TSO500 assay (Illumina)
Detection of SNVs and indels in 523 cancer-related genes, CNVs in 57 genes, gene fusions in 55 driver genes and exon skipping events in 3 relevant genes. Moreover analysis of Tumor Mutation Burden (TMB) and MicroSatellite Instability (MSI) of the tumor specimen is performed.
2x/week
NextSeq500/550; 8 DNA + 8 RNA samples on a High Output flowcell
Accredited 275-MED
Version date: 03/08/2021
Accredited: